Chromosomal Abnormalities in Human Lymphocytes after Computed Tomography Scan Procedure

Shi, Lin; Fujioka, Kurumi; Sakurai-Ozato, Nami; Fukumoto, Wataru; Satoh, Kenichi; Sun, Jiying; Awazu, Akinori; Tanaka, Kimio; Ishida, Mari; Ishida, Takafumi; Nakano, Yukiko; Kikuchi, Yoshikazu; Hayes, C.; Aikata, Hiroshi; Chayama, Kazuaki; Ito, Takashi; Awai, Kazuo; Tashiro, Satoshi

doi:10.1667/rr14976.1

Cited by 24 publications

(17 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even though the mean values of all patients as a group for all three endpoints tested show increased yields relative to baseline following medical exposure, our results demonstrate that only the γ-H2AX biomarker enables detection of statistically The present observations agree with cytogenetic studies carried out in patients exposed to low IR doses. Particularly, according some studies the numbers of dicentric and ring chromosomes were significantly increased after a CT scan in patients (13,19,(27)(28)(29). In our study, after the cardiac interventional procedures, which have resulted in a mean dose of about 14 mSv a total of 48.000 cells have been analyzed.…”

Section: Discussionmentioning

confidence: 67%

The Use of Genotoxicity Endpoints as Biomarkers of Low Dose Radiation Exposure in Interventional Cardiology

Habibi

Karyofyllis

Nikolakopoulou

et al. 2021

Front. Public Health

View full text Add to dashboard Cite

The effect of the reportedly low ionizing radiation doses, such as those very often delivered to patients in interventional cardiology, remains ambiguous. As interventional cardiac procedures may have a significant impact on total collective effective dose, there are radiation protection concerns for patients and physicians regarding potential late health effects. Given that very low doses (<100 mSv) are expected to be delivered during these procedures, the purpose of this study was to assess the potency and suitability of current genotoxicity biomarkers to detect and quantitate biological effects essential for risk estimation in interventional cardiology. Specifically, the biomarkers γ-H2AX foci, dicentric chromosomes, and micronuclei, which underpin radiation-induced DNA damage, were studied in blood lymphocytes of 25 adult patients before and after interventional cardiac procedures. Even though the mean values of all patients as a group for all three endpoints tested show increased yields relative to baseline following medical exposure, our results demonstrate that only the γ-H2AX biomarker enables detection of statistically significant differences at the individual level (p < 0.001) for almost all patients (91%). Furthermore, 24 h after exposure, residual γ-H2AX foci were still detectable in irradiated lymphocytes. Their decline was found to vary significantly among the individuals and the repair kinetics of γ-H2AX foci was found to range from 25 to 95.6% of their maximum values obtained.

show abstract

Section: Discussionmentioning

confidence: 67%

The Use of Genotoxicity Endpoints as Biomarkers of Low Dose Radiation Exposure in Interventional Cardiology

Habibi

Karyofyllis

Nikolakopoulou

et al. 2021

Front. Public Health

View full text Add to dashboard Cite

show abstract

“…Although the characteristics of participants who underwent LD CT and/or SD CT were not significantly different, there was a slight difference in the number of CAs before CT. Consequently, on the basis of observations reported by Shi et al (28), CT the CA number was 7.8 (IQR, 4.4211.0) and 9.2 (IQR, 6.5213.9), respectively (P = .009).…”

Section: Semiautomatic Counting Of Chromosome Aberrationsmentioning

confidence: 67%

“…g-H2AX is a marker of DNA double-strand break; CAs reflect both the damage from radiation exposure and the accuracy of DNA repair. Individual variations in the response to DNA damage (18,28) may explain the absence of a correlation between the number of g-H2AX foci and CAs after CT and the dose-length product and the degree of DNA damage.…”

