Chromatography of Anthocyanins on Columns of Insoluble Polyvinylpyrrolidone

Teeling, Cornelis G. Van; Cansfield, P. E.; Gallop, R.A.

doi:10.1093/chromsci/9.8.505

Cited by 26 publications

(6 citation statements)

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“…Mobile phase, 60/40 (v/v) MeOH/water; flow rate, 1.00 mL/min; UV detection at 254 nm; 0.32 AUFS. Peak identities: (1) benzene, (2) biphenyl, (3) naphthalene, (4) fluorene, (5) phenanthrene, (6) anthracene, (7) benz[a]anthracene, (8) benzo[a]pyrene carbon atoms (noncondensed), such as p-terphenyl, are not retained as strongly as ring systems which share two carbon atoms (catacondensed) such as anthracene. PAHs with three carbon atoms shared (pericondensed), such as pyrene, are not retained as strongly as catacondensed systems (typically, chrysene).…”

Section: Resultsmentioning

confidence: 99%

High performance liquid chromatographic separation of polycyclic aromatic hydrocarbons on microparticulate pyrrolidone and application to the analysis of shale oil

Mourey¹,

Siggia²,

Uden³

et al. 1980

Anal. Chem.

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Section: Resultsmentioning

confidence: 99%

High performance liquid chromatographic separation of polycyclic aromatic hydrocarbons on microparticulate pyrrolidone and application to the analysis of shale oil

Mourey¹,

Siggia²,

Uden³

et al. 1980

Anal. Chem.

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“…other polyphenolic substances, 272 NATURAL FOOD COLORANTS pectin) that could influence the stability and/or analysis of these pigments. Co-extracted flavonoids, which react similarly with the common reagents used for phenolic analysis, are commonly removed via chromatographic techniques employing insoluble polyvinylpyrrolidone (PVP) [324][325][326], polyamide [327], combination polyamide-PVP [59,328,329]. Sephadex G-25 [101] or LH-20 [101,330,331], octadecylsilane [101,331,332], weak anion exchange (e.g.…”

Section: Purificationmentioning

confidence: 99%

Natural Food Colorants

1996

111

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Preface to the second editionIn this second edition of Natural Food Colorants two new chapters have been added and we have taken the opportunity to revise all the other chapters. Each of the original authors have brought up to date their individual contributions, involving in several cases an expansion to the text by the addition of new material. The new chapters are on the role of biotechnology in food colorant production and on safety in natural colorants, two areas which have undergone considerable change and development in the past five years. We have also persuaded the publishers to indulge in a display of colours by including illustrations of the majority of pigments of importance to the food industry. Finally we have rearranged the order of the chapters to reflect a more logical sequence. We hope this new edition will be greeted as enthusiastically as the first.It remains for us, as editors, to thank our contributors for undertaking the revisions with such thoroughness and to thank Blackie A&P for their support and considerable patience.

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“…Perhaps an explanation for the different affinities is found in the higher tyrosine content of PRPl (1 6%) versus PRP2 (1 1 O/.). PVPP is commonly used to remove pheriolics from plant tissues because it binds to the phenolic hydroxyl residues (Loomis, 1974), and it also has been used to purify flavonoids and anthocyanins (Van Teeling et al, 1971). If the very high tyrosine content enables PRPI to bind PVPP through the tyrosyl hydroxyl groups, then PRP2 might not bind because its tyrosine content is lower.…”

Section: Prp1 But Not Prps Binds To Lnsoluble Pvppmentioning

confidence: 99%

A soybean cell wall protein is affected by seed color genotype.

Lindstrom

Vodkin

1991

Plant Cell

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, lllinois 61 801The dominant I gene inhibits accumulation of anthocyanin pigments in epidermal cells of the soybean seed coat. We compared saline-soluble proteins extracted from developing seed coats and identified a 35-kilodalton protein that was abundant in Richland (genotype I / / , yellow) and much reduced in an isogenic mutant line T157 (genotype ili, imperfect black seed coats). We purified the 35-kilodalton protein by a nove1 procedure using chromatography on insoluble polyvinylpolypyrrolidone. The 35-kilodalton protein was composed primarily of proline, hydroxyproline, valine, tyrosine, and lysine. Three criteria (N-terminal amino acid sequence, amino acid composition, and sequence of a cDNA) proved that the seed coat 35-kilodalton protein was PRPI, a member of a proline-rich gene family expressed in hypocotyls and other soybean tissues. The levels of soluble PRPI polypeptides and PRPI mRNA were reduced in young seed coats with the recessive ili genotype. These data demonstrated an unexpected and nove1 correlation between an anthocyanin gene and the quantitative levels of a specific, developmentally regulated cell wall protein. In contrast, PRPS, a closely related cell wall protein, was synthesized later in seed coat development and was not affected by the genotype of the I locus.

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Chromatography of Anthocyanins on Columns of Insoluble Polyvinylpyrrolidone

Cited by 26 publications

References 0 publications

High performance liquid chromatographic separation of polycyclic aromatic hydrocarbons on microparticulate pyrrolidone and application to the analysis of shale oil

High performance liquid chromatographic separation of polycyclic aromatic hydrocarbons on microparticulate pyrrolidone and application to the analysis of shale oil

Natural Food Colorants

A soybean cell wall protein is affected by seed color genotype.

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