Chromatography of deoxyribonucleic acids on calcium phosphate columns

Main, Raymond K.; Cole, Leonard J.

doi:10.1016/0003-9861(57)90339-9

Cited by 29 publications

(2 citation statements)

References 6 publications

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“…The first chromatographic separations of DNA were performed on calcium phosphate columns by phosphate buffer elution in 1957 by Main and Cole who successfully separated photodegraded nucleotide fragments from untreated DNA (Main and Cole, 1957). For this method, cellular DNA is extracted in a series of steps beginning with cell lysis of a centrifuged cell pellet on ice with a protease, for example protease K, addition of sodium acetate to stabilize the helix, and precipitation of DNA by addition of cold isopropanol before lyophilization and resuspension in a buffer (Pfeifer, 1996).…”

Section: Methods For Sequencing Dna and Identification Of Dna Admentioning

confidence: 99%

Tandem mass spectrometry for characterization of covalent adducts of DNA with anticancer therapeutics

Silvestri

Brodbelt

2012

Mass Spectrometry Reviews

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The chemotherapeutic activities of many anticancer and antibacterial drugs arise from their interactions with nucleic acid substrates. Some of these ligands interact with DNA in a way that causes conformational changes or damage to the nucleic acid targets, ultimately altering recognition by key DNA-specific enzymes, interfering with DNA transcription or prohibiting replication, and terminating cell growth and proliferation. The design and synthesis of ligands that bind to nucleic acids remains a dynamic field in medicinal chemistry and pharmaceutical research. The quest for more selective and efficacious DNA-interactive anti-cancer chemotherapeutics has likewise catalyzed the need for sensitive analytical methods that can provide structural information about the nature of the resulting DNA adducts and provide insight into the mechanistic pathways of the DNA/drug interactions and the impact on the cellular processes in biological systems. This review focuses on the array of tandem mass spectrometric strategies developed and applied for characterization of covalent adducts formed between DNA and anti-cancer ligands.

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Section: Methods For Sequencing Dna and Identification Of Dna Admentioning

confidence: 99%

Tandem mass spectrometry for characterization of covalent adducts of DNA with anticancer therapeutics

Silvestri

Brodbelt

2012

Mass Spectrometry Reviews

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“…τήξης: Ή μελέτη της συμπεριφοράς των προϊόντων της υβριδοποίησης ώς προς τήν θερμική τους σταθερότητα πραγματοποιήθηκε σέ στήλη ύδροξυαπατίτη (ΗΑΡ). Ή μέθοδος τού ύδροξυαπατίτη (μορφή φωσφορικού ασβεστίου) αρχικά άναπτύχτηκε γιά τή χρωματογραφία πρωτεϊνών (Tiselius et al, 1956) άλλα στη συνέχεια χρησιμοποιήθηκε στά νουκλεϊκά οξέα άπό τόν Semenza, 1957 και άπό άλλους ερευνητές (Main et al., 1957(Main et al., , 1959. Γενικά έχει αποδειχτεί σάν μιά αξιόλογη μέθοδος γιά καθαρισμό και κλασμάτωση πρωτεϊνών, ένζυμων, νουκλεϊκών οξέων, ιών και άλλων μακρομορίων.…”

Section: καμπύλεςunclassified

Εξελικτικη Συγκριση Των Δομικων Και Ρυθμιστικων Γονιδιων Του Χοριου Σε Δυο Ειδη Αγριου Μεταξοσκωληκα

Μοσχονασ¹

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The large scale preparation of chromatographic grade hydroxylapatite and its application in protein separation procedures

Atkinson¹,

Bradford²,

Selmes³

1973

J. Appl. Chem.

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Methods are described for both the laboratory and plant scale preparations of hydroxylapatite to give 200 ml and 22 litre settled bed volumes respectively. These materials have an average particle size of 75 pm, give flow rates of up to 45 ml cm-2h-1 and bind up to 50 mg protein/ml hydroxylapatite at pH 6.8. Examples of protein separations and purifications on hydroxylapatite are given and the nature and basis of hydroxylapatite chromatography is discussed.

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Chromatography of deoxyribonucleic acids on calcium phosphate columns

Cited by 29 publications

References 6 publications

Tandem mass spectrometry for characterization of covalent adducts of DNA with anticancer therapeutics

Tandem mass spectrometry for characterization of covalent adducts of DNA with anticancer therapeutics

Εξελικτικη Συγκριση Των Δομικων Και Ρυθμιστικων Γονιδιων Του Χοριου Σε Δυο Ειδη Αγριου Μεταξοσκωληκα

The large scale preparation of chromatographic grade hydroxylapatite and its application in protein separation procedures

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