Chromosomal and flavonoid chemical confirmation of intergeneric hybridization between <i>Tolmiea</i> and <i>Tellima</i> (Saxifragaceae)

Soltis, Douglas E.; Bohm, Bruce A.

doi:10.1139/b85-182

Cited by 11 publications

(6 citation statements)

References 10 publications

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“…Clearly, several clades found in the strict consensus of cpDNA and ITS sequence trees represent strongly supported relationships: (1) Tolmiea 2x and 4x; (2) M. ovalis and M. breweri; and (3) L. parviflorum and L. affine. These sister groups all are in agreement with long-standing views based on morphology and in some cases chemical data (e.g., Soltis and Bohm 1985;Rosendahl 1914;Small and Rydberg 1905).…”

Section: Systematic Implications For the Heuchera Groupsupporting

confidence: 88%

“…Second, the occurrence of chloroplast capture agrees with the basic biology of the Heuchera group. Genera such as Heuchera are well known for frequent interspecific hybridization (Rosendahl et al 1936), and intergeneric hybrids have been reported between Tolmiea and Tellima (Soltis and Bohm 1985), Mitella and Conimitella (Soltis and Soltis 1986), Heuchera and Mitella, and Heuchera and Tiarella (reviewed in Spongberg 1972). Although such intergeneric hybrids are rare, they nonetheless illustrate the potential for hybridization and chloroplast capture to occur on a broad scale throughout the group.…”

Section: Discussionmentioning

confidence: 99%

“…The largest two genera, Mitella and Heuchera, consist of approximately 20 and 50 species, respectively. Six of these genera are known to hybridize (Spongberg 1972;Soltis and Bohm 1985;Soltis and Soltis 1986), and numerous interspecific hybrids have been reported within Heuchera (Rosendahl et al 1936).…”

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confidence: 99%

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DISCORDANCE BETWEEN NUCLEAR AND CHLOROPLAST PHYLOGENIES IN THEHEUCHERAGROUP (SAXIFRAGACEAE)

1995

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Various factors, including taxon density, sampling error, convergence, and heterogeneity of evolutionary rates, can potentially lead to incongruence between phylogenetic trees based on different genomes. Particularly at the generic level and below, chloroplast capture resulting from hybridization may distort organismal relationships in phylogenetic analyses based on the chloroplast genome, or genes included therein. However, the extent of such discord between chloroplast DNA (cpDNA) trees and those trees based on nuclear genes has rarely been assessed. We therefore used sequences of the internal transcribed spacer regions (ITS-1 and ITS-2) of nuclear ribosomal DNA (rDNA) to reconstruct phylogenetic relationships among members of the Heuchera group of genera (Saxifragaceae). The Heuchera group presents an important model for the analysis of chloroplast capture and its impact on phylogenetic reconstruction because hybridization is well documented within genera (e.g., Heuchera), and intergeneric hybrids involving six of the nine genera have been reported. An earlier study provided a well-resolved phylogenetic hypothesis for the Heuchera group based on cpDNA restriction-site variation. However, trees based on ITS sequences are discordant with the cpDNA-based tree. Evidence from both morphology and nuclear-encoded allozymes is consistent with the ITS trees, rather than the cpDNA tree, and several points of phylogenetic discord can clearly be attributed to chloroplast capture. Comparison of the organellar and ITS trees also raises the strong likelihood that ancient events of chloroplast capture occurred between lineages during the early diversification of the Heuchera group. Thus, despite the many advantages and widespread use of cpDNA data in phylogeny reconstruction, comparison of relationships based on cpDNA and ITS sequences for the Heuchera group underscores the need for caution in the use of organellar variation for retrieving phylogeny at lower taxonomic levels, particularly in groups noted for hybridization.

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Section: Systematic Implications For the Heuchera Groupsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

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DISCORDANCE BETWEEN NUCLEAR AND CHLOROPLAST PHYLOGENIES IN THEHEUCHERAGROUP (SAXIFRAGACEAE)

1995

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“…and the remaining four monotypic genera (Bensoniella, Conimitella, Elmera, and Tellima) are solely distributed in western North America. Six of these genera are known to hybridize (Soltis and Bohm, 1985;Soltis and Soltis, 1986;Soltis et al, 1991), with intergeneric hybrids being reported between Heuchera and Tiarella (Spongberg, 1972), Tellima and Tolmiea (Soltis and Bohm, 1985), and Mitella and Conimitella (Soltis and Soltis, 1986). Numerous interspecific hybrids have been reported within Heuchera (Rosendahl et al, 1936).…”

Section: Introductionmentioning

confidence: 99%

Plastome Evolution in Saxifragaceae and Multiple Plastid Capture Events Involving Heuchera and Tiarella

Liu

Folk

et al. 2020

Front. Plant Sci.

