Chromoblastomycosis Caused by Fonsecaea nubica: First Report in Northern China and Literature Review

You, Zimeng; Yang, Xin; Jiang-an, Zhang; Ran, Yuping

doi:10.1007/s11046-018-0307-0

Cited by 8 publications

(7 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…F. nubica is a genetic sibling of F. monophora. So far, there are more than 40 strains of F. nubica reported in published literature from Brazil and China (Gomes et al, 2016;Fransisca et al, 2017;You et al, 2019). Surprisingly, we did not isolate F. pedrosoi in the Guangdong region.…”

Section: Discussioncontrasting

confidence: 62%

Molecular Characteristics of Regional Chromoblastomycosis in Guangdong, China: Epidemiological, Clinical, Antifungal Susceptibility, and Serum Cytokine Profiles of 45 Cases

Liu

Sun

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Chromoblastomycosis (CBM) is a chronic disease caused by several species of dematiaceous fungi. In this study, a regional collection of 45 CBM cases was conducted in Guangdong, China, a hyper-endemic area of CBM. Epidemiology findings indicated that the mean age of cases was 61.38 ± 11.20 years, long duration ranged from 3 months to 30 years, and the gender ratio of male to female was 4.6:1. Thirteen cases (29%) declared underlying diseases. Verrucous form was the most common clinical manifestation (n = 19, 42%). Forty-five corresponding clinical strains were isolated, and 28 of them (62%) were identified as F. monophora; the remaining 17 (38%) were identified as F. nubica through ITS rDNA sequence analysis. Antifungal susceptibility tests in vitro showed low MICs in azoles (PCZ 0.015–0.25 μg/ml, VCZ 0.015–0.5 μg/ml, and ITZ 0.03–0.5 μg/ml) and TRB (0.015–1 μg/ml). Itraconazole combined with terbinafine was the main therapeutic strategy used for 31 of 45 cases, and 68% (n = 21) of them improved or were cured. Cytokine profile assays indicated upregulation of IL-4, IL-7, IL-15, IL-11, and IL-17, while downregulation of IL-1RA, MIP-1β, IL-8, and IL-16 compared to healthy donors (p < 0.05). The abnormal cytokine profiles indicated impaired immune response to eliminate fungus in CBM cases, which probably contributed to the chronic duration of this disease. In conclusion, we investigated the molecular epidemiological, clinical, and laboratory characteristics of CBM in Guangdong, China, which may assist further clinical therapy, as well as fundamental pathogenesis studies of CBM.

show abstract

Section: Discussioncontrasting

confidence: 62%

Molecular Characteristics of Regional Chromoblastomycosis in Guangdong, China: Epidemiological, Clinical, Antifungal Susceptibility, and Serum Cytokine Profiles of 45 Cases

Liu

Sun

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…These limitations may be overcome by the sequencing of other loci and/or by using other molecular methods. The combination of CDC42 , ACT1 , BT2 , lactase ( Lac ), homogentisate (HmgA), and polyketide synthase ( PKS1 ) (the first three genes are the most common) with ITS sequencing can increase the accuracy [ 17 ]. Based on our study, F .…”

Section: Discussionmentioning

confidence: 99%

Chromoblastomycosis: A case series from Eastern China

et al. 2022

View full text Add to dashboard Cite

Chromoblastomycosis (CBM) is a chronic fungal infection of the cutaneous and subcutaneous tissues caused by brown pigmented fungi. Fonsecaea monophora is one of the most common pathogens of CBM in China. Most formal cases have been reported from Southern China, however, the infection is not uncommon in Eastern China where very few case series are available. To describe the clinical aspects of CBM, we report a series of 11 cases between 2018 and 2021 at a single medical center in Eastern China. The patients were predominately male (n = 9) and the disease duration ranged from 3 months to 20 years. Plaque type lesions were the most common clinical manifestations. There were 7 cases of mild forms and 3 cases of severe forms. Among the 3 severe cases, one case gave up treatment due to economic poverty; one case did not respond to a 1-year systemic treatmen; one case was cured by combination therapy of 10 months. Other cases were cured by treatment with antifungal agents. All cases of direct mycological examination were positive. All isolates were identified by morphology and sequencing of the the ITS regions of ribosomal DNA, Ten were F. monophora and 1 was Cladophialophora carrionii. All cases had been evaluated at other clinics, where 8 cases were misdiagnosed as other diseases. As a neglected tropical disease (NTD), CBM is still a major challenge in the field of dermatology, especially in its severe clinical forms. As an effective and simple diagnostic method of CBM, direct microscopic examination should be further promoted in rural hospitals.

show abstract

“…In the present work, we described and characterized for the first time EVs produced by F. pedrosoi , F. nubica , and F. erecta . F. pedrosoi and F. nubica species are etiologic agents of chromoblastomycosis (CBM), whereas F. erecta is an environmental fungus ( Najafzadeh et al., 2010 ; Vicente et al., 2014 ; Queiroz-Telles et al., 2017 ; You et al., 2019 ). Fungal EVs have a role not only in fungal metabolism but also in fungal pathogenicity and survival inside the host ( Rodrigues et al., 2008 ; Bielska et al., 2018 ; Ikeda et al., 2018 ; Zhao et al., 2019 ; Rizzo et al., 2020a ).…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity and Growth Conditions Modulate Fonsecaea Extracellular Vesicles’ Ability to Interact With Macrophages

Las-Casas

Marina

Castro

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Chromoblastomycosis (CBM) is a chronic cutaneous and subcutaneous mycosis caused by black, dimorphic, and filamentous fungi of the Herpothrichiellaceae family, such as species of the genus Fonsecaea. These fungi can switch between the saprophytic forms (conidia and hyphae) and the pathogenic form, the muriform cells (MCs), which is considered an essential mechanism for fungal virulence. Nearly all types of cells can produce membranous structures formed by a lipid bilayer that communicate extracellularly with other cells, known as “extracellular vesicles” (EVs), which may act as virulence factors, as observed for several species of pathogenic fungi. Our findings demonstrated for the first time that F. pedrosoi, F. nubica, and F. erecta produce EVs in response to nutritional conditions. The EVs varied in sterol and protein contents, size, and morphology. Moreover, the EVs induced different cytokine and nitric oxide release patterns by bone marrow-derived macrophages (BMDMs). The EVs activated IL-1β production, possibly acting as the first signal in inflammasome activation. Unlike the pathogenic species, the EVs isolated from F. erecta did not significantly stimulate TNF and IL-10 production in general. Overall, these results demonstrated that different species of Fonsecaea produce EVs capable of modulating pro- and anti-inflammatory cytokine and nitric oxide production by BMDMs and that growth conditions affected the immunomodulatory capacities of the EVs as well as their size, content, and morphology.

show abstract

Chromoblastomycosis Caused by Fonsecaea nubica: First Report in Northern China and Literature Review

Cited by 8 publications

References 32 publications

Molecular Characteristics of Regional Chromoblastomycosis in Guangdong, China: Epidemiological, Clinical, Antifungal Susceptibility, and Serum Cytokine Profiles of 45 Cases

Molecular Characteristics of Regional Chromoblastomycosis in Guangdong, China: Epidemiological, Clinical, Antifungal Susceptibility, and Serum Cytokine Profiles of 45 Cases

Chromoblastomycosis: A case series from Eastern China

Pathogenicity and Growth Conditions Modulate Fonsecaea Extracellular Vesicles’ Ability to Interact With Macrophages

Contact Info

Product

Resources

About