2018
DOI: 10.1021/jacs.8b04659
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Chromophore–Protein Interplay during the Phytochrome Photocycle Revealed by Step-Scan FTIR Spectroscopy

Abstract: Phytochrome proteins regulate many photoresponses of plants and microorganisms. Light absorption causes isomerization of the biliverdin chromophore, which triggers a series of structural changes to activate the signaling domains of the protein. However, the structural changes are elusive, and therefore the molecular mechanism of signal transduction remains poorly understood. Here, we apply two-color step-scan infrared spectroscopy to the bacteriophytochrome from Deinococcus radiodurans. We show by recordings i… Show more

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Cited by 64 publications
(107 citation statements)
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“…The lifetimes of the remaining SADS components, corresponding to the subsequent states in the photocycle, are in good agreement with the earlier reports. 6,16,28 We confirm that these three SADS represent the transitions between Lumi-R, Meta-R and Pfr states, respectively. The newly resolved extra state has spectral features similar to those of the Lumi-R state except the characteristic chromophore absorption above 1700 cm À1 .…”
Section: The Photocycle Routes In Wt and Y263f Cbd-phy Revealed By Nssupporting
confidence: 73%
See 4 more Smart Citations
“…The lifetimes of the remaining SADS components, corresponding to the subsequent states in the photocycle, are in good agreement with the earlier reports. 6,16,28 We confirm that these three SADS represent the transitions between Lumi-R, Meta-R and Pfr states, respectively. The newly resolved extra state has spectral features similar to those of the Lumi-R state except the characteristic chromophore absorption above 1700 cm À1 .…”
Section: The Photocycle Routes In Wt and Y263f Cbd-phy Revealed By Nssupporting
confidence: 73%
“…The SADS corresponding to the Pfr state ( Fig. 5A) matches closely with the steady-state FTIR difference spectrum (Pfr minus Pr, 28 and Fig. S3, ESI †), which indicates that the complete photocycle has been resolved in the time window of the experiment.…”
Section: The Photocycle Routes In Wt and Y263f Cbd-phy Revealed By Nssupporting
confidence: 64%
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