Chromatography of 3-hydroxypyridin-4-ones: novel orally active iron chelators

Epemolu, R.O.; Singh, Surinder; Hider, Robert C.; Damani, L. A.

doi:10.1016/0021-9673(90)85145-l

Cited by 28 publications

(9 citation statements)

References 12 publications

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“…It was previously reported that chromatography of HPOs on a C18 column resulted in broad, asymmetrical and tailing peaks [3,4]. Polymeric columns, offer a number of advantages, including pH stability from 1 to 13, excellent sample recoveries due to lack of acidic silanol groups, compatibility with the mobile phases containing 0-100% organic solvent, and greater surface area than alkyl bonded silicas, were found to be more suitable for HPOs chromatography [6].…”

Section: Methods Selectivity and Robustnessmentioning

confidence: 98%

“…Previously reported reverse-phase high-performance liquid chromatographic method (RP-HPLC) methods for analysis of highly hydrophilic HPOs in biological materials were unsatisfactory due to their poor resolution from interfering endogenous substances [3]. Therefore, Skinner et al suggested RP-HPLC combined with a baseline subtraction method [4].…”

Section: Introductionmentioning

confidence: 99%

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Improved high-performance liquid chromatographic method for the pharmacokinetic studies of a novel iron chelator, CP502, in rats

Novaković

Tesoro

Spino

et al. 2003

Journal of Chromatography B

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Section: Methods Selectivity and Robustnessmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Improved high-performance liquid chromatographic method for the pharmacokinetic studies of a novel iron chelator, CP502, in rats

Novaković

Tesoro

Spino

et al. 2003

Journal of Chromatography B

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“…So monitoring of drug plays pivotal role in clinical applications. Various methods are available for analysis of drug like chromatography (Epemolu et al, 1990), HPLC (Goddard and Kontoghiorghes, 1990) and liquid chromatography coupled with mass spectrometry (LC/ MS) (Biancamano et al, 2009). But these methods have many setbacks like time consuming preparation, skilled person to operate, bulky instruments and high cost.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical impediometric detection of anti-HIV drug taking gold nanorods as a sensing interface

Narang

Malhotra

Singh

et al. 2015

Biosensors and Bioelectronics

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“…It was previously found that the chromatography of the 3-hydroxypyridin-4-ones is difficult on normal octadecylsilica (ODS) columns as the analytes are often characterized by broad asymmetrical and sometimes multiple peaks. 13 Goddard and Kontoghiorghes 14 described a HPLC based method to assay deferiprone in serum and urine. Dresow et al 15 also describe a HPLC method for the determination of deferiprone in serum and urine samples and a HPLC based assay to determine the iron excretion (due to deferiprone therapy) by measuring urinary Fe-(deferiprone) 3 complex concentration.…”

Section: Introductionmentioning

confidence: 99%

Electrochemistry of deferiprone as an orally active iron chelator and HIV-1 replication inhibitor and its determination

Yadegari¹,

Jabbari²,

Heli³

et al. 2008

J. Braz. Chem. Soc.

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O comportamento eletroquímico da deferiprona, droga anti-talassêmica e anti-replicação do HIV foi investigado, por voltametria cíclica (VC), utilizando um eletrodo de platina. Observaram-se dois picos anódicos irreversíveis para a deferiprona, em tampão acetato (pH 4,0) com E 0 1 = 875 mV e E 0 2 = 1235 mV (vs. Ag/AgCl) em velocidade de varredura de 50 mV s -1 . Os estudos de voltametria cíclica indicaram que o processo de oxidação é irreversível e controlado por difusão. Foram encontrados, para a deferiprona, coeficientes de difusão e de transferência de elétrons de 3,50 × 10 -6 cm 2 s -1 e 0,57, respectivamente. Foi desenvolvido um procedimento sensível, simples e rápido, utilizando voltametria de pulso diferencial. Utilizando este método, a deferiprona pode ser determinada com um limite de detecção de 1,43 × 10 -5 mol L -1 . É descrita a aplicabilidade do método em ensaios diretos em soro humano enriquecido, fluidos urinários e em comprimidos comerciais.The electrochemical behavior of the anti-thalassemia and anti-HIV replication drug, deferiprone, was investigated by cyclic voltammetry (CV) at a platinum electrode. In an acetate buffer solution, pH = 4.0, two irreversible anodic peaks for deferiprone, with E 0 1 = 875 mV and E 0 2 = 1235 mV (vs. Ag/AgCl) appeared at a potential sweep rate of 50 mV s -1 . Cyclic voltammetric study indicated that the oxidation process is irreversible and diffusion-controlled. The diffusion and the electron transfer coefficients of deferiprone were found to be 3.50 × 10 -6 cm 2 s -1 and 0.57, respectively. A sensitive, simple and time-saving differential pulse voltammetric procedure was developed. Using our proposed method, deferiprone can be determined with a detection limit of 1.43 × 10 -5 mol L -1 . The applicability of the method to direct assays of spiked human serum and urine fluids, and to commercial tablets, is described.Keywords: deferiprone, anti-thalassemia drug, platinum electrode, iron chelator, HIV-1 replication inhibitor IntroductionDeferiprone (1,2-dimethyl-3-hydroxypyridin-4-one, Scheme 1) is the first oral iron chelator to be used clinically, mainly in thalassemia patients. 1,2 Deferiprone belongs to the family of the alpha-ketohydroxypyridines, a relatively new class of chelating agents, some of which are naturally occurring. These chelators have a high affinity for binding iron, and are able to remove it from proteins that are transporting and storing it in the body, largely sparing other biologically important metals. They are stable in conditions that exist in the human digestive system and are readily absorbed. Deferiprone can remove excess iron from various parts of the body of iron-loaded patients, including liver and, particularly, heart. 1 This drug is also used worldwide to treat cancer, leukemia, in hemodialysis and other diseases. It is worthy noting that the drug deferiprone may be used in the detoxification of other metals, such as aluminum in hemodialysis patients, plutonium used in the nuclear industry and uranium used by the military. [3][4][5...

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Chromatography of 3-hydroxypyridin-4-ones: novel orally active iron chelators

Cited by 28 publications

References 12 publications

Improved high-performance liquid chromatographic method for the pharmacokinetic studies of a novel iron chelator, CP502, in rats

Improved high-performance liquid chromatographic method for the pharmacokinetic studies of a novel iron chelator, CP502, in rats

Electrochemical impediometric detection of anti-HIV drug taking gold nanorods as a sensing interface

Electrochemistry of deferiprone as an orally active iron chelator and HIV-1 replication inhibitor and its determination

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