Chromosomal localization of actin genes in the malaria mosquito Anopheles darlingi

Abstract

Physical and genetic maps have been used for chromosomal localization of genes in vectors of infectious diseases. The availability of polytene chromosomes in malaria mosquitoes provides a unique opportunity to precisely map genes of interest. We report physical mapping of two actin genes on polytene chromosomes of the major malaria vector in Amazon Anopheles darlingi. The clones with the actin genes sequences were obtained from a cDNA library constructed from RNA isolated from adult females and males of An. darlingi. Each of the two clones was mapped to a unique site on the chromosomal arm 2L in subdivisions 21A (clone pl05-A04) and 23B (clone pl17-G06). The obtained results together with previous mapping data provide a suitable basis for comparative genomics and for establishing chromosomal homologies among major malaria vectors.

Identification and validation of the first EST-SSR markers based on transcriptome of Anopheles darlingi, the primary transmitter of malaria in Brazil

“…These gene sequences showed regions of similarity in the 2L, 2R, 3L and X chromosome arms between both species, as well as with ESTs from other insects such as Aedes aegypti, Drosophila melanogaster and Culex spp (L. Bridi, personal communication). Furthermore, troponin (L. Bridi, personal communication) and actin [25] were hybridized as probes via a uorescent in situ hybridization method in the polytene chromosomes of A. darlingi, which showed markers located in the arm 2R, section 14B, and in the arm 2L, section 23, respectively. These results provide a suitable basis for establishing chromosomal and evolutionary genomics homologies among major malaria vectors.…”

A look into the Medical and Veterinary Entomology crystal ball

“…, ; Bridi et al. , ). However, there are several other areas in which greater attention from medical and veterinary entomologists is required and further studies on these topics are advocated.…”

Physical Mapping of the Anopheles (Nyssorhynchus) darlingi Genomic Scaffolds

“…darlingi chromosomes. Positions of gene-specific sequences for rDNA [ 14 ], heat shock protein (Hsp) 70 [ 15 ], actin [ 16 ], myosin [ 17 ], glutathione S transferase (GST) [ 8 ], and Gram-negative bacteria binding protein (GNBP) [ 18 ] have been placed onto the An. darlingi photomap [ 19 ].…”