Chromosomal heterochromatin differentiation in North American cyprinid fishes.

Gold, John R.; Amemiya, Chris T.; Ellison, J. R.

doi:10.1508/cytologia.51.557

Cited by 38 publications

(25 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There were at least 200 fish species treated with Ag-NOR staining technique. Some case studies were applied to support the taxonomy of fish morphology with high similarity, such as the report of Gold et al (1986) who studied the cytogenetic of the family Cyprinidae. Moreover, the amount and location of NOR can explain the evolution of each chromosome.…”

Section: Resultsmentioning

confidence: 99%

A New Natural Autotetraploid and Chromosomal Characteristics of Dwarf Snakehead Fish, Channa gachua (Perciformes, Channidae) in Thailand

et al. 2014

View full text Add to dashboard Cite

Summary A new natural autotetraploid and the chromosomal characteristics of the dwarf snakehead fish (Channa gachua) from Kalasin, Nong Khai, Nong Bua Lam Phu and Udon Thani Provinces (four populations) in northeast Thailand were studied. Kidney cell samples were taken from 12 male and 12 female fish. Mitotic chromosome preparations were conducted using a blood cell culture technique as well as taken directly from kidney cells. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that the autotetraploid chromosome number of C. gachua was 4n = 104, and the fundamental number (NF) was 112 in both sexes. The types of chromosomes were 4 large submetacentric, 2 large acrocentric, 16 large telocentric, 2 medium submetacentric, 68 medium telocentric and 12 small telocentric chromosomes. No strange-sized chromosomes related to sex were observed. The region adjacent to the centromere of chromosome pair 3 showed clearly observable secondary constriction (NORs). The karyotype formula for C. gachua is as follows:4n (

show abstract

Section: Resultsmentioning

confidence: 99%

A New Natural Autotetraploid and Chromosomal Characteristics of Dwarf Snakehead Fish, Channa gachua (Perciformes, Channidae) in Thailand

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Variations in nuclear DNA content within a species Diploid Grass carp (7) Golden pheasant (20) Domestic chicken (25) Triploid Grass carp (14) Moluccan cockatoo ( 2 ) Common carp ( 5 ) Canebrake rattlesnake (1) Paddlefish (7) Northern watersnake (3) Sea lamprey (10) Hardhead catfish (1) Red-eared slider turtle (11) Spiny soft-shelled turtle (17) Pacific hagfish (12) Rainbow trout (9) Eastern box turtle (I) Domestic dog (2) Brown trout (3) Domestic swine (20) Domestic horse ( 5 ) Human (50) Domestic cattle ( 5 ) Northern cricket frog (2) Opossum (1) American toad (6) Fowler's toad (4) Atlantic stingray (1) Bullfrog ( 5 ) Marbled salamander (6) Red-spotted newt (2) Spotted salamander (4) 'This was a mean value; nine species of Xiphophorus were studied (16 stocks); among the different stocks DNA estimates varied from 1.48 pg to 1.56 pg (26).…”

Section: Discussionmentioning

confidence: 99%

Reference standards for flow cytometry and application in comparative studies of nuclear DNA content

et al. 1989

View full text Add to dashboard Cite

Flow cytometry can be used to obtain high-resolution estimates of nuclear DNA content (8,12,27). Much of the work in this area has been confined to clinical studies in the human, but the technology has been extended to organisms such as pocket gophers (241, triploid trout ( 2 3 , side-necked turtles (61, sex-reversed horses (16), and oysters (1).In these and similar studies, the DNA content of target cells is quantified relative to a standard DNA content in cells from a reference species (13,14,28). Standards may be used as internal references, when target cells and reference cells are mixed together and assayed simultaneously, or as external references, when target cells and cells from the reference species are analyzed independently. When external references are used, the flow cytometer must be checked for equilibration between analyses.The standard can be used for several calculations. The standard can be assigned a known DNA mass, against which picogram quantities of nuclear DNA from target cells can be estimated directly (15). Alternatively, a standard could be used as an internal reference in the analysis of individual samples, following which the reference is cancelled during estimation of a DNA Index. An internal reference would also be cancelled during the calculation of picogram quantities of nuclear DNA relative to a separate standard that has a known DNA mass (and a known relationship to the internal reference). These last two calculations do not require knowledge of the precise DNA mass of the internal reference.Because the use of reference standards provides a relative measure of DNA content, it is crucial that the reference cells and the target cells have a similar DNA content to minimize possible zero shift error (28) and, in the case of a n internal reference, that the values for the reference and target cells do not overlap.In order to gather information relating to the selection of reference standards for use in flow cytometry, we have surveyed a wide spectrum of taxa representing each of the major vertebrate classes. Here we present the results of our survey in 45 species. These species were selected because of their broad distribution and general accessibility, and because they provide an array of fluorescence peaks spanning the range of DNA masses that might be encountered in comparative studies. ~~~

show abstract

“…Moreover, the amount and location of NORs can explain the evolution of each chromosome (Gold et al 1986). Normally, most fishes have only 1 pair of small NORs in a chromosome complement.…”

Section: Chromosome Marker Of O Bimaculatusmentioning

confidence: 99%

Standardized Karyotype and Idiogram of Two-Spot Glass Catfish, Ompok bimaculatus (Siluriformes, Siluridae) in Thailand by Conventional and Ag-NOR Staining Techniques

et al. 2012

View full text Add to dashboard Cite

Summary Standardized karyotype and idiogram of the two-spot glass catfish (Ompok bimaculatus) from Chi basin, Maha Sarakham Province, northeast Thailand, were studied. Kidney cell samples were taken from 5 male and 5 female fish. The mitotic chromosome preparations were prepared directly from kidney cells. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that the diploid chromosome number of O. bimaculatus was 2n=50, the fundamental number (NF) was 90 in both males and females. The types of chromosomes were 10 large metacentric, 2 large submetacentric, 4 large acrocentric, 2 large telocentric, 4 medium metacentric, 18 medium submetacentric, 2 medium acrocentric, 2 medium telocentric, and 6 small telocentric chromosomes. The region adjacent to telomere of the short arm of submetacentric chromosome pair 4 showed clearly observable nucleolar organizer regions (NORs

show abstract

Chromosomal heterochromatin differentiation in North American cyprinid fishes.

Cited by 38 publications

References 49 publications

A New Natural Autotetraploid and Chromosomal Characteristics of Dwarf Snakehead Fish, <i>Channa gachua</i> (Perciformes, Channidae) in Thailand

A New Natural Autotetraploid and Chromosomal Characteristics of Dwarf Snakehead Fish, <i>Channa gachua</i> (Perciformes, Channidae) in Thailand

Reference standards for flow cytometry and application in comparative studies of nuclear DNA content

Standardized Karyotype and Idiogram of Two-Spot Glass Catfish, <i>Ompok bimaculatus</i> (Siluriformes, Siluridae) in Thailand by Conventional and Ag-NOR Staining Techniques

Contact Info

Product

Resources

About