Chromosomal distribution of Drosophila HMG-like proteins

Abstract

Fractionation of nucleosomes in preparative agarose gels Method 1 Method 2 Comments on the two methods Analytical agarose gel electrophoresis Polyacrylamide gel electrophoresis Triton/acid/urea first dimension tube gels 30 Acid-soluble nuclear protein samples 31 Agarose fractionated nucleosomal samples 31 Triton/acid/urea slab gels SDS slab gels Staining of proteins Coomassie Brilliant Blue R staining SiIver staining 32 DNA electrophoresis Photography Determination of Materials 34 Acrylamide Other reagents RESULTS I. ELECTROPHORESIS OF ACID-SOLUBLE NUCLEAR PROTEINS RESULTS II. PURIFICATION OF PUTATIVE HMG PROTEINS RESULTS III. IMMUNOFLUORESCENCE STUDIES OF A13 RESULTS IV.