Chromosomal distribution of pTa-535, pTa-86, pTa-713, 35S rDNA repetitive sequences in interspecific hexaploid hybrids of common wheat (Triticum aestivum L.) and spelt (Triticum spelta L.)

Abstract

Fluorescent in situ hybridization (FISH) relies on fluorescent-labeled probes to detect specific DNA sequences in the genome, and it is widely used in cytogenetic analyses. The aim of this study was to determine the karyotype of T. aestivum and T. spelta hybrids and their parental components (three common wheat cultivars and five spelt breeding lines), to identify chromosomal aberrations in the evaluated wheat lines, and to analyze the distribution of polymorphisms of repetitive sequences in the examined hybrids. The FISH procedure was carried out with four DNA clones, pTa-86, pTa-535, pTa-713 and 35S rDNA used as probes. The observed polymorphisms between the investigated lines of common wheat, spelt and their hybrids was relatively low. However, differences were observed in the distribution of repetitive sequences on chromosomes 4A, 6A, 1B and 6B in selected hybrid genomes. The polymorphisms observed in common wheat and spelt hybrids carry valuable information for wheat breeders. The results of our study are also a valuable source of knowledge about genome organization and diversification in common wheat, spelt and their hybrids. The relevant information is essential for common wheat breeders, and it can contribute to breeding programs aimed at biodiversity preservation.

Development and Cytomolecular Identification of Monosomic Alien Addition and Substitution Lines of Triticale (×Triticosecale Wittmack) With 2Sk Chromosome Conferring Leaf Rust Resistance Derived From Aegilops kotschyi Boiss

“…Chromosomes were identified using fluorescence in situ hybridization (FISH) with the repetitive sequences from pTa-86, pTa-535, pTa-103 (centromere specific), and pTa-k374 (homologous to ribosomal DNA sequence 28S) clones described by Komuro et al (2013) . Clones were amplified from genomic DNA of “Chinese Spring” wheat ( Kwiatek et al, 2016b ; Goriewa-Duba et al, 2018 ) and labeled using Atto-488NT, Atto-550NT, and Atto-647NT nick translation kits (Jena Bioscience, Germany). FISH/genomic in situ hybridization (GISH) experiments were performed according to Kwiatek et al (2017b) .…”

Improvement of the process of hydrothermal treatment and peeling of spelt wheat grain during cereal production

“…Spelt wheat grain is of great value for selection and improvement of the quality of traditional wheat varieties [12]. Obtained hybrids enable the substantial expansion of the range of food products and improvement of their quality [13].…”

Cytological markers used for identification and transfer of Aegilops spp. chromatin carrying valuable genes into cultivated forms of Triticum

“…Tang et al (2014) designed oligonucleotides to replace the repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for Aegilops-Triticum chromosome identification. Kwiatek et al (2016b) and Goriewa-Duba et al (2018) developed specific primers to amplify some of the repetitive sequences reported by Komuro et al (2013) from wheat genomic DNA. This approach reduces the time and the costs of BAC library maintenance.…”