Chromogranins can be measured in samples from cats and dogs

Stridsberg, Mats; Pettersson, Ann; Hagman, Ragnvi; Westin, Christoffer; Höglund, Odd Viking

doi:10.1186/1756-0500-7-336

Cited by 15 publications

(26 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Competitive radioimmunoassay (RIA) was used for measuring CST and VS. All heparinized plasma samples were analyzed in duplicates at the Clinical Chemistry Laboratory, Uppsala University Hospital, Uppsala, Sweden as previously described [4, 70]. This method has been developed for both tissue and circulation and used for measuring CgA in humans.…”

Section: Methodsmentioning

confidence: 99%

“…An active secretion of CgA into saliva in the submandibular gland has been found in humans [9], horses, and rats [10, 11]. Chromogranin A can be measured in saliva and blood in humans, pigs, cows, and dogs [4, 12–15]. Several bioactive peptides are derived from CgA degradation, including vasostatin, pancreastatin, catestatin, and serpinin [16–33].…”

Section: Introductionmentioning

confidence: 99%

“…Even though there are interspecies differences in CgA amino acid sequences, CgA can be measured in dogs [4]. A study on cross-reactivity between humans and dogs against different regions of the CgA molecule showed that CgA 17–38 (vasostatin) and CgA 361–372 (catestatin) could be measured using competitive radioimmunoassay (RIA) in dogs, whereas intact CgA could not [4].…”

Section: Introductionmentioning

confidence: 99%

“…A study on cross-reactivity between humans and dogs against different regions of the CgA molecule showed that CgA 17–38 (vasostatin) and CgA 361–372 (catestatin) could be measured using competitive radioimmunoassay (RIA) in dogs, whereas intact CgA could not [4]. Catestatin (CST) and vasostatin (VS), both CgA derived peptides, are bioactive.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Catestatin and vasostatin concentrations in healthy dogs

et al. 2017

Self Cite

View full text Add to dashboard Cite

BackgroundThe neuroendocrine glycoprotein chromogranin A is a useful biomarker in humans for neuroendocrine tumors and stress. Chromogranin A can be measured in both blood and saliva. The objective of this study was to investigate concentrations of and correlation between the chromogranin A epitopes catestatin and vasostatin in healthy dogs accustomed to the sample collection procedures. Blood and saliva samples were collected from 10 research Beagle dogs twice daily for 5 consecutive days, and from 33 privately-owned blood donor dogs in association with 50 different blood donation occasions. All dogs were familiar with sample collection procedures. During each sampling, stress behavior was scored by the same observer using a visual analog scale (VAS) and serum cortisol concentrations. Catestatin and vasostatin were analyzed using radioimmunoassays for dogs.ResultsThe dogs showed minimal stress behavior during both saliva sampling and blood sampling as monitored by VAS scores and serum cortisol concentrations. Few and insufficient saliva volumes were obtained and therefore only catestatin could be analyzed. Catestatin concentrations differed significantly and did not correlate significantly with vasostatin concentrations (P < 0.0001). Age, gender, breed, and time of sample collection did not significantly affect concentrations of plasma catestatin, vasostatin, and saliva catestatin.ConclusionsThe normal ranges of plasma catestatin (0.53–0.98 nmol/l), vasostatin (0.11–1.30 nmol/l), and saliva catestatin (0.31–1.03 nmol/l) concentrations in healthy dogs accustomed to the sampling procedures were determined. Separate interpretation of the different chromogranin A epitopes from either saliva or plasma is recommended.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Catestatin and vasostatin concentrations in healthy dogs

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Samples were analyzed in duplicate by competitive RIA at the Clinical Chemistry Laboratory, Uppsala University Hospital, Uppsala, Sweden as earlier described [43]. The overall coefficient of variation (CV) in this study was <10%.…”

Section: Methodsmentioning

confidence: 99%

Catestatin, vasostatin, cortisol, and pain assessments in dogs suffering from traumatic bone fractures

et al. 2017

Self Cite

View full text Add to dashboard Cite

BackgroundTraumatic bone fractures cause moderate to severe pain, which needs to be minimized for optimal recovery and animal welfare, illustrating the need for reliable objective pain biomarkers for use in a clinical setting. The objectives of this study were to investigate catestatin (CST) and vasostatin (VS) concentrations as two new potential biomarkers, and cortisol concentrations, scores of the short form of the Glasgow composite measure pain scale (CMPS-SF), and visual analog scale (VAS) in dogs suffering from traumatic bone fractures before and after morphine administration in comparison with healthy dogs.MethodsFourteen dogs with hind limb or pelvic fractures and thirty healthy dogs were included. Dogs with fractures were divided into four groups according to analgesia received before participation. Physical examination, CMPS-SF, pain and stress behavior VAS scores were recorded in all dogs. Saliva and blood were collected once in healthy dogs and in dogs with fractures before and 35–70 min after morphine administration. Blood samples were analyzed for CST, VS, and cortisol. Saliva volumes, however, were insufficient for analysis.ResultsCatestatin and cortisol concentrations, and CMPS-SF, and VAS scores differed significantly between dogs with fractures prior to morphine administration and healthy dogs. After morphine administration, dogs with fractures had significantly decreased CMPS-SF and VAS scores and, compared to healthy dogs, CST concentrations, CMPS-SF, and VAS scores still differed significantly. However, CST concentrations remained largely within the normal range. Absolute delta values for CST significantly correlated with delta values for CMPS-SF. Catestatin and cortisol did not differ significantly before and after morphine administration. Vasostatin concentrations did not differ significantly between groups.ConclusionsCatestatin and cortisol concentrations, CMPS-SF, and VAS scores differed significantly in the dogs with traumatic bone fractures compared to the healthy dogs. Morphine treatment partially relieved pain and stress according to the subjective but not according to the objective assessments performed. However, because of the large degree of overlap with normal values, our results suggest that plasma CST concentrations have a limited potential as a clinically useful biomarker for pain-induced stress.

show abstract

Biosynthesis, intracellular-trafficking, and exocytosis of insulin granules

Gupta

2022

Understanding Insulin and Insulin Resistance

View full text Add to dashboard Cite

Chromogranins can be measured in samples from cats and dogs

Cited by 15 publications

References 18 publications

Catestatin and vasostatin concentrations in healthy dogs

Catestatin and vasostatin concentrations in healthy dogs

Catestatin, vasostatin, cortisol, and pain assessments in dogs suffering from traumatic bone fractures

Biosynthesis, intracellular-trafficking, and exocytosis of insulin granules

Contact Info

Product

Resources

About