Chromatography, Computer‐Aided Design

Dynamic Capacity Studies of CIM (Convective Interaction Media)® Monolithic Columns

“…The latter is especially important for the purification of molecules on a preparative scale where productivity is essential. Besides the dynamic binding capacity, which determines the cycle time, also the shape of the breakthrough curve and its dependence on the ionic strength of the solution has a major influence on productivity [24]. At high ionic strength, there is a competitive adsorption between macromolecules and ions at active sites, which decreases the binding capacity of a target molecule [25].…”

Bioseparations

“…A major problem is the similarity between the doublestranded DNA plasmids, genetic DNA, and RNA, but the large size of plasmids, with typical sizes of 3,000 to 20,000 base pairs, presents major difficulties as well. Capacity of conventional adsorbents designed for protein separations is typically very small, and only a very few of available adsorbents appears suitable (Deshmukh and Warner, 2000;Jungbauer and Kaltenbrunner, 1999;Teeters et al, 2002).…”

“…Statistical techniques for evaluating process performance are being refined (Blackmore and Ryland, 2003;Larson et al, 2002Larson et al, , 2003Prior et al, 2003;Sonnenfeld et al, 2003;Taggart et al, 2003). Computerized design techniques (Jungbauer and Kaltenbrunner, 1999) continue to be developed by a number of research groups (Harrison et al, 2003, Ch. 11;Mustafa et al, 2003;Nagrath et al, 2003;Pieracci et al, 2003).…”