Chromatography of Organic Acids in Cured Tobacco

The Chemical Constituents Of Tobacco And Tobacco Smoke

“…Citric, malic, and oxalic acids were early isolated and identified in the fresh leaf of N. glauca (305) and N. tabacum (362) and in processed tobacco (296, 302), although the absence of oxalic acid has been reported in fresh leaf (413). Chromatography on paper and silicic acid has proved invaluable for the rapid determination of these acids, and the use of such techniques has enabled their presence to be demonstrated in Bulgarian leaf (176), flue-cured Canadian tobacco (125), green leaf (90), green and cured Japanese tobaccos (371), and a cured tobacco (264). It has been shown that malic acid occurs exclusively in N. tabacum leaf as its levorotatory form (363), whilst a similar form was isolated from N. glauca (305).…”

Determination of volatile organic acids in tobacco by single‐drop microextraction with in‐syringe derivatization followed by GC‐MS

“…Organic acids are present in both green tobacco plants and cured leaves, and the major organic acids in tobacco are citric, malic, oxalic and acetic 1, 2. These organic acids can be divided as volatile acids, non‐volatile acids and semi‐volatile acids, which play an important role in both growth and curing procedure of tobacco, especially volatile organic acids.…”

Influence of Environment on Metabolism of Organic Acids and Carbohydrates in Vitis Vinifera. I. Temperature

“…The resin was previously converted to the CO3-form to avoid decomposition of the sugars (22). The column was washed with 3 bed-volumes of distilled water and the washings were combined with the effluent.…”