Chromosomal Localization of 5S rRNA Genes in Vicia faba and Crepis capillaris.

Hizume, Masahiro

doi:10.1508/cytologia.58.417

Cited by 51 publications

(42 citation statements)

References 28 publications

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“…We used EcoRI-digested plasmid with inserted wheat 35S rDNA (pTa71; Gerlach and Bedbrook 1979) and 5S rDNA amplified by PCR using the primers 5Sl1 (CCA TCA GAA CTC CGC AGT TA) and 5Sl2 (CGG TGC ATT AAT GCT GGT AT) (Hizume 1993) from Rumex acetosa genomic DNA. EcoRIdigested wheat 35S rDNA is generally identified as 45S rDNA, but identified here as 35S rDNA following the description of Garcia and Kovarik (2013).…”

Section: Fluorescence In Situ Hybridisationmentioning

confidence: 99%

A Comparative Analysis of Multi-Probe Fluorescence In Situ Hybridisation (FISH) Karyotypes in 26 Pinus Species (Pinaceae)

2016

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Summary Every species has a unique karyotype, but certain genera have common karyptypes among species. The markers (chromosome length and centromere position) used in traditional karyotyping do not distinguish all chromosome pairs in the genus Pinus. However, the application of multi-probe fluorescence in situ hybridisation (FISH) procedures allowed exact karyotyping of 26 Pinus congeners. We used these new data to examine species relationships. The 35S rDNA and 5S rDNA, Arabidopsis-type telomere repeat sequences, and the proximal CMA band-specific repeat (PCSR) of P. densiflora were used as FISH probes for our analysis of chromosomes in 26 Pinus species. Each species had a unique FISH karyotype and most homologous chromosome pairs were identified. The FISH karyotypes were used to compare corresponding or homologous chromosomes among the species. Common or similar FISH signal patterns appeared in closely related species. Species that had inherited common FISH signal patterns were classified into four karyotype groups. We used cluster analyses to compare quantitative differences in FISH signals within these groups. The results of these analyses were consistent with recent systematic interpretations and resolved differences among existing taxonomic systems based on diverse methodologies. Our results indicate that FISH signal patterns reflect the history of species differentiation and that comparative FISH karyotyping has potential as an important tool for studying the taxonomy or phylogeny of Pinus.

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Section: Fluorescence In Situ Hybridisationmentioning

confidence: 99%

A Comparative Analysis of Multi-Probe Fluorescence In Situ Hybridisation (FISH) Karyotypes in 26 Pinus Species (Pinaceae)

2016

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“…'Borszczagowski' by using polymerase chain reaction (PCR). The 18S rDNA fragment was amplified according to Sogin et al (1990), and 5S rDNA fragment according to Hizume et al (1993). For FISH experiments, 18S rDNA probe was labeled by using BioNick labeling system (Invitrogen), and 5S rDNA probes were labeled by PCR labeling method using 0.2 mM dATP, dGTP, dCTP, 0.1 mM dTTP and 0.1 mM tetramethylrhodamine-5-dUTP (Roche) as dNTP mixture.…”

Section: Plantmentioning

confidence: 99%

Cytogenetic comparison among three cultivars of cucumber (Cucumis sativus L.) by using post-heated DAPI band, 45S and 5S rDNA sites

et al. 2009

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ABSTRACT. The positions of heterochromatic bands, 45S and 5S rDNA regions were detected on the chromosomes in three cultivars of cucumber (Cucumis sativus L.). The post-heated DAPI bands, which revealed heterochromatic region, were observed on the both terminal and pericentromeric regions of most of the chromosomes in three cultivars. However, these band patterns were different between European and Eastern Asian cultivars. The major 45S rDNA sites were found around the centoromeric region of the chromosomes 1, 2 and 5, and the minor 45S rDNA sites were found around centoromeric region of the chromosomes 3 and 7. The positional variation of this region was not found among three cultivars, but size difference was observed in the chromosome 5. The 5S rDNA site was observed on the interstitial region of the chromosome 6 in all of the cultivars studied. These results indicated that the variation of chromosome structure among cucumber cultivars occurred in the heterochromoatic region and suggested that the genetic variation in cucumber cultivars would be revealed by strict analysis and comparison for the position and size of heterochromatic region.

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“…PCR amplification of 5S and 18S rDNAs followed the procedure of Hizume (1993) and Sogin (1990), respectively. The sequences of the primers were as follows: 5Ј-CGGTGCATTAATGCTGGTAT-3Ј and 5Ј-CCATCAGAACTCCGCAGTTA-3Ј for the repeating units in 5S rRNA gene clusters, and 5Ј-AACCTGGTTGATCCTGCCAGT-3Ј and 5Ј-TGATC-CTTCTGCAGGTTCACCTAC-3Ј for the 18S rRNA coding regions.…”

Section: Pcr Amplification Of 5s And18s Ribosomal Dnas and Arabidopsmentioning

confidence: 99%

Physical Mapping of 5S rDNA, 18S rDNA and Telomere Sequences in Three Species of the Genus Artemisia (Asteraceae) with Distinct Basic Chromosome Numbers

Matoba

Uchiyama

2009

CYTOLOGIA

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Summary Fluorescence in situ hybridization (FISH) with both of 5S and 18S rDNAs, and Arabidopsis-type telomere sequences was carried out in 3 species of the genus Artemisia (Asteraceae) with different basic chromosome numbers, xϭ8, 9 and 17, to understand their chromosomal organization and evolution. Chromosomal distribution of telomere sequences, which was restricted only to the ends of all chromosomes, was reported here for the first time in this genus. Although 5S rRNA gene loci are generally localized independently of 45S rRNA gene loci in eukaryotic chromosomes, all of the 5S rDNA sites are the same number and co-localized with the 18S rDNA sites in the species investigated. We also discussed the chromosomal evolution of A. feddei (xϭ8) and A. princeps (xϭ17) having a derivative basic chromosome number in this genus.

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Chromosomal Localization of 5S rRNA Genes in Vicia faba and Crepis capillaris.

Cited by 51 publications

References 28 publications

A Comparative Analysis of Multi-Probe Fluorescence <i>In Situ</i> Hybridisation (FISH) Karyotypes in 26 <i>Pinus</i> Species (Pinaceae)

A Comparative Analysis of Multi-Probe Fluorescence <i>In Situ</i> Hybridisation (FISH) Karyotypes in 26 <i>Pinus</i> Species (Pinaceae)

Cytogenetic comparison among three cultivars of cucumber (Cucumis sativus L.) by using post-heated DAPI band, 45S and 5S rDNA sites

Physical Mapping of 5S rDNA, 18S rDNA and Telomere Sequences in Three Species of the Genus Artemisia (Asteraceae) with Distinct Basic Chromosome Numbers

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