Chromosomal Aberration Test Utilities In Vitro and In Vivo

Guimarães, Ana Paula Araújo; Guimarães, Adriana Costa; Alcântara, Diego Á.; Cunha, Luiz Raimundo; Lima, Patrícia Lima de; Vasconcellos, Marne Carvalho de; Montenegro, Raquel Carvalho; Soares, Bruno Moreira; Amorim, M.; Burbano, Rommel Rodríguez

doi:10.1007/978-1-4939-1068-7_7

Cited by 4 publications

(4 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All reads showed to be significant (P<0.001) for all treatment in compare with control. These results reflects that Fasciola hepatica acetone powder have genotoxicity effect leads to these chromosomal aberration like many organic substances derived from other organism like plants with other methods of extraction [25] or for Amoeba parasite [26] but not by acetone powder method. These effects may explain in some manner how carcinogenic property of chronic infection with Fascioliasis above liver of their host which may be related to release of toxic parasite substances through various growing phases of it in parasitized location as for liver in case of liver fluke here.…”

Section: Resultsmentioning

confidence: 91%

Chromosomal Aberration of Fasciola Hepatica Acetone Powder: In Vitro Study on Human Lymphocytes

Al- Bayati Saad Mohi Haider,

Ali Mohammed Rafeik

2021

Indian Journal of Forensic Medicine & Toxicology

View full text Add to dashboard Cite

Fasciola hepatica metazoan trematodes parasite that is living in the liver of their host (sheep, goat and human). It usually cause pathological changes in the parasitized organs. Acetone powder technique is used to prepare partially purified organic material of adult worms. Various concentrations of Fasciola hepatica acetone extract (5 mg/ml, 10 mg /ml , 15mg/ml , 20 mg/ml and 40 mg /ml) are used on human lymphocyte culture for studying some chromosomal aberration. Chromosomal aberrations includes Isogap, Breaks and Dicentrics for chromosomes; Gaps and deletion for chromatids; and mitotic index (MI). This In-vitro study revealed that acetone powder extract of Fasciola hepatica parasites causing various genotoxicity effects in the human lymphocyte cells. These effects are ascending in their increasing in significance differences (P< 0.001) for all treatment against control as concentration of acetone extract is increased.

show abstract

Section: Resultsmentioning

confidence: 91%

Chromosomal Aberration of Fasciola Hepatica Acetone Powder: In Vitro Study on Human Lymphocytes

Al- Bayati Saad Mohi Haider,

Ali Mohammed Rafeik

2021

Indian Journal of Forensic Medicine & Toxicology

View full text Add to dashboard Cite

show abstract

“…In occupational and environmental assessments, the chromosomal aberration test has been utilized for decades as a biomarker of the early impacts of genotoxic agents (Guimaraes et al, 2014; Norppa et al, 2006). Our experimental conclusions showed that these two phthalates caused more clastogenic effects in CHO cells than the vehicle control, as measured by the proportion of aberrant metaphases and the overall number of aberrations.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Clastogenic, aneugenic, and tubulin polymerization properties of di-(2-ethylhexyl) phthalate and dibutyl phthalate

Javaji,

Mamilla,

Deshpande

et al. 2023

Toxicol Ind Health

View full text Add to dashboard Cite

Phthalate compounds were found to disrupt the endocrine system and alter transcriptomes during human embryonic development. In our previous work, we have isolated and reported two such phthalates di-(2-ethylhexyl) phthalate (DEHP) and dibutyl phthalate (DBP) from Brevibacterium mcbrellneri bacteria and evaluated their bioactive properties. Naturally derived phthalates might be less toxic compared with synthesized molecules. We have investigated biologically isolated phthalates to understand the possible genotoxic effects in mice and further investigated in silico binding and polymerization of β-tubulin. Three sub-lethal concentrations of DEHP (150 μM, 175 μM, and 200 μM) and DBP (10 μM, 15 μM, and 30 μM) were studied. The results showed that the phthalates were found to be highly genotoxic in nature. However, the pattern of genotoxic effects was not found to be dose-dependent in the induction of chromosome aberrations (CA), micronuclei (MN), and changes in the mitotic index (MI) in cells. In silico studies of phthalates on polymerization of β-tubulin suggested that both DBP and DEHP were able to interact with the hydrogen bonds and make strong van der Waals interactions with β-tubulin thereby possibly causing destabilization of microtubule network. Our study suggests that these phthalates might be playing an important role in normal cell division thereby showing highly genotoxic effects.

show abstract

“…Culture initiation was done with 80% of RPMI 1640 media, 20% of FBS, 400µL of Phytohemagglutinin (PHA) and 1ml of peripheral blood was added to the culture flask along with varied concentration of purified sample (50, 100, 200 and 400 µg/ ml) and Positive control ethyl methanesulphonate (EMS) and a media, serum and blood with the PHA was maintained as negative control. 10 The culture flasks were incubated at 37°C for 48 hr with 5% CO 2 . Colchicine (0.2 ml) was added to block the cells in metaphase.…”

Section: Chromosomal Aberration Assaymentioning

confidence: 99%

In vitro Toxicity Evaluation of Sterol Isolate from Echinoderm Stellaster equestris against Human Peripheral Blood

Sumitha

Banu

Vijayalakshmi

et al. 2021

JPHI

View full text Add to dashboard Cite

Background: Ocean acts as a wealthy resource of biological diversity producing prospective novel metabolites from the organisms present in the environment. Novel bioactive compounds from Echinoderms mainly from sea stars have been extensively studied procuring rich diverse compounds which exhibits in vitro cytotoxicity comparable to or better than those of the potent anticancer drug. These sea stars which reside in the benthic region serves as their habitat and they are therefore predators and persistently being attacked by various organisms subsequently they acclimatize the survival approach to defend themselves from the external pathogens. Since these metabolites are obscured as a metabolic product during their endurance an initial evaluation of these compounds is necessary to assess human risk. Objectives: The present study focuses in vitro toxicity evaluation of isolated sterol like isolate from echinoderm sea star Stellaster equestris by incorporating hemolytic, chromosomal aberration assay and the cell viability tryphan blue exclusion assay against human peripheral blood. Methods: The in vitro toxicity evaluation was studied against the human peripheral blood collected from the healthy donors with the defined concentration of the sterol like compound from the sea star Stellaster equestris. The hemolytic, cell viability trypan blue exclusion method and chromosomal aberration assay were performed to check the hemolytic, mutagenic and genotoxic effect against the lymphocytes and the red blood cells. Results and Discussion: The result suggested that the hemolytic assay and the cell viability assay even in a dose dependent manner were non-hemolytic and percentage of the viability was not affected due to the exposure of the compound. The absence of genotoxicity was evident for the chromosomal aberration assay indicated that the isolated compound from the sea star Stellaster equestris might be considered as effective novel compound.

show abstract

Chromosomal Aberration Test Utilities In Vitro and In Vivo

Cited by 4 publications

References 53 publications

Chromosomal Aberration of Fasciola Hepatica Acetone Powder: In Vitro Study on Human Lymphocytes

Chromosomal Aberration of Fasciola Hepatica Acetone Powder: In Vitro Study on Human Lymphocytes

Clastogenic, aneugenic, and tubulin polymerization properties of di-(2-ethylhexyl) phthalate and dibutyl phthalate

In vitro Toxicity Evaluation of Sterol Isolate from Echinoderm Stellaster equestris against Human Peripheral Blood

Contact Info

Product

Resources

About