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Saxifragaceae, a family of over 600 species and approximately 30 genera of herbaceous perennials, is well-known for intergeneric hybridization. Of the main lineages in this family, the Heuchera group represents a valuable model for the analysis of plastid capture and its impact on phylogeny reconstruction. In this study, we investigated plastome evolution across the family, reconstructed the phylogeny of the Heuchera group and examined putative plastid capture between Heuchera and Tiarella. Seven species (11 individuals) representing Tiarella, as well as Mitella and Heuchera, were selected for genome skimming. We assembled the plastomes, and then compared these to six others published for Saxifragaceae; the plastomes were found to be highly similar in overall size, structure, gene order and content. Moreover, ycf15 was lost due to pseudogenization and rpl2 lost its only intron for all the analyzed plastomes. Comparative plastome analysis revealed that size variations of the plastomes are purely ascribed to the length differences of LSC, SSC, and IRs regions. Using nuclear ITS + ETS and the complete plastome, we fully resolved the species relationships of Tiarella, finding that the genus is monophyletic and the Asian species is most closely related to the western North American species. However, the position of the Heuchera species was highly incongruent between nuclear and plastid data. Comparisons of nuclear and plastid phylogenies revealed that multiple plastid capture events have occurred between Heuchera and Tiarella, through putative ancient hybridization. Moreover, we developed numerous molecular markers for Tiarella (e.g., plastid hotspot and polymorphic nuclear SSRs), which will be useful for future studies on the population genetics and phylogeography of this disjunct genus.

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“…Gray, Mitella L., and Heuchera L.). This included intergeneric hybridization between Heuchera and Tiarella , Tellima and Tolmiea , Mitella and Conimitella , and interspecific hybridization in Heuchera (e.g., Rosendahl et al, 1936; Spongberg, 1972; Soltis & Bohm, 1985; Soltis & Soltis, 1986). No hybridization events have been reported in Mukdenia species, but we cannot rule it out here.…”

Section: Discussionmentioning

confidence: 99%

Systematics of Mukdenia and Oresitrophe (Saxifragaceae): Insights from genome skimming data

Liu

Deng

Chen

et al. 2022

J of Sytematics Evolution

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Oresitrophe and Mukdenia (Saxifragaceae) are epilithic sister genera used in traditional Chinese medicine. The taxonomy of Mukdenia, especially of M. acanthifolia, has been controversial. To address this, we produced plastid and mitochondrial data using genome skimming for Mukdenia acanthifolia and Mukdenia rossii, including three individuals of each species. We assembled complete plastomes, mitochondrial CDS and nuclear ribosomal ETS/ITS sequences using these data. Comparative analysis shows that the plastomes of Mukdenia and Oresitrophe are relatively conservative in terms of genome size, structure, gene content, RNA editing sites and codon usage. Five plastid regions that represent hotspots of change (trnH‐psbA, psbC‐trnS, trnM‐atpE, petA‐psbJ and ccsA‐ndhD) are identified within Mukdenia, and six regions (trnH‐psbA, petN‐psbM, trnM‐atpE, rps16‐trnQ, ycf1 and ndhF) contain a higher number of species‐specific parsimony‐informative sites that may serve as potential DNA barcodes for species identification. To infer phylogenetic relationships between Mukdenia and Oresitrophe, we combined our data with published data based on three different datasets. The monophyly of each species (Oresitrophe rupifraga, M. acanthifolia and M. rossii) and the inferred topology ((M. rossii, M. acanthifolia), O. rupifraga) are well supported in trees reconstructed using the complete plastome sequences, but M. acanthifolia and M. rossii did not form a separate clade in the trees based on ETS + ITS data, while the mitochondrial CDS trees are not well‐resolved. We found low recovery of genes in the Angiosperms353 target enrichment panel from our unenriched genome skimming data. Hybridization or incomplete lineage sorting may be the cause of discordance between trees reconstructed from organellar and nuclear data. Considering its morphological distinctiveness and our molecular phylogenetic results, we strongly recommend that M. acanthifolia be treated as a distinct species.

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Chromosomal and flavonoid chemical confirmation of intergeneric hybridization between Tolmiea and Tellima (Saxifragaceae)

Cited by 11 publications

References 10 publications

DISCORDANCE BETWEEN NUCLEAR AND CHLOROPLAST PHYLOGENIES IN THEHEUCHERAGROUP (SAXIFRAGACEAE)

DISCORDANCE BETWEEN NUCLEAR AND CHLOROPLAST PHYLOGENIES IN THEHEUCHERAGROUP (SAXIFRAGACEAE)

Plastome Evolution in Saxifragaceae and Multiple Plastid Capture Events Involving Heuchera and Tiarella

Systematics of Mukdenia and Oresitrophe (Saxifragaceae): Insights from genome skimming data

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