Chromoplast biogenesis and carotenoid accumulation

Li, Li; Yuan, Hui

doi:10.1016/j.abb.2013.07.002

Cited by 220 publications

(203 citation statements)

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“…Considerable progress has been made in the understanding of chromoplast differentiation in the pericarp of citrus fruits (Eilati et al, 1969;Iglesias et al, 2007), which is a process similar to that of tomato and pepper (Egea et al, 2010). However, little is known about the molecular basis of chromoplast differentiation in the edible flesh, even though there is increasing evidence suggesting an essential role of carotenoid synthesis in inducing chromoplast differentiation (Egea et al, 2010;Bian et al, 2011;Li and Yuan, 2013). Recently, we successfully isolated and purified intact chromoplasts containing a large number of plastoglobules from the flesh of sweet orange (Citrus sinensis) fruits at the maturation stage (Zeng et al, 2011).…”

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confidence: 99%

“…In some cases, chromoplasts also arise from nonphotosynthetic plastids, such as colorless proplastids, leucoplasts, or amyloplasts (Knoth et al, 1986;Schweiggert et al, 2011). To date, most studies on chromoplast differentiation have been focused on the synthesis of carotenoids by combining biochemical and molecular analyses (Cazzonelli and Pogson, 2010;Egea et al, 2010;Bian et al, 2011;Li and Yuan, 2013), and little is known about the molecular mechanisms underlying chromoplast biogenesis .…”

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confidence: 99%

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A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh

Zeng

Wang

et al. 2015

Plant Physiol.

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(S.X.).Globular and crystalloid chromoplasts were observed to be region specifically formed in sweet orange (Citrus sinensis) flesh and converted from amyloplasts during fruit maturation, which was associated with the composition of specific carotenoids and the expression of carotenogenic genes. Subsequent isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analyses of purified plastids from the flesh during chromoplast differentiation and senescence identified 1,386 putative plastid-localized proteins, 1,016 of which were quantified by spectral counting. The iTRAQ values reflecting the expression abundance of three identified proteins were validated by immunoblotting. Based on iTRAQ data, chromoplastogenesis appeared to be associated with three major protein expression patterns: (1) marked decrease in abundance of the proteins participating in the translation machinery through ribosome assembly; (2) increase in abundance of the proteins involved in terpenoid biosynthesis (including carotenoids), stress responses (redox, ascorbate, and glutathione), and development; and (3) maintenance of the proteins for signaling and DNA and RNA. Interestingly, a strong increase in abundance of several plastoglobule-localized proteins coincided with the formation of plastoglobules in the chromoplast. The proteomic data also showed that stable functioning of protein import, suppression of ribosome assembly, and accumulation of chromoplast proteases are correlated with the amyloplast-to-chromoplast transition; thus, these processes may play a collective role in chromoplast biogenesis and differentiation. By contrast, the chromoplast senescence process was inferred to be associated with significant increases in stress response and energy supply. In conclusion, this comprehensive proteomic study identified many potentially new plastid-localized proteins and provides insights into the potential developmental and molecular mechanisms underlying chromoplast biogenesis, differentiation, and senescence in sweet orange flesh.

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confidence: 99%

A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh

Zeng

Wang

et al. 2015

Plant Physiol.

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“…Indeed, plastids found in roots such as proplastids or leucoplasts are not specialized in carotenoid accumulation (Howitt and Pogson, 2006;Li and Yuan, 2013). Therefore, this shortage of carotenoids could limit the ability of roots to supply high levels of ABA to leaves where actually large amounts of the phytohormone are detected under water stress (Cornish and Zeevaart, 1985;Correia et al, 2014).…”

Section: Aba Transportmentioning

confidence: 99%

“…Furthermore, carotenoids are synthesized in several plant tissues; however, their accumulation depends on the plastid ability to sequester these pigments in defined sink structures (Li and Yuan 2013). In roots the main plastids are proplastids and leucoplasts which, in contrast to chloroplast and chromoplasts (primarily found in leaves and fruits), are not specialized in carotenoid accumulation (Howitt andPogson 2006, Li andYuan 2013).…”

