Chromophore-protein interaction controls the complexity of the phytochrome photocycle

Schmidt, P.; Westphal, Ulf H; Worm, Karl; Braslavsky, Silvia E.; Gärtner, Wolfgang; Schaffner, Kurt

doi:10.1016/1011-1344(95)07269-1

Cited by 39 publications

(47 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1), although the spectrum of the PCB-adduct is blue-shifted. Since a similar 10-nm blue shift is observed when PCB is substituted in place of PDB for in vitro assembly with recombinant oat apophytochrome A (22,25), the spectral data shown in Fig. 4 strongly supports the hypothesis that the Pichia pigment is PDB.…”

Section: Expression Of Algal Phytochrome Yields Photochromicsupporting

confidence: 67%

The methylotrophic yeast Pichia pastoris synthesizes a functionally active chromophore precursor of the plant photoreceptor phytochrome.

Lagarias

1996

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Induction of the expression of an algal phytochrome cDNA in the methylotrophic yeast Pichia pastoris led to time-dependent formation of photoactive holophytochrome without the addition of exogenous bilins. Both in vivo and in vitro difference spectra of this photochromic species are very similar to those of higher plant phytochrome A, supporting the conclusion that this species possesses a phytochromobilin prosthetic group. Zinc blot analyses confirm that a bilin chromophore is covalently bound to the algal phytochrome apoprotein. The hypothesis that P. pastoris contains phytochromobilin synthase, the enzyme that converts biliverdin IXa to phytochromobilin, was also addressed in this study. Soluble extracts from P. pastoris were able to convert biliverdin to a bilin pigment, which produced a native difference spectrum upon assembly with oat apophytochrome A. HPLC analyses confirm that biliverdin is converted to both 3E-and 3Z-isomers of phytochromobilin. These investigations demonstrate that the ability to synthesize phytochromobilin is not restricted to photosynthetic organisms and support the hypothesis of a more widespread distribution of the phytochrome photoreceptor.

show abstract

Section: Expression Of Algal Phytochrome Yields Photochromicsupporting

confidence: 67%

The methylotrophic yeast Pichia pastoris synthesizes a functionally active chromophore precursor of the plant photoreceptor phytochrome.

Lagarias

1996

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Such a blue shift is also observed when higher-plant phytochrome autoassembles with PCB. Reported difference maxima for such adducts are at 650-658 and 709-720 nm (11,(34)(35)(36)(37)(38)(39)(40)(41); the equivalent values for our preparations are 655 and 708 nm. Conversely, the difference spectrum of our P⌽B adduct is similar to that of oat PHYA (Fig.…”

Section: Discussionmentioning

confidence: 99%

Characterization of recombinant phytochrome from the cyanobacterium Synechocystis

Lamparter

Mittmann

Gärtner

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

173

223

View full text Add to dashboard Cite

following correction should be noted. Due to an editorial change at PNAS, the meaning of the last sentence on page 14046 was altered. The sentence originally read as follows: On the other hand, this structure does not reproduce the pharmacological properties of either P or Q channel exactly, as the ID 50 to sFTX and -Aga IVA for P-type channels is lower than for the ␣1A, ␣2␦, ␤Ib channels in HEK cells.Neurobiology. In the article "The synthesis of ATP by glycolytic enzymes in the postsynaptic density and the effect of endogenously generated nitric oxide" Kuo Wu, Chiye Aoki, Alice Elste, Adrienne A. Rogalski-Wilk, and Philip Siekevitz, which appeared in number 24, November 25, 1997, of Proc. Natl. Acad. Sci. USA (94,(13273)(13274)(13275)(13276)(13277)(13278), the quality of the reproduction of Fig. 2A was poor. The figure and its legend are shown below:Biochemistry. In the article "KSR stimulates Raf-1 activity in a kinase-independent manner" by Neil R. Michaud, Marc Therrien, Angela Cacace, Lisa C. Edsall, Sarah Spiegel, Gerald M. Rubin, and Deborah K. Morrison, which appeared in number 24, November 25, 1997, of Proc. Natl. Acad. Sci. USA (94,(12792)(12793)(12794)(12795)(12796), the following correction should be noted.Due to a printer's error, background was incorrectly added to (50 g) and 100 g each of the other fractions, in 100 l final volume, including whole homogenate (H), synaptosomes (Syn), synaptic plasma membranes (SPM), and crude synaptic vesicles (CSV), were incubated at 37°C for 15 min. NAD incorporation was performed in the absence (Ϫ) or presence (ϩ) of SNP as exogenous source of NO. The mixtures were subjected to SDS͞PAGE and then autoradiography. (B) Western blot analysis of the G3PD in the subcellular fractions. To confirm that the radioactive protein in the subcellular fractions was indeed G3PD, Western blot analysis was performed by using specific anti-G3PD antibodies as described.

show abstract

“…34 Moreover, the photophysical and photochemical properties of wild-type phytochrome are quite similar to those of the reconstituted chromoproteins. 35,36 Other strategies, including systematic N-and C-terminal truncations and site-directed mutagenesis of the apoprotein, have been used to study the structural requirements of the chromophore-apoprotein interaction in terms of photochromism. [37][38][39] Even though such bilin chromophores could be isolated from natural sources, knowledge of the relationship between the structure of synthetic bile pigments and the biochemical properties of the reconstituted biliproteins prepared by combining them with an apoprotein is quite interesting and important to determine the precise function of the bilin chromophores.…”

Section: Introductionmentioning

confidence: 99%

Studies on the Structure and Function of Phytochromes as Photoreceptors Based on Synthetic Organic Chemistry

Inomata

2008

Bulletin of the Chemical Society of Japan

View full text Add to dashboard Cite

show abstract

Chromophore-protein interaction controls the complexity of the phytochrome photocycle

Cited by 39 publications

References 14 publications

The methylotrophic yeast Pichia pastoris synthesizes a functionally active chromophore precursor of the plant photoreceptor phytochrome.

The methylotrophic yeast Pichia pastoris synthesizes a functionally active chromophore precursor of the plant photoreceptor phytochrome.

Characterization of recombinant phytochrome from the cyanobacterium Synechocystis

Studies on the Structure and Function of Phytochromes as Photoreceptors Based on Synthetic Organic Chemistry

Contact Info

Product

Resources

About