Chromatography of twentysix sesquiterpene lactones fromCentaurea bella

Nowak, Gerard

doi:10.1007/bf02277519

Cited by 13 publications

(9 citation statements)

References 7 publications

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“…In our previous papers (Cis et al 2000;Daniewski et al 1982;Horoszkiewicz-Hassan and Nowak 2001;Nowak et al 1986;Nowak 1993), it could be demonstrated that the above-mentioned plant species, except for Centaurea cyanus and Serratula wolffii, accumulate guaiane-and/or germacrane-type sesquiterpene lactones. Furthermore, it could be shown that Centaurea bella possesses the highest capacity of producing not only syringin, but also sesquiterpene et al 1979et al Nowak G. 1993 Horoszkiewicz-Hassan M., Nowak G. 2001Nowak G. et al 1986Daniewski W. et al 1982Cis J. et al 2000Nowak G. et al 1986Nowak G. et al 1986 *Estimated on the basis of isolated amounts of syringin from dried plant materials. lactones.…”

Section: Resultsmentioning

confidence: 90%

Syringin in some species of the subtribe Centaureinae of the Asteraceae

et al. 2011

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The occurrence of syringin in eight out of ten investigated plant species of the subtribe Centaureinae (Asteraceae) was reported. The compound was isolated from aerial parts of the plants by silica gel column chromatography of methanol extracts. Contents of syringin varied from 0.001 to 0.1% of the dried plant material. Centaurea bella Trautv. appeared to be the best source of this compound (0.1% dry wt.). Syringin has been reported for the first time from these plant species

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Section: Resultsmentioning

confidence: 90%

Syringin in some species of the subtribe Centaureinae of the Asteraceae

et al. 2011

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“…The characteristic colour of the spots after spraying with the anisaldehyde reagent of the separated compounds from the aerial parts of Stizolophus balsamita [5], Centaurea bella [3,6], Zoegea baldschuanica [4], Centaurea crocodylium and Centaurea lusitanica [6] can be ascribed to the presence or lack of several substituents at C8 (Fig. 2).…”

Section: Germacranolidesmentioning

confidence: 99%

“…The possibility of identification of several substituents at guaianolides by TLC with concentrated sulphuric acid as the developer had been mentioned earlier [2,3]. Now, visual chromatography of compounds of four other types of sesquiterpenoids has been presented: germacranolides, germacranes, seco-pseudoguaianolides and pseudoguaianolides.…”

Section: Introductionmentioning

confidence: 99%

“…A recent study has revealed antibacterial and cytotoxic activities of sesquiterpene lactones [1]. These compounds can be detected through visual chromatography and isolated by simple column chromatography, with silica gel as the adsorbent.The possibility of identification of several substituents at guaianolides by TLC with concentrated sulphuric acid as the developer had been mentioned earlier [2,3]. Now, visual chromatography of compounds of four other types of sesquiterpenoids has been presented: germacranolides, germacranes, seco-pseudoguaianolides and pseudoguaianolides.…”

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confidence: 99%

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TLC of selected sesquiterpenoids of the Asteraceae family

et al. 2011

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IntroductionAmong natural compounds in the species of family Asteraceae, sesquiterpenoids play an important role. They constitute a valuable chemotaxonomic material and might be also decisive for the medicinal value of the raw material, as in Chamomillae anthodium, Cnici benedicti herba, Arnicae anthodium, Millefolii herba, Chrysanthemi partheni herba, Cichorii radix et herba. A recent study has revealed antibacterial and cytotoxic activities of sesquiterpene lactones [1]. These compounds can be detected through visual chromatography and isolated by simple column chromatography, with silica gel as the adsorbent.The possibility of identification of several substituents at guaianolides by TLC with concentrated sulphuric acid as the developer had been mentioned earlier [2,3]. Now, visual chromatography of compounds of four other types of sesquiterpenoids has been presented: germacranolides, germacranes, seco-pseudoguaianolides and pseudoguaianolides.To develop the spots, the sole anisaldehyde reagent, which proved selective enough to rate the colours of the spots of the compounds in question, was used. Material and methods Plant materialThe plant materials used for the studies employed aerial parts of Centaurea bella, C. crocodylium, C. lusitanica, Helenium hoopesii, Santolina pinnata subsp. neapolitana, Stizolophus balsamita, Zoegea baldschuanica, plants cultivated and identified in the garden of Department of Medicinal and Cosmetic Natural Products, University of Medical Sciences in Poznań (Poland), where their voucher specimens are deposited. Isolation and identification of compoundsThe herbs, collected right before blooming (about 450 g each) were extracted with methanol three times. The methanol extracts were inundated with distilled water (ca. 600 cm 3 ), after the evaporation of the solvent. The water phase was extracted with chloroform. The chloroform extracts, in turn, having been dried with anhydrous sodium sulphate, were used for thin layer chromatography and isolation of compounds. Furthermore, some methanol/water/chloroform extracts were prepared from Centaurea crocodylium herb, which had been collected at the same time and from the same plot as the herbs designed to be dried.The extracts were separated by column chromatography on silica gel (Merck art. 7733). The fractions were subjected to repeated column chromatography on silica gel (Merck art. 7729) and eluted by right mobile phases. The structures of the isolated compounds (Fig. 1) were identified on the basis of 1H NMR, IR and EI mass spectroscopy and by comparing the obtained data with those of the reference compounds or reported data [4-8]. AbstractVisual chromatography has been employed for a preliminary identification of natural compound of a group of sesquiterpene lactones -Centaurea bella Trautv., C. crocodylium L., C. lusitanica Boiss. et Reuter, Helenium hoopesii A. Gray = Dugaldia hoopesii (A. Gray) Rydb., Stizolophus balsamita (Lam.) Cass., Zoegea baldschuanica C. Winkl., germacranes -Santolina pinnata Viv. subsp. neapolitana (Jord. et ...

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“…We investigated the number, nature and biological activity of SLs in the different species and summarized some of them in Table 1 (Bruno et al, 2005;Chicca et al, 2011;Csupor-Löffler et al, 2009;El-Najjar et al, 2007;Ghantous et al, 2007;González et al, 1980;Koukoulista et al, 2002;Saroglou et al, 2005). Nowak et al, 1993-3: Cho et al, 2004-4: Medjroubi et al, 2005-5: Bach et al, 2011-6: Vajs et al, 1999-7: Djeddi et al, 2007, 2008-8: González et al, 1980-9: Ozçelik et al, 2009-10: Youssef et al, 1994, 1998-11: Akkol et al, 2011-12: Cheng et al, 1992-13: Gürbüz et al, 2007-14: Ozçelik et al, 2009-15: Saroglou et al, 2005-16: Lakhal et al, 2010-17: Fortuna et al, 2001-18: Lonergan et al, 1992 (Fig. 2a).…”

Section: Sesquiterpene Lactones In Traditional and Conventional Medicinementioning

confidence: 99%