Chromatographic separation of brain lipids: cerebroside and sulphatide

Long, Cheryl; Staples, D. A.

doi:10.1042/bj0780179

Cited by 111 publications

(31 citation statements)

References 26 publications

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“…The material was stored at --20 ~ under nitrogen prior to use. Phosphatidylserine (PS) was obtained from Lipid Products and further purified on a silicic acid column according to the method of Long, Shapiro and Staples (1962). 1,2-dimyristoyl-L-phosphatidylcholine was synthesized by Mr. B. Partrick.…”

Section: Methodsmentioning

confidence: 99%

Studies of the conductance changes induced in bimolecular lipid membranes by alamethicin

1972

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The addition of alamethicin to lecithin bilayers results in both voltage-dependent and voltage-independent conductance changes. In the voltage-dependent region, the slope of the conductance-voltage curve varies with the charge of the cation present in the aqueous phase. It may be shown that these effects may be accounted for by a kinetic model which incorporates the following suppositions: (1) alamethicin molecules are adsorbed at the membrane-water interface; (2) the effect of the potential is to redistribute alamethicin-cation complexes between the two surfaces of the bilayer; (3) conduction through the bilayer follows the surface interaction of approximately six alamethicin molecules; and (4) there is an assymetry in the rate constants for corresponding transitions on opposite sides of the bilayer.The effects of alamethicin are found to be approximately the same at neutral and low pH and are unchanged when bilayers are formed from phosphatidyl serine rather than lecithin. These findings are discussed in relation to current hypotheses of the molecular nature of the conduction mechanism.

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Section: Methodsmentioning

confidence: 99%

Studies of the conductance changes induced in bimolecular lipid membranes by alamethicin

1972

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“…Each extracted band of residual lipids was used for analytical methods and 1 H-NMR spectroscopy. Total phosphorus (33), esters (47), nitrogen (20), and glycerol (30) analysis was performed as described previously. Deacylation (27,54) was also performed in 0.1 ml of NaOH (1.2 N) in 50% (vol/vol) methanol at 45°C for 20 min.…”

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confidence: 99%

Coordinated Regulation of Cold-Induced Changes in Fatty Acids with Cardiolipin and Phosphatidylglycerol Composition among Phospholipid Species for the Food Pathogen Listeria monocytogenes

Mastronicolis

Arvanitis

Karaliota

et al. 2008

Appl Environ Microbiol

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We present here the structural identification of four phospholipid (Phl) classes in Listeria monocytogenes, the fatty acid (FA) composition for each individual Phl species, and a description of cold-induced FA changes. Cardiolipin (48.5%) and phosphatidylglycerol (18.1%) are dominated by anteiso-FA, and the previously recognized branched FA chain shortening by cold was observed singularly in these Phls. Phosploaminolipid (19.9%) and phosphatidylinositol, (9.1%) are significantly different, containing significant amounts of straightchain FA. These findings are supported by nuclear magnetic resonance analysis.The bacterium Listeria monocytogenes, one of the leading causes of food-borne illness (15), can adapt to cold and salinity (9,10,31,46,53) and can survive even on dust or flakes of organic material (34). Listeria is characterized by Ͼ85% branched-chain fatty acids (FA), anteiso-15:0 (a-15:0) and anteiso-17:0 (a-17:0). Cells grown in the cold (5°C) contain significantly less a-17:0 than those grown at higher temperatures (4,8,37,43,44,57). A cryotolerance element of Listeria is also its ability to use cryoprotectant osmolytes (31), a property shared by other organisms (2, 3, 12, 21, 23). The critical role of odd numbered anteiso-FA in influencing the lower-temperature growth limits has recently been reported (14,19,25). We have reported previously (39) that Listeria spp. can be cold adapted by an increased content (30%) of neutral lipids (NL) among total lipids (TL) and an increase in the a-15:0/a-17:0 FA ratio (FAr) of TL, polar lipids (PL), and NL (10-, 10-, and 7-fold, respectively), as well as by an increase in FAr (38) for each NL subclasses 1,2-diglyceride, 1,3-diglyceride and free FA (6-, 5-, and 8-fold, respectively).This study is a continuation (35, 36) of an investigation for the structural characterization of the major phospholipid (Phl) classes of L. monocytogenes, in order to (i) elucidate the isolation and quantification of each of class (cells at 30°C), (ii) evaluate the cold temperature (5°C) effect on each individual Phl class FA composition, and (iii) confirm the findings by 1 H-nuclear magnetic resonance (NMR) analysis.

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“…Lipid extracts were prepared for chromatography as described by Long and Staples (1961). Lipid extracts were prepared for chromatography as described by Long and Staples (1961).…”

Section: Chemical Methodsmentioning

confidence: 99%