Chromene suppresses the activation of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 cells

Heo, Soo‐Jin; Jang, Jae Yool; Ye, Bo-Ram; Kim, Min-Sun; Yoon, Weon‐Jong; Oh, Chulhong; Kang, Do‐Hyung; Lee, Ji-Hyeok; Kang, Min‐Cheol; Jeon, You‐Jin; Kang, Sung-Myung; Kim, Daekyung; Kim, Kil-Nam

doi:10.1016/j.fct.2014.02.023

Cited by 30 publications

(28 citation statements)

References 32 publications

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“…On the other hand, LPS also acts as an endotoxin and trigger a variety of the immune response in mammalian cells. The inflammatory reaction is stimulated through release of inflammatory mediators by RAW 264.7 macrophages cells when it is exposed to LPS [24]. Upon LPS recognition, LPS is transferred to CD14 by LPS binding protein and recognized by TLR4-MD-2 complex on the cellular surface leading to the rapid and coordinated activation of various intracellular signaling pathways including translocation of NF-κB [25].…”

Section: Resultsmentioning

confidence: 99%

Therapeutic effects of total steroid saponin extracts from the rhizome of Dioscorea zingiberensis C.H.Wright in Freund's complete adjuvant induced arthritis in rats

Zhang¹,

Ito²,

Liang³

et al. 2014

International Immunopharmacology

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The aim of our present study is to explore the anti-arthritic potential effect of total steroid saponins (TSSN) extracted from the rhizome of Dioscorea zingiberensis C.H.Wright (DZW) and to investigate the underlying mechanisms. This work was performed using adjuvant-induced arthritis (AIA) rats in vivo and lipopolysaccharide (LPS) simulated 264.7 macrophage cells in vitro. In AIA-induced arthritic rats, TSSN significantly alleviated the arthritic progression through evaluating arthritic score, immune organ indexes, paw swelling, and body weight. This phenomenon was well correlated with significant suppression of the overproduction of inflammation cytokines (IL-1, IL-1β, IL-6, and TNF-α), oxidant stress makers (MDA and NO), eicosanoids (LTB4 and PGE2), and inflammatory enzymes (5-LOX and COX-2) versus the AIA rats without treatment. On the contrary, the release of SOD and IL-10 was profoundly increased. What’s more, TSSN could obviously ameliorate the translocation of NF-κB to the nucleus through phosphorylation of the p65 and IκBα in vivo and vitro. The current findings demonstrated that TSSN could protect the injured ankle joint from further deterioration and exert its satisfactory anti-arthritis properties through anti-inflammatory and anti-oxidant effects via inactivating NF-κB signal pathway. This research implies that DZW may be a useful therapeutic agent for the treatment of human arthritis.

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Section: Resultsmentioning

confidence: 99%

Therapeutic effects of total steroid saponin extracts from the rhizome of Dioscorea zingiberensis C.H.Wright in Freund's complete adjuvant induced arthritis in rats

Zhang¹,

Ito²,

Liang³

et al. 2014

International Immunopharmacology

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“…We can also find reports on the isolation of chromenes [58,60,62,64,65,70,71,72,73,74,75,76,77]. Their structures are in Figure 11 and occurrences are in Table 3.…”

Section: Structural Characterization and Biological Activitymentioning

confidence: 87%

“…Compounds 101 – 106 significantly decreased generation of intracellular ROS and inhibited lipid peroxidation while they increased levels of intracellular GSH at a concentration of 5 μg/mL. Compound 101 was also isolated by Heo [72] and its anti-inflammatory activity against lipopolysaccharide-exposed RAW 264.7 cells was evaluated. Jang [73] reported the isolation of 101 and 102 , together with 107 – 120 .…”

Section: Structural Characterization and Biological Activitymentioning

confidence: 99%

Secondary Metabolites and Biological Activity of Invasive Macroalgae of Southern Europe

et al. 2018

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In this review a brief description of the invasive phenomena associated with algae and its consequences on the ecosystem are presented. Three examples of invasive algae of Southern Europe, belonging to Rodophyta, Chlorophyta, and Phaeophyta, were selected, and a brief description of each genus is presented. A full description of their secondary metabolites and biological activity is given and a summary of the biological activity of extracts is also included. In Asparagopsis we encounter mainly halogenated compounds. From Caulerpa, several terpenoids and alkaloids were isolated, while in Sargassum, meroterpenoids prevail.

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“…This response is initiated when body tissues are damaged by bacteria, trauma, toxins, heat, or any other agent [1]. Inflammatory mediators, which include prostaglandins, leukotrienes, histamines and bradykinins, secreted by the inflamed organs of the body propagate the inflammation [2]. In response to the inflammatory reactions, tissue manifests swelling pain, oedema and hyperthermia.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of immunomodulatory activity of tenoxicam in mice

Nasim¹,

Javeed²,

Ashraf³

et al. 2018

Trop. J. Pharm Res

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Purpose: The present study was conducted to evaluate the effect of tenoxicam on cellular and humoral immunity. Methods: Tenoxicam (2.5-10mg/kg) was administered at three different doses to three groups of mice and the cellular immune responses were studied using delayed hypersensitivity response (DTH) and cyclophosphamide-induced neutropenia while the humoral immune response was evaluated using hemagglutination test and mice mortality ratio. Normal saline and cyclophosphamide were used as negative and positive controls, respectively. Results: DTH assay resulted in a significant reduction in skin thickness (p < 0.05) for tenoxicam treated groups when compared to the negative control group at 24 h, 48 h and 72 h after administration of challenging dose of dinitrochlorobenzene (DNCB). Cyclophoshamide induced neutropenia showed a significant percentage reduction in total leukocyte count (TLC) and differential leukocyte count (DLC) i.e. lymphocytes and neutrophils (p< 0.05), but an increase in monocytes in all the treatment groups in the following order: 10 mg>5 mg >2.5 mg> negative control group. A dose dependent reduction response was observed (p<0.05) in haemagglutination assay (HA). In mice lethality test mortality ratios of 2.5 mg, 5 mg, 10 mg tenoxicam were 60 %, 80% and 100 %, respectively, compared to 20 % and 100 % for normal saline group and cyclophosphamide, respectively Conclusion: The results suggest that tenoxicam suppresses both cellular and humoral immunity in mice.

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Chromene suppresses the activation of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 cells

Cited by 30 publications

References 32 publications

Therapeutic effects of total steroid saponin extracts from the rhizome of Dioscorea zingiberensis C.H.Wright in Freund's complete adjuvant induced arthritis in rats

Therapeutic effects of total steroid saponin extracts from the rhizome of Dioscorea zingiberensis C.H.Wright in Freund's complete adjuvant induced arthritis in rats

Secondary Metabolites and Biological Activity of Invasive Macroalgae of Southern Europe

Evaluation of immunomodulatory activity of tenoxicam in mice

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