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confidence: 99%

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Involvement of aerial organs on the ABA accumulation in roots of Citrus plants under water deficit

Fraga¹,

Jesús²

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HACEN CONSTAR QUE:Matías Jesús Manzi Fraga, Ingeniero Agrónomo por la Universidad de la República de Uruguay y Máster en Técnicas Cromatográficas Aplicadas por la Universitat Jaume I, ha realizado la bajo su dirección el trabajo que, titulado 'Involvement of aerial organs on the ABA accumulation in roots of Citrus plants under water deficit' reúne las condiciones necesarias para optar al grado de Doctor. Dr. Aurelio Gómez CadenasDr. Vicent Arbona Mengual Castellón de la Plana, Mayo 2016 AGRADECIMIENTOSAcá sí que me tengo a esmerar ya que conozco a unos cuantos (vagos) que solamente van a leer esta sección, incluso hay varios que están esperando a que salga la película para no leer!!! Se imaginarán que son muchas las personas a las que agradecer, no solo por la tesis sino también para que la tremenda experiencia por tierras levantinas. No voy a especificar los motivos porque sino no termino nunca, pero estoy seguro que cada uno sabe lo agradecido que estoy.En primera instancia, a mis directores, Aurelio y Vicent, que me permitieron realizar ésta tesis. Sin lugar a dudas, el aprendizaje ha sido tremendo. Cada experiencia ayuda a crecer y por lo tanto no tengo más que palabras de agradecimiento.Por supuesto que a Rosa, gran flechadora de canchas si las hay!!! Que a més a més, no sólo no me va a complicar la vida en la presentación, sino que me aguantó durante cientos de kms. Lo quiero dejar bien clarito acá, no solo es una excelente conductora y mi vida nunca estuvo en riesgo, sino que además no tiene un pelo de chirusa. Moltes gracies Rose! A mis compas de labo: Tico, Sara, María, Marta, Alejandro, Vale, que lo hemos pasado bien de bien durante todo este tiempo "hashiendo coshash". No me alcanzan las palabras para agradecerles por tantas y tantas manos. Así que adapté una frase célebre para decirles que: "es Matías el que elige a los amigos y son los amigos los que quieren que sea Matías el amigo. Muchash grashiash!!!" (Fin de la cita).Además, recordar en la UJI a Elvira y Pilar con los papeles y las plantas, a Carlos y Jorge del labo, a los vecinos, Merche, Raúl, Loredana, Emma, Victoria y por supuesto a Table S1. ABA, β,β-carotenoid and total carotenoid content in shoots of CT plants in response to PEG………………………………………………………………………………………..………………………… 83 Table S2. Sequences of the primers used for qRT-PCR………………………………………………… 84Chapter III Supporting information Table S1. Globally, this thesis provides evidence that roots of Citrus plants are not an important source of ABA under water deficit and conversely, leaves sustain the accumulation of ABA in those dehydrated roots. Furthermore, data present here suggest that leaves are key not only in providing ABA to roots but also in triggering the water stress responses which lead to the ABA accumulation in both, leaves and roots. RESUMENLa sequía es una de las situaciones más perjudiciales para el crecimiento y la supervivencia de las plantas. Sin embargo, las plantas han desarrollado diversas estrategias para afrontar dichas condiciones, incluyendo la rápida señalización...

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Biosynthesis and metabolism

Rodriguez–Amaya

2015

Food Carotenoids

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Chromoplast biogenesis and carotenoid accumulation

Cited by 220 publications

References 129 publications

A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh

A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh

Involvement of aerial organs on the ABA accumulation in roots of Citrus plants under water deficit

Biosynthesis and metabolism

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