Chromatography of nitrofluoresceins, aminofluoresceins, and fluorescein isothiocyanates

Fluorometric Analysis

“…The specific identification of proteins or microorganisms by their immunochemical reaction with fluorescence-labeled antibodies has become so valuable that a book has been published on this and other aspects of fluorescent protein tracing (342). Efforts continue to be expended on such improvements as selecting the brightest fluorochromes for the labeling (103,171,172,388), and removal of nonspecific staining material by column chromatography (92,298,400). Fluorescein isocyanate was found to give twice the emission intensity of the isothiocyanate, in the case of the rabbit serum antibody against bovine serum albumin (298).…”