Chromosomal constitution of mouse blastocysts derived from oocytes inseminated by multiple sperm insertion into the perivitelline space

Macas, Ervin; Rosselli, Marinella; Imthurn, Bruno; Keller, Paul J.

doi:10.1007/bf01212935

Cited by 10 publications

(6 citation statements)

References 34 publications

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“…Postzygotic diploidization of triploids was also described by Grossmann et al who reported a significant proportion (36%) of 3PN ICSIderived zygotes that were actually diploid with respect to chromosome number [32]. These results are also consistent with Macas et al [23] who reported a 32% incidence of postzygotic diploidization of triploids. Postzygotic diploidization of triploids may arise from the abnormal distribution of the maternal chromosomes into two separate sets after extrusion of the first PB [32].…”

Section: Discussionsupporting

confidence: 92%

“…Additionally, the early cleavage divisions in human and mouse embryos have been shown to be error-prone and could contribute to the formation of mosaic embryos [19]. Studies also suggest that 2n/4n mosaicism is a normal feature of the later-stage embryos and that there is a nonrandom allocation of the tetraploid cells within the embryo [17,[20][21][22][23]. Ruangvutilert et al [17] described the presence of tetraploid cells in a significant proportion of arrested cleavage-stage human embryos (6/20) and at an even higher proportion of human blastocyst stage embryos (14/19).…”

Section: Discussionmentioning

confidence: 96%

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Evaluation of the developmental competence and chromosomal compliment of mouse oocytes derived from in-vitro growth and maturation of preantral follicles

Liu

Aoki

Carrell

2008

J Assist Reprod Genet

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 96%

Evaluation of the developmental competence and chromosomal compliment of mouse oocytes derived from in-vitro growth and maturation of preantral follicles

Liu

Aoki

Carrell

2008

J Assist Reprod Genet

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“…Haploidy has been observed in the embryos of domestic animals and humans with frequencies of 1% to 22%. The highest percentage of haploidy reported until now -22% -was observed by Macas et al (1993) when analysing mouse blastocysts from oocytes inseminated by multiple injection in the perivitelline space. In 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 Cytogenetic analysis of caprine embryos produced in vitro 197 pigs, haploid embryos accounted for 12.2% of embryos (Underhill et al, 1991), in sheep, 2.7% (Murray et al, 1985) and in human embryos, 2.6% (Jamiesson et al, 1994) and 11.8% (Pellestor et al, 1994b).…”

Section: Discussionmentioning

confidence: 95%

Cytogenetic analysis of caprine 2- to 4-cell embryos produced in vitro

Villamediana

Vidal

Paramio

2001

Zygote

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Prepubertal goat in vitro matured/in vitro fertilised oocytes produce only a small percentage of blastocysts. The present study examines the incidence of chromosomal anomalies in 2- to 4-cell embryos in vitro produced (IVP) from prepubertal oocytes fertilised with the semen of two males. Cumulus-oocyte complexes were obtained by slicing ovaries from slaughtered prepubertal goats. Oocytes were matured in TCM199 supplemented with 20% heat inactivated Donor Bovine Serum (DBS), 10 μg/ml FSH + 10 μg/ml LH + 1 μg/ml 17β-oestradiol for 27 h at 38.5 °C in 5% CO2 in air. IVM oocytes were inseminated with the sperm from two males prepared using the swim-up and heparin-capacitation procedures. At 24 h post-insemination (hpi) the oocytes were transferred to 100 μl drops of SOF medium for a further 24 h. At 17 hpi a sample of oocytes was stained with lacmoid to evaluate the nuclear stage after fertilisation. The cleavage rate was determined at 24, 36 and 48 hpi and chromosome slides were prepared according to the gradual-fixation technique and stained with Leishman. A total of 1070 2- to 4-cell embryos from prepubertal goat oocytes were studied, but it was only possible to analyse 241 cytogenetically. Of these, 40% exhibited a normal diploid chromosome complement, 59% were haploid and 1% were triploid. There were significant differences between the two males in sperm oocyte penetration and oocyte cleavage but no differences were found in chromosomal anomalies. In conclusion, the low number of embryos karyotyped and the high number of haploid embryos found in this study suggested a high incidence of abnormal fertilised embryos and deficient cytoplasmic maturation of the oocyte which inhibits sperm head decondensation.

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“…See text for details. polar body and the two pronuclei was checked for every zygote to ensure normal fertilization and to exclude parthenogenetic activation of oocytes (Macas et al, 1993). The zygotes were then cultured (in Ham F 10 medium with 10 per cent human foetal cord serum at 37°C and 5 per cent CO2 in air) until they reached the blastula stage.…”

Section: Methodsmentioning

confidence: 99%

Non-random fertilization in mice correlates with the MHC and something else

et al. 1996

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One evolutionary explanation for the success of sexual reproduction assumes that sex is an advantage in the coevolutionary arms race between pathogens and hosts. Accordingly, an important criterion in mate choice and maternal selection thereafter could be the allelic specificity at polymorphic loci involved in parasite-host interactions, e.g. the MHC (major histocompatibility complex). The MHC has been found to influence mate choice and selective abortions in mice and humans. However, it could also influence the fertilization process itself, i.e. (i) the oocyte's choice for the fertilizing sperm, and (ii) the outcome of the second meiotic division after the sperm has entered the egg. We tested both hypotheses in an in vitro fertilization experiment with two inbred mouse strains congenic for their MHC. The genotypes of the resulting blastocysts were determined by polymerase chain reaction. We found nonrandom MHC combinations in the blastocysts which may result from both possible choice mechanisms. The outcome changed significantly over time, indicating that a choice for MHC combinations during fertilization may be influenced by one or several external factors.

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Chromosomal constitution of mouse blastocysts derived from oocytes inseminated by multiple sperm insertion into the perivitelline space

Abstract: Mosaicism and parthenogenesis appear to be increased significantly following microinjection of multiple spermatozoa under the zona pellucida of mouse oocytes, and there was no evidence of aneuploidy.

Cited by 10 publications

References 34 publications

Evaluation of the developmental competence and chromosomal compliment of mouse oocytes derived from in-vitro growth and maturation of preantral follicles

Evaluation of the developmental competence and chromosomal compliment of mouse oocytes derived from in-vitro growth and maturation of preantral follicles

Cytogenetic analysis of caprine 2- to 4-cell embryos produced in vitro

Non-random fertilization in mice correlates with the MHC and something else

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