Chromium effects on photosynthetic electron transport in pea (Pisum sativum L.)

Todorenko, D. A.; Тимофеев, Н. П.; Kovalenko, I. B.; Kukarskikh, G. P.; Маторин, Д. Н.; Antal, Taras K.

doi:10.1007/s00425-019-03304-1

Cited by 29 publications

(16 citation statements)

References 60 publications

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“…In good agreement with the conclusion that upon Cr treatment of the cells, the strongest effects on the photochemical apparatus are found around the cytochrome b/f complex and PSI ( Todorenko et al., 2020 ), we also found that the linear electron transport rate (ETR) and the photooxidizable concentration of P700 are severely inhibited ( Figures 4A, B , respectively). These inhibitory effects on ETR and on the activity of PSI were less pronounced with Cd.…”

Section: Resultssupporting

confidence: 90%

“…In the presence of Cr, the OJIP curves exhibited different features compared to the control and the Cd-treated samples ( Figure 2B ): (i) after 12 h of the treatment, the fluorescence rise was dominated by the OJ phase and the contribution of the JI and IP phases were unusually small—a rise kinetics similar to that obtained on 50 μM Cr-treated pea after 96 h ( Todorenko et al., 2020 ), which indicated that the earliest effect on the photosynthetic electron transport chain was in the intersystem electron transport; (ii) similar to the OJIP transients on Cr-treated pea, we also observed a pronounced rise of the F o level to 44% at 12 h (data not shown), which was attributed to the partial reduction of Q A ( Todorenko et al., 2020 ); (iii) in later phases of the toxic effects, the JI and IP phase were absent and the F o level, which probably also contained contributions from inactive PSII complexes, rose further, to 76% (t=24 h) and 93% (t=48 h), virtually eliminating the F v . It is interesting to note that the fluorescence rise observed in the sample treated with high concentration (50 mg L −1 , corresponding to 962 μM) Cr also closely resembles the transient of M. denticulata treated with 10 μM Cr(VI) for 3 weeks ( Volland et al., 2012 ).…”

Section: Resultsmentioning

confidence: 73%

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Similarities and Differences in the Effects of Toxic Concentrations of Cadmium and Chromium on the Structure and Functions of Thylakoid Membranes in Chlorella variabilis

et al. 2020

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Trace metal contaminations in natural waters, wetlands, and wastewaters pose serious threats to aquatic ecosystems—mainly via targeting microalgae. In this work, we investigated the effects of toxic amounts of chromium and cadmium ions on the structure and function of the photosynthetic machinery of Chlorella variabilis cells. To halt the propagation of cells, we used high concentrations of Cd and Cr, 50–50 mg L −1 , in the forms of CdCl 2 x 2.5 H 2 O and K 2 Cr 2 O 7 , respectively. Both treatments led to similar, about 50% gradual diminishment of the chlorophyll contents of the cells in 48 h, which was, however, accompanied by a small (~10%) but statistically significant enrichment (Cd) and loss (Cr) of ß-carotene. Both Cd and Cr inhibited the activity of photosystem II (PSII)—but with more severe inhibitions with Cr. On the contrary, the PsbA (D1) protein of PSII and the PsbO protein of the oxygen-evolving complex were retained more in Cr-treated cells than in the presence of Cd. These data and the higher susceptibility of P700 redox transients in Cr-treated cells suggest that, unlike with Cd, PSII is not the main target in the photochemical apparatus. These differences at the level of photochemistry also brought about dissimilarities at higher levels of the structural complexity of the photosynthetic apparatus. Circular dichroism (CD) spectroscopy measurements revealed moderate perturbations in the macro-organization of the protein complexes—with more pronounced decline in Cd-treated cells than in the cells with Cr. Also, as reflected by transmission electron microscopy and small-angle neutron scattering, the thylakoid membranes suffered shrinking and were largely fragmented in Cd-treated cells, whereas no changes could be discerned with Cr. The preservation of integrity of membranes in Cr-treated cells was most probably aided by high proportion of the de-epoxidized xanthophylls, which were absent with Cd. It can thus be concluded that beside strong similarities of the toxic effects of Cr and Cd, the response of the photosynthetic machinery of C. variabilis to these two trace metal ions substantially differ from each other—strongly suggesting different inhibitory and protective mechanisms following the primary toxic events.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 73%

Similarities and Differences in the Effects of Toxic Concentrations of Cadmium and Chromium on the Structure and Functions of Thylakoid Membranes in Chlorella variabilis

et al. 2020

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“…The magnitudes of the ratios I 1 /D 2 and I 4 /D 2 characterize the overall capacity to develop the electric potential and pH differences across the thylakoid membrane, respectively (25). Based on the results of the inhibitory analysis and Monte‐Carlo simulations, we recently proposed that the ratio I 2 /I 1 reflects electron flow through the PQ pool (23). The selected parameters are related to the functional state of PSII (parameters φ Po , ψ Eo , and φ Eo ), PSI and PC ( V ox ), and intersystem electron transport together with PSI or/and PSII ( δ Ro , φ Ro , V red , I 1 /D 2 , I 2 /I 1 , I 4 /D 2 ) (Table 1).…”

