Chromosomal DNA fingerprinting--a new method of species and strain identification applicable to microbial pathogens

Owen, Robert J.

doi:10.1099/00222615-30-2-89

Cited by 127 publications

(77 citation statements)

References 27 publications

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“…rDNA fingerprinting is used increasingly for classification (7,8,12). Our results provided further evidence of the high level of homology between L .…”

Section: Resultssupporting

confidence: 72%

Assignment of Listeria grayi and Listeria murrayi to a Single Species, Listeria grayi, with a Revised Description of Listeria grayi

Rocourt

Boerlin

Grimont

et al. 1992

International Journal of Systematic Bacteriology

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The genomic relatedness between Listeria gruyi and Listenu murruyi was reevaluated by using DNA-DNA hybridization, multilocus enzyme electrophoresis, and rRNA restriction fragment length polymorphism techniques. The high levels of similarity observed between the strains of these two species confirmed the data published since 1973 and indicated that they should be considered members of a single species. On grounds of priority, the species should be named L. gruyi. and subsequently proposed that they should be placed in a single species (22). However, this proposal was not put into effect on the Approved Lists of Bacterial Names (20), nor was it validated by a subsequent publication, possibly because Stuart and Welshimer (22) proposed exclusion of these two species from the genus Listeria and their transfer to a new genus, "Murraya," as "Murraya grayi subsp. gruyi" and "Murraya grayi subsp. murrayi" (22). DNA-The close relationship between these two species has been shown by numerical taxonomic analyses, which grouped them as a single distinct cluster with a level of similarity of 85 to 90% (6, 26), as well as by multilocus enzyme analysis (2). Furthermore, the sharing of specific chemotaxonomic properties distinguish these organisms from other members of the genus Listeria and support their close relationship; they have the same DNA base composition values (6, 21), produce similar electropherograms for whole-cell proteins (lo), and exhibit identical substitutions of the lipoteichoic acids (16) and a common antigenic structure (17), with small differences in 0 factors (23). Finally, the two species have been distinguished from each other only on the basis of nitrate reduction data (18).In this study we thoroughly reexamined the genomic distance between L . grayi and L . murrayi in order to evaluate whether these two taxa should be retained. To do this, we used the following three methods: DNA-DNA hybridization, multilocus enzyme electrophoresis, and rRNA gene restriction fragment length polymorphism. * Corresponding author. MATERIALS AND METHODSBacterial strains. The designations and sources of the five strains of L . grayi and the five strains of L . murrayi used in this study are shown in Table 1. These organisms were identified by using well-recognized biochemical markers (18).DNA-DNA hybridization. DNA-DNA hybridization experiments were performed as previously described (13), except that we used the procedure described below to lyse the bacteria. The growth from six Roux flasks that were incubated for 48 h at 37°C and contained 150 ml of Columbia agar was harvested, washed in 20 ml of 0.1 x SSC ( l x SSC is 0.15 M NaCl plus 0.015 M trisodium citrate, pH 7.0), and incubated for 1 h at 37°C in 5 ml of a lysozyme solution (0.01 M sodium phosphate-20% sucrose [pH 71 containing 12 mg of lysozyme [Applighe, France]). Then we added 40 ml of a proteinase K solution (10 mM Tris-HC1 [pH 81, 1 mM EDTA, 1% sodium dodecyl sulfate, 20 mg of proteinase K [Appligihe]), and the mixture was incubated for 2 h at 37°C. The DNA wa...

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“…rDNA fingerprinting is used increasingly for classification (7,8,12). Our results provided further evidence of the high level of homology between L .…”

Section: Resultssupporting

confidence: 72%

Assignment of Listeria grayi and Listeria murrayi to a Single Species, Listeria grayi, with a Revised Description of Listeria grayi

Rocourt

Boerlin

Grimont

et al. 1992

International Journal of Systematic Bacteriology

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“…In contrast, ribopatterns based on rRNA gene restriction patterns are comprised of fewer, more discrete bands and offer greater potential as a means of 'typing' isolates of H. pylori [10,11]. The technique has been applied to an increasing number of bacterial species [25]. In Campylobacter jejuni, for example, discrimination with ribopatterns was comparable to that achieved by phage typing and was superior in some respects to standard serotyping methods [26].…”

Section: Discussionmentioning

confidence: 99%

Ribosomal RNA gene patterns ofHelicobacter pylorifrom surgical patients with healed and recurrent peptic ulcers

Owen¹,

Bickley²,

Lastovica

et al. 1992

Epidemiol. Infect.

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SUMMARYFifty-two strains of Helicobacter pylori were examined by DNA restriction endonuclease digestion, ribosomal (r)RNA gene probe hybridization and biotyping. Most (49) strains originated from gastric (antral) biopsies of patients before or after elective surgery for duodenal ulcers. Chromosomal DNA Hind III ribopatterns showed 9 strain clusters of which the largest contained 12 strains each with 3 common bands (1 50, 3 45, and 4 26 kb) but which were heterogeneous with respect to biotype and total digest pattern. Isolates from post-operative patients with either healed or recurrent ulcers showed ribopattern heterogeneity and exhibited a similar distribution of H. pylori ribopattern types; no single type predominated in any patient group or was more highly associated with recurrent ulcers than with healed ulcers. Multiple isolates from two surgical patients had only minor genomic variations in each set whereas isolates from two brothers had different ribopatterns. We conclude that Hind III ribopatterns in conjunction with total digest patterns might provide the basis for future epidemiological typing studies.

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“…Also, as expected, PS 95 and PS 42E were the most distinct, with strains of phage groups I and III showing a greater degree of similarity to each other than either did to strains of group II or group V. PS 94 and PS 96, the propagating strains of the group V phages were related to PS 3A and the 85 % level and to the remainder of the group II phages at 75 % level. DISCUSSION Ribotyping is a method of genotyping which uses a probe derived either directly from cloned r-RNA genes or labelled cDNA to 168 + 238 r-RNA [15]. The r-RNA used may be homologous or heterologous.…”

Section: Ribotypingmentioning

confidence: 99%

“…The discriminatory ability of a genotyping method, ribotyping [15] was assessed using the propagating strains of the international phages of S. aureus as the test series. It was hoped that testing this collection would confirm the genetic distance of strains of phage group II or V, the well-defined restriction groups, from strains of the less well defined groups 1, III or I +111.…”

Section: Introductionmentioning

confidence: 99%

Ribotyping ofStaphylococcus aureus: an assessment using well–defined strains

Richardson

Aparicio²,

Marples³

et al. 1994

Epidemiol. Infect.

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SUMMARYRibotyping, with homologous or heterologous (Escherichia coli) r-RNA, of the propagating strains for phages of the international set for strains of Staphylococcus aureus of human origin was undertaken to determine the discrimination of this typing method. Ribotyping could distinguish between strains of different phage groups, but could not distinguish between seven phage group III strains of different phage type. Ribotyping may be a useful adjunct to phage typing in S. aureus but is unlikely to replace it as the primary method of epidemiological typing.

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Chromosomal DNA fingerprinting--a new method of species and strain identification applicable to microbial pathogens

Cited by 127 publications

References 27 publications

Assignment of Listeria grayi and Listeria murrayi to a Single Species, Listeria grayi, with a Revised Description of Listeria grayi

Assignment of Listeria grayi and Listeria murrayi to a Single Species, Listeria grayi, with a Revised Description of Listeria grayi

Ribosomal RNA gene patterns ofHelicobacter pylorifrom surgical patients with healed and recurrent peptic ulcers

Ribotyping ofStaphylococcus aureus: an assessment using well–defined strains

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