Section: Semiautomatic Counting Of Chromosome Aberrationsmentioning

confidence: 99%

Biological Effects of Low-Dose Chest CT on Chromosomal DNA

Sakane¹,

Ishida²,

Shi³

et al. 2020

Radiology

Self Cite

View full text Add to dashboard Cite

L ung cancer is the leading cause of cancer deaths (1). Its early detection and treatment are important to reduce the mortality rate. According to the National Lung Screening Trial, annual lung cancer screening by using low-dose (LD) chest CT reduced the number of deaths in patients at high risk (2). LD CT screening is not without risk and although the associated radiation dose is about one-fourth that of standard-dose (SD) CT (3), on the basis of the linear nonthreshold theory, the cancer risk from radiation exposure at LD CT cannot be ignored. Annual screening has been suggested to increase the risk for lung cancer (4,5), although the radiation-induced cancer risk from exposure to less than 100 mSv remains unclear (6,7).g-H2AX analysis to evaluate the biologic effects of lowdose radiation including CT revealed that exposure even at the diagnostic level induces DNA damage (8-14). Dicentric and ring chromosome analyses are the reference standard in biodosimetry. Immunofluorescence analysis of nuclear foci formed by the phosphorylated histone H2AX (g-H2AX), a technically simple and highly sensitive method, yields a marker of DNA double-strand breaks (15). The effects of LD CT on DNA damage and chromosome aberrations (CAs) in adults remain to be clarified before the implementation of

show abstract

“…Since this reduction at 24 hours in the γ-H2AX score was not observed in the very-low-dose group, we could not clearly state that continuous low-dose radiation decreased the DSB, but it was suggested that DSBs were efficiently repaired after 6 hours in the cells cultured on the radiation-emitting sheets. When DSB and chromosome damage are evaluated immediately or shortly after low-dose (<100 mGy) radiation, increases in the indices may be observed, 22,23 but the damage may be efficiently repaired thereafter or damaged cells may be eliminated by apoptosis and replaced with new cells by the effect of low-dose radiation. Overall, low-dose radiation may enhance the DNA repair capacity and contribute to repair of DSB following subsequent exposure to a high dose of radiation.…”

Section: Discussionmentioning

confidence: 99%

Changes in the Proliferation Rate, Clonogenicity, and Radiosensitivity of Cultured Cells During and After Continuous Low-Dose-Rate Irradiation

et al. 2019

View full text Add to dashboard Cite

We investigated the effects of continuous low-dose radiation on proliferation, clonogenicity, radiosensitivity, and repair of DNA double-strand breaks (DSBs) in human salivary gland (HSG) tumor cells. Human salivary gland cells were cultured on acrylic boards above very-low-dose (4.3 μSv/h) or low-dose (27 μSv/h) radiation-emitting sheets or without sheets. Total cell numbers and plating efficiencies were compared among the 3 groups every 1 or 2 weeks until 6 weeks after starting culture. At 2, 4, and 6 weeks, surviving fractions of HSG cells after irradiation at 2 to 8 Gy cultured on the very-low-dose or low-dose sheets were compared to those of the control. At 4 weeks, HSG cells irradiated at 2 Gy were assessed for phosphorylated histone (γH2AX) foci formation, and DSBs were evaluated. No significant differences were observed in total cell number or plating efficiencies with or without low-dose-emitting sheets. The surviving fractions after irradiation of the very-low-dose group at 2 to 6 weeks and those of the low-dose group at 2 to 4 weeks were higher than those of the control ( P < .01). Thus, a radioadaptive response was clearly demonstrated. From the γH2AX foci quantification, the adaptive responses were considered to be associated with the efficient repair of DSB, especially slow repair, in this cell line.

show abstract

Chromosomal Abnormalities in Human Lymphocytes after Computed Tomography Scan Procedure

Cited by 24 publications

References 48 publications

The Use of Genotoxicity Endpoints as Biomarkers of Low Dose Radiation Exposure in Interventional Cardiology

The Use of Genotoxicity Endpoints as Biomarkers of Low Dose Radiation Exposure in Interventional Cardiology

Biological Effects of Low-Dose Chest CT on Chromosomal DNA

Changes in the Proliferation Rate, Clonogenicity, and Radiosensitivity of Cultured Cells During and After Continuous Low-Dose-Rate Irradiation

Contact Info

Product

Resources

About