Chromosomal high mobility group (HMG) proteins of the HMGB-type occurring in the moss Physcomitrella patens

Kiilerich, Bruno; Stemmer, Christian; Merkle, Thomas; Launholt, Dorte; Gorr, Gilbert; Grasser, Klaus D.

doi:10.1016/j.gene.2007.09.025

Cited by 9 publications

(8 citation statements)

References 65 publications

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“…Transformed protoplasts were analysed by confocal laser scanning microscopy. We have used constructs that served as controls 28,29 in this experiment: GFP that is found in both cytosol and nucleus (Fig. 3b), and NLS-CHSDsRed that localises to the nucleus (Fig.…”

Section: Resultsmentioning

confidence: 99%

Transcript Elongation Factor TFIIS Is Involved in Arabidopsis Seed Dormancy

Grasser

Kane

Merkle

et al. 2009

Journal of Molecular Biology

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101

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Section: Resultsmentioning

confidence: 99%

Transcript Elongation Factor TFIIS Is Involved in Arabidopsis Seed Dormancy

Grasser

Kane

Merkle

et al. 2009

Journal of Molecular Biology

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101

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“…The HMG proteins are divided into three families including HMG-AT-hook families (HMGA), HMG-box families (HMGB), and HMGnucleosome binding families (HMGN) according to the the structure of their DNA binding domains as well as their substrate binding specificity [8,9]. Additionally, HMGB proteins are subdivided into HMGB1, HMGB2, and HMGB3, all of which contain two HMG-box domains and a highly acidic Cterminal tail [10,11]. The current study found the expression levels of HMGB1 gene were significantly higher than that of the corresponding normal tissues in a variety of tumor tissues, including colorectal cancer, breast cancer, pancreatic cancer, melanoma, et al, which suggested that HMGB1 was involved in the occurrence and development of tumors [12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Interference with HMGB1 increases the sensitivity to chemotherapy drugs by inhibiting HMGB1-mediated cell autophagy and inducing cell apoptosis

Zhang

Wang

et al. 2015

Tumor Biol.

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Non-small cell lung cancer is commonly seen with higher morbidity and mortality. High-mobility group protein 1 (HMGB1) is a highly conserved nuclear protein, which is involved in multiple human diseases including cancers. However, the mechanisms of HMGB1 in non-small cell lung cancer remain unclear. The goal of the present study is to identify the relationship between HMGB1 and the progresssion of non-small cell lung cancer and investigate the molecular mechanism of HMGB1 in non-small lung cancer cell lines. Firstly, we detected the expression levels of HMGB1 by by real-time PCR and western blotting analysis, and the results demonstrated that HMGB1 was much higher expressed in non-small cell lung cancer cell lines, including A549, SPC-1-1, NCI-2170, SK-MES-1, and NCI-H1299, compared with that of WI-38. Next, 5 μM of adriamycin (AMD), 20 μM of cisplatin (DDP), and 50 μM of methotrexate (MTX) were used to treat A549 cells and SPC-A-1 cells for 48 h. The results showed that treatment with chemotherapy drugs significantly increased the levels of HMGB1 in A549 cells and SPC-A-1 cells. Moreover, the expression levels of HMGB1 increased in a time-dependent manner being treated with DDP. Then, the endogenous HMGB1 expression was successfully interferred with shRNA specific to HMGB1 in A549 and SPC-A-1 cells, which was detected by western blotting analysis. Then, the cisplatin-sensitive A549 cells and cisplatin-resistant A549/DDP cells were treated with increasing concentrations of cisplatin for 24, 48, and 72 h; cell viability were analyzed by MTT assay; and IC50 values were calculated. The results demonstrated that the expression level of HMGB1 in A549/DDP cells was much higher than that of A549 cells; moreover, transfection with HMGB1 shRNA in A549/DDP cells decreased the IC50 value of cisplatin in A549/DDP cells. The expression levels of autophagy-related proteins beclin-1 and LC3-II were significantly higher in A549/DDP cells or the A549 cells treated with chemotherapeutic drugs, compared with that in A549 cells. However, interference with endogenous HMGB1 obviously suppressed autophagy-related proteins and increased cell apoptosis rate and the expression of cleaved caspase-3 in A549/DDP cells. All of the data suggested that interference with the endogenous HMGB1 significantly inhibited cell autophagy and increased cell apoptosis of A549/DDP cells. Thus, the study on the resistance of chemotherapy drugs would provide a theoretical reference for clinical treatment of non-small cell lung cancer.

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“…LINAN ZHANG ET AL. characterized in a variety of animals (Watson et al 1977, Claus et al 1994, Bustin 1999, fungi (Weber and Isenberg 1980), higher plants (Stemmer et al 1997, 1999, Ritt et al 1998, Grasser et al 2006, and lower plants (Kiilerich et al 2008). As a type of DNA-binding protein, HMG-box proteins are involved in various functions, including modulating the chromatin structure and genomic stability, as well as contributing to the regulation of differentiation and development (Thomas 2001, Ueda andYoshida 2010).…”

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confidence: 99%

Isolation and expression analysis of a candidate gametophyte sex determination gene (sjhmg) of kelp (Saccharina japonica)

Zhang

et al. 2019

Journal of Phycology

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Saccharina japonica undergoes an alternating life cycle during which the diploid sporophyte generation alternates with the happloid gametophyte generation. Saccharina japonica uses the UV sex determination system to determine the sex of its haploid gametophytes. However, the sex‐determining genes and the sex‐determining mechanisms of kelp gametophytes have not been thoroughly elucidated to date. In this study, a kelp HMG‐box‐containing gene (SjHMG), which is located within the sex determination region of S. japonica, was isolated and characterized. SjHMG contained an open reading frame of 1,266 bp in length and encoded a deduced protein of 421 amino acid residues with two HMG‐box domains. Phylogenetic analysis showed the strongest relationship between SjHMG and its orthologs in brown algae. An alternatively spliced transcript (SjHMG isoform‐2) encoding a protein of 256 amino acid residues was also identified. The two isoforms were specific for male gametophytes. A real‐time quantitative PCR analysis showed significantly higher abundances of two isoforms in immature male gametophytes than in mature ones. These findings suggested that the SjHMG gene is a candidate male gametophyte determination gene of kelp. In addition, the abundance of SjHMG isoform‐2 transcripts was significantly lower than that of SjHMG isoform‐1 transcripts, and only an HMG‐box domain was conserved among species in the order Laminariales, which indicated that the gene is specifically involved in sex regulation in some species of the order Laminariales by alternative splicing.

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Chromosomal high mobility group (HMG) proteins of the HMGB-type occurring in the moss Physcomitrella patens

Cited by 9 publications

References 65 publications

Transcript Elongation Factor TFIIS Is Involved in Arabidopsis Seed Dormancy

Transcript Elongation Factor TFIIS Is Involved in Arabidopsis Seed Dormancy

Interference with HMGB1 increases the sensitivity to chemotherapy drugs by inhibiting HMGB1-mediated cell autophagy and inducing cell apoptosis

Isolation and expression analysis of a candidate gametophyte sex determination gene (sjhmg) of kelp (Saccharina japonica)

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