Chromobacterium, Eikenella, Kingella, Neisseria, Simonsiella, and Vitreoscilla Species Comprise a Major Branch of the Beta Group Proteobacteria by 16S Ribosomal Ribonucleic Acid Sequence Comparison: Transfer of Eikenella and Simonsiella to the Family Neisseriaceae (emend.)

Uruburuella suis gen. nov., sp. nov., isolated from clinical specimens of pigs

“…Phylogenetically, the family encompasses the genera Neisseria, Kingella, Eikenella, Simonsiella and Alysiella (Dewhirst et al, 1989;Rossau et al, 1989). It is clear from the results of the present study that the unidentified bacterium from pigs is phylogenetically a member of this family grouping.…”

“…Subsequent studies by Rossau et al (1989) showed that the genera Neisseria, Kingella, Eikenella, Simonsiella and Alysiella were closely related and should be classified together in the family Neisseriaceae, which comprises a major branch of the b-Proteobacteria. Comparative 16S rRNA gene sequencing studies by Dewhirst et al (1989) confirmed the results of Rossau et al (1989) and led to the recommendation that the genera Eikenella and Simonsiella be transferred to the family Neisseriaceae. In recent years, other genera, e.g.…”

“…It is pertinent to note that the five isolates possessed an adenine and a uracil at positions 585 and 756, respectively (numbering relative to the Escherichia coli sequence), in their 16S rRNA. These signatures are characteristic of members of the family Neisseriaceae (Dewhirst et al, 1989). Treeing analysis confirmed this affinity, and the unknown bacterium (as exemplified by strain 1258/02 T ) formed a distinct subline within a large rRNA cluster that included the genera Neisseria, Kingella, Alysiella, Eikenella and Simonsiella.…”

“…The family Neisseriaceae has undergone various revisions over the years, and numerous other genera have been assigned to it [e.g. Psychrobacter (Juni & Heym, 1986), Mesophilobacter (Nishimura et al, 1989), Alysiella (Rossau et al, 1989) and Eikenella and Simonsiella (Dewhirst et al, 1989)]. On the basis of rRNA cistron similarity studies, Rossau et al (1986) recommended that the genera Acinetobacter, Moraxella and Branhamella be excluded from the family Neisseriaceae.…”

Phylogeny of the genus Simonsiella and other members of the Neisseriaceae.

“…Steed (1962) designated the family Simonsiellaceae to include Simonsiella and the superficially similar genus Alysiella ; however, the genus Alysiella has been reported to be unrelated to Simonsiella (Stackebrandt et al, 1988). Dewhirst et al (1989) sequenced 16S rRNA from the type strain of S. muelleri and found that it clustered within the Neisseriaceae in the β-Proteobacteria. Appropriately, the authors emended the family Neisseriaceae to include Simonsiella.…”