Chromosomal Aberration Test in Human Lymphocytes

Johannes, Christian; Obe, G.

doi:10.1007/978-1-4939-9646-9_6

Cited by 2 publications

(2 citation statements)

References 38 publications

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“…Very high correspondence was found between the results obtained in the chromosomal aberration test and the comet assay using human peripheral lymphocytes and Chinese hamster lung cells treated with methyl methanesulfonate, cyclophosphamide, and novel pharmaceuticals. However, positive results in the comet assay were always observed at higher doses than those that produced an effect in the cytogenetic test [29]. The results of this study are also in line with another study which found that the pathogenicity of Al, Cu, Cr, Ni, Zn, Fe, and Pb caused more DNA damage to peripheral red blood cells (RBCs) of the domestic fish species Hyphssobryconluetkenii [30].…”

Section: Discussionsupporting

confidence: 90%

Detection of DNA Damage in Fish using Comet Assay

Chatha,

Naz,

Iqbal

et al. 2023

CTO

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Heavy metals have an enduring presence, risky characteristics, and the propensity to accumulate in the environment. This is why heavy metal toxics are widely acknowledged as harmful environmental pollutants. Heavy metals damage both aquatic and terrestrial ecosystems, posing a major risk to the environment and human health. Four freshwater fish species namely Labeo rohita, Catla catla, Hypophthalmichthys molitrix, and Ctenopharyngodon idella were the focus of this investigation. This study investigated the potential genotoxic effects of lead (Pb), copper (Cu), and cadmium (Cd) on the above fish species through the application of comet assay test. The fish were exposed to these metals at four distinct concentrations (19%, 24%, 31%, and 50% of the LC50) over the course of 40 days. All four fish species were exposed to metals to varying degrees, according to the genetic damage index, cumulative tail length of comets, and the proportion of damaged cells. In contrast to Catla catla, Hypophthalmichthys molitrix had the highest prevalence of DNA damage. The current study suggests that the presence of these particular metals in Pakistan's aquatic ecosystems may have an adverse effect on the DNA of the country's fish species. Metals cause damage to DNA in fibroblast cells through distinct mechanisms when present in water, air, and soil. Comet assay test has a remarkable sensitivity that helps to identify extremely low amounts of DNA damage. Out of the four fish species, Ctenopharyngodon idella showed higher levels of damaged cells, a higher genetic damage index, and a cumulative comet tail length as compared to others. All four fish species experienced a significant increase in DNA damage, genetic damage index, and comet tail length at 50% concentration of metals LC50.

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Section: Discussionsupporting

confidence: 90%

Detection of DNA Damage in Fish using Comet Assay

Chatha,

Naz,

Iqbal

et al. 2023

CTO

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“…20,21 Genotoxicity assays in peripheral blood lymphocytes are highly useful for an initial evaluation of the side effects of chemical, physical, or biological agents. 22,23 Medicinal plants, generally lack scientific studies that determine their possible side effects. Plant species with abortive properties may pose a dual health risk due to their toxicity, potentially causing harm to both the mother and the embryo, and resulting in permanent damage.…”

Section: 15mentioning

confidence: 99%

Effect of Ambrosia tenuifolia Spreng on Danio rerio Embryo and Human Cells In Vitro

2024

TJNPR

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Ambrosia tenuifolia is a perennial herbaceous plant of the Asteraceae family, commonly used in Paraguay for multiple purposes as an abortive, antipyretic, digestive, and against headaches commonly in the form of an infusion of aerial parts. However, the lack of information on adverse effects raises questions about its safety and potential side effects. This study was undertaken to establish the potential health risks of A. tenuifolia for human health by evaluation of the aqueous and ethanolic extracts on Danio rerio embryos and to determine the potential toxicity or teratogenicity of the aqueous extract on human cells using human peripheral blood lymphocytes (PBL). For the test with D. rerio, observations were made 24 to 96 hours after fertilization. Coagulation, somite formation, heart rate, blood flow, pigmentation, edema formation, hatching, and spinal deviation were evaluated. As a result, the extract has lethal, sublethal, and teratogenic effects on the embryonic development of D. rerio, whose effects were dose-dependent. In the PBL, cells with and without bioactivation with the solution containing the S9 fraction were found to have genotoxic effects. This study provides the first report of toxicity and genotoxic effects of extracts of A. tenuifolia using D. rerio embryos and PBL after in vitro exposure.

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Chromosomal Aberration Test in Human Lymphocytes

Cited by 2 publications

References 38 publications

Detection of DNA Damage in Fish using Comet Assay

Detection of DNA Damage in Fish using Comet Assay

Effect of Ambrosia tenuifolia Spreng on Danio rerio Embryo and Human Cells In Vitro

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