Section: Methodsmentioning

confidence: 99%

Multiple in vivo Effects of Cadmium on Photosynthetic Electron Transport in Pea Plants

Todorenko

Волгушева

Тимофеев

et al. 2021

Photochem & Photobiology

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The inhibitory effects of cadmium (CdSO 4 ) on the primary photosynthetic processes were studied in vivo in Pisum sativum by using Multi-function Plant Efficiency Analyser (M-PEA-2). Photosynthetic parameters related to photosystem (PS) II, PS I and intersystem electron carriers were calculated from the light-induced kinetics of prompt chlorophyll a fluorescence (OJIP transient), delayed chlorophyll a fluorescence (DF), and 820 nm modulated reflection (MR). Lowdose exposure to cadmium (20 lM CdSO 4 for 48 h) reduced probability of electron transfer from plastoquinones to the terminal electron acceptors of PSI (d Ro ) accompanied by a decrease in the rate of P 700 + and PC reduction (V red ) and the magnitude of the I 2 step on the DF kinetics. Electron transport through PSI remained unaltered. The obtained results allowed us to propose existence of the potential site of inhibition of photosynthetic electron flow by cadmium between PSII and PSI. We propose to use parameters d Ro , V red , and I 2 /I 1 as sensitive indicators of an early contamination by heavy metals.

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“…The excessive accumulation of Cr (VI) in the soil causes enormous problems for plant growth and crop productivity [ 6 ]. A higher intake of Cr (VI) slows down seedling development, germination process, and root growth [ 7 – 9 ]. The interference of Cr (VI) with nutrient uptake, such as phosphorus, within the intracellular membrane structures and photosynthesis, increases plant phytotoxicity.…”

Section: Introductionmentioning

confidence: 99%

“…The interference of Cr (VI) with nutrient uptake, such as phosphorus, within the intracellular membrane structures and photosynthesis, increases plant phytotoxicity. This is due to lipid peroxidation through reactive oxygen species (ROS) and modification of antioxidant activities [ 9 , 10 ]. Cr crossing the plasma membrane oxidizes proteins and nucleic acid through the production of reactive oxygen species (ROS) due to its strong oxidizing nature, such as radicals, O 2− , OH − , and H 2 O 2 [ 7 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Improved chromium tolerance of Medicago sativa by plant growth-promoting rhizobacteria (PGPR)

Tirry

Kouchou

Omari

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Soil pollution by heavy metals increases the bioavailability of metals like hexavalent chromium (Cr (VI)), subsequently limiting plant growth and reducing the efficiency of phytoremediation. Plant growth-promoting rhizobacteria (PGPR) have substantial potential to enhance plant growth as well as plant tolerance to metal stress. The aim of this research was to investigate Cr (VI) phytoremediation enhancement by PGPR. Results The results showed that the 27 rhizobacterial isolates studied were confirmed as Cr (VI)-resistant PGPR, by using classical biochemical tests (phosphate solubilization, nitrogen fixation, indole acetic acid, exopolysaccharides, hydrogen cyanide, siderophores, ammonia, cellulase, pectinase, and chitinase production) and showed variable levels of Cr (VI) resistance (300–600 mg/L). The best four selected Cr (VI)-resistant PGPR (NT15, NT19, NT20, and NT27) retained most of the PGP traits in the presence of 100–200 mg/L concentrations of Cr (VI). The inoculation of Medicago sativa with any of these four isolates improved the shoot and root dry weight. The NT27 isolate identified using 16S rDNA gene sequence analyses as a strain of Pseudomonas sp. was most effective in terms of plant growth promotion and stress level decrease. It increased shoot and root dry weights of M. sativa by 97.6 and 95.4%, respectively, in the presence of Cr (VI) when compared to non-inoculated control plants. It also greatly increased chlorophyll content and decreased the levels of stress markers, malondialdehyde, hydrogen peroxide, and proline. The results of the effect of Pseudomonas sp. on Cr content and bioaccumulation factor (BAF) of the shoots and roots of M. sativa plants showed the increase of plant biomass concomitantly with the increase of Cr root concentration in inoculated plants. This would lead to a higher potential of Cr (VI) phytostabilization. Conclusions This study demonstrates that the association M. sativa-Pseudomonas sp. may be an efficient biological system for the bioremediation of Cr (VI)-contaminated soils.

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Chromium effects on photosynthetic electron transport in pea (Pisum sativum L.)

Cited by 29 publications

References 60 publications

Similarities and Differences in the Effects of Toxic Concentrations of Cadmium and Chromium on the Structure and Functions of Thylakoid Membranes in Chlorella variabilis

Similarities and Differences in the Effects of Toxic Concentrations of Cadmium and Chromium on the Structure and Functions of Thylakoid Membranes in Chlorella variabilis

Multiple in vivo Effects of Cadmium on Photosynthetic Electron Transport in Pea Plants

Improved chromium tolerance of Medicago sativa by plant growth-promoting rhizobacteria (PGPR)

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