1973
DOI: 10.1128/jb.113.2.739-753.1973
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Chromatographically Fractionated Complementary Strands of Bacillus subtilis Deoxyribonucleic Acid: Biological Properties

Abstract: Biological, physical, and chromatographic properties of methylated albuminkieselguhr (MAK)-fractionated complementary strands, designated as light (L) and heavy (H), of Bacillus subtilis deoxyribonucleic acid (DNA) are presented. The pattern of transforming activity along the MAK elution profile of alkilidenatured DNA shows that the residually active molecules selectively fractionated ahead of the L strand fraction, whereas the most active self-annealed molecules fractionated preferentially at the trailing end… Show more

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Cited by 9 publications
(14 citation statements)
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“…The procedure for cellular DNA was then followed. The preparation of competent cells and transformations were as described by Rudner and Remeza (15).…”
Section: Methodsmentioning
confidence: 99%
“…The procedure for cellular DNA was then followed. The preparation of competent cells and transformations were as described by Rudner and Remeza (15).…”
Section: Methodsmentioning
confidence: 99%
“…In the preceding paper (24), we characterized the biological, physical, and chromatographic properties of methylated albumin-kieselguhr (MAK)-fractionated complementary strands of Bacillus subtilis deoxyribonucleic acid (DNA). The excellent chromatographic resolution of the fragmented DNA strands accomplished by the technique of intermittent gradient elution (23) has enabled us to study the distribution of marker activities on the MAK elution profile.…”
mentioning
confidence: 99%
“…MATERIALS AND METHODS Bacterial strains, growth of competent cells, and transformation. Strains and transformation procedures were described in the accompanying paper (24).…”
mentioning
confidence: 99%
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