Chromosomal aberrations in mouse lymphocytes exposed in vivo and in vitro to aliphatic epoxides

Das, L.M.; Das, Salil K.; Chu, Ernest H. Y.; Sinsheimer, Joseph E.

doi:10.1016/0165-1218(93)90114-s

Cited by 4 publications

(1 citation statement)

References 23 publications

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“…In this context, aromatic polycyclic hydrocarbons have been studied extensively. This is illustrated by a literature search covering this topic over the period from 1980 to 1995, for which 2391 references were found. − This is in contrast to the results obtained for simple unsaturated epoxides, with only 30 references. − …”

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confidence: 96%

Analysis of DNA Adducts in DNA Hydrolysates by Capillary Zone Electrophoresis and Capillary Zone Electrophoresis−Electrospray Mass Spectrometry

Deforce¹,

Ryniers²,

Eeckhout³

et al. 1996

Anal. Chem.

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The in vitro adduct formation with phenyl glycidyl ethers (PGEs) was studied on 2'-deoxynucleotides and DNA. The modified DNA was hydrolyzed enzymatically, and the mixtures consisting of unmodified 2'-deoxynucleotide adducts were analyzed by capillary zone electrophoresis (CZE), CZE-electrospray mass spectrometry (CZE/ES-MS) and CZE-electrospray tandem mass spectrometry (CZE/ES-MS/MS) using sample stacking. For the CZE analyses, a homemade system was developed in order to enhance the reproducibility of the retention times. This modification enabled the total comparison of the electropherograms obtained for the analysis of 2'deoxynucleotides mixtures with the electropherograms obtained for the DNA hydrolysates both treated with PGEs. The assignment of adducted and nonadducted 2'-deoxynucleotide peaks was unambiguous. Analysis of the CZE/ES-MS data gave the necessary structural information and revealed the presence of mono- and dialkylated 2'-deoxynucleotides. Interpretation of the CZE/ES-MS/MS data of the monoalkylated products allowed differentiation between purine or pyrimidine alkylation and alkylation of the 5-phosphate moiety. Recording of full-scan mass spectra during CZE/ES-MS/MS analysis of 2'-deoxynucleotide reaction mixtures and DNA hydrolysates was possible, using the described CZE sample stacking technique.

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confidence: 96%

Analysis of DNA Adducts in DNA Hydrolysates by Capillary Zone Electrophoresis and Capillary Zone Electrophoresis−Electrospray Mass Spectrometry

Deforce¹,

Ryniers²,

Eeckhout³

et al. 1996

Anal. Chem.

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p ‐Nitrophenyl Glycidyl Ether 5255‐75‐4

2004

Sax's Dangerous Properties of Industrial Materials

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Chromosomal aberrations in mouse lymphocytes exposed in vitro and in vivo to benzidine and 5 related aromatic amines

Das

Hooberman

et al. 1994

Mutation Research/Genetic Toxicology

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Mouse lymphocytes were exposed in vitro for 2 h or in vivo for 24 h to benzidine and related aromatic amines to test for chromosome aberrations (CA) and mitotic indices. Uninduced mouse S9 was used to activate the amines for the in vitro tests to be consistent with the in vivo tests. Contrary to a previous report, no difference could be established in the genotoxicity of benzidine following activation with uninduced S9 compared to induced S9. There were concentration related increases in CA for benzidine and all the amines in vitro except for 4,4'-diaminostilbene which exhibited the greatest cellular toxicity towards cultured lymphocytes. Benzidine and its derivatives showed significant increases in CA in vivo compared to its negative control. The CA values for 4-aminostilbene were significantly higher than the other amines in both in vivo and in vitro studies. These genotoxicity results for 4-aminostilbene are consistent with our previous report of the pronounced CA effects in murine bone-marrow cells but would not be predicted from Salmonella mutagenicity tests.

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Chromosomal aberrations in mouse lymphocytes exposed in vivo and in vitro to aliphatic epoxides

Cited by 4 publications

References 23 publications

Analysis of DNA Adducts in DNA Hydrolysates by Capillary Zone Electrophoresis and Capillary Zone Electrophoresis−Electrospray Mass Spectrometry

Analysis of DNA Adducts in DNA Hydrolysates by Capillary Zone Electrophoresis and Capillary Zone Electrophoresis−Electrospray Mass Spectrometry

p ‐Nitrophenyl Glycidyl Ether 5255‐75‐4

Chromosomal aberrations in mouse lymphocytes exposed in vitro and in vivo to benzidine and 5 related aromatic amines

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