Chromosomal localization of nine porcine genes encoding transcription factors involved in adipogenesis

Szczerbal, Izabela; Chmurzyńska, Agata

doi:10.1159/000124381

Cited by 10 publications

(7 citation statements)

References 48 publications

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“…We selected nine genes-seven of which are involved in adipogenesis (PPARG, SREBF1, CEBPA, CEBPB, CREB, FABP4, GATA2) and two that were used as control genes (SOX9, MYL1; Table 1). The genes were mapped by FISH to different porcine chromosomes, such as SSC4, SSC6, SSC12, SSC13, SSC15, and SSC17 (Szczerbal and Chmurzynska 2008;Szczerbal et al 2007a, b). We selected the genes so that adipogenic and control genes or pro-and anti-adipogenic genes were located on the same chromosome (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…BAC probes for adipogenic genes were selected based on previous information about the gene localization (Szczerbal and Chmurzynska 2008;Szczerbal et al 2007a, b). BAC probes for control genes (MYL1, SOX9) were used for the first time in this study and were verified by PCR using specific primers (primer sequences available on request) and by fluorescence in situ hybridization (FISH) on metaphase chromosomes.…”

Section: Dna Probesmentioning

confidence: 99%

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The spatial repositioning of adipogenesis genes is correlated with their expression status in a porcine mesenchymal stem cell adipogenesis model system

2009

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Alterations in the nuclear positioning of chromosomes and specific genes during differentiation and development have suggested strongly the existence of a relationship between non-random organization of the genome and its function. In this study, we have examined the genome organization in interphase nuclei during adipogenesis, using the pig as a model organism. We hypothesized that changes in the gene expression profile and chromatin remodeling which occur during cellular differentiation would elicit repositioning of whole chromosomes, moving specific genes on them to different regions of the nucleus. We established an in vitro adipogenesis differentiation system using mesenchymal stem cells, derived from porcine bone marrow. The nuclear position of seven adipogenesis genes (PPARG, SREBF1, FABP4, CEBPA, CEBPB, CREB, and GATA2), two control genes (SOX9 and MYL1), and six chromosomes carrying these gene loci (SSC4, SSC6, SSC12, SSC13, SSC15, and SSC17) was determined. We found that during adipogenesis, using the in vitro stem cell model system, in contrast to our original hypothesis, the nuclear position of genes involved in adipogenesis was altered radically with the up-regulation of gene expression correlating with these genes becoming more internally located within nuclei. Chromosome territories, containing these genes, were also found to alter their nuclear position during the in vitro adipogenesis model, with the most dramatic repositioning being SSC4 that moved from the nuclear periphery towards the nuclear interior. We found that during in vitro adipogenesis chromosome territories decondensed and the genes were found on loops and projections of chromatin, away from the main body of the chromosomes. From our data, it appears that the temporal repositioning of genes, emanating away from chromosomes, during adipogenesis is correlated with gene activity, supporting models of the involvement of spatial genome repositioning in regulating gene expression and the nuclear interior being an important region of the nucleus for transcription.

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Section: Resultsmentioning

confidence: 99%

Section: Dna Probesmentioning

confidence: 99%

The spatial repositioning of adipogenesis genes is correlated with their expression status in a porcine mesenchymal stem cell adipogenesis model system

2009

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“…ACACA and SREBF1 , were localized in SSC12. The chromosomal location of SREBF1 was already shown earlier, mapping to SSC12q15 [Szczerbal and Chmurzynska, 2008], while ACACA was assigned to SSC12q12-q13. The location of ACACA is inconsistent with a previous mapping report [Calvo et al, 2000] in which the authors indicated the location in the p-arm of SSC12.…”

Section: Resultsmentioning

confidence: 52%

Three-Dimensional Arrangement of Genes Involved in Lipid Metabolism in Nuclei of Porcine Adipocytes and Fibroblasts in Relation to Their Transcription Level

Kociucka

Cieślak²,

Szczerbal³

2012

Cytogenet Genome Res

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The 3-dimensional arrangement of chromosomes and genes within a nuclear space is considered to represent the level of transcriptional regulation. Understanding how the nuclear architecture of adipocyte cells contributes to gene expression has become the subject of greatinterest in the context of obesity research. In this study we investigated nuclear positioning of 3 gene loci involved in lipid metabolism in the pig (Sus scrofa, SSC) which is considered as an important animal model for obesity in humans. We found that the position of the SCD gene in the 3-dimensional space of the cell nucleus is not correlated with transcriptional activity. The gene locus as well as chromosome territory SSC14 occupied the same peripheral location in adipocyte and fibroblast cells, in spite of the fact that their transcription level differs significantly between both cell types. For the 2 other investigated genes, i.e. ACACA and SREBF1 and their chromosome territory (SSC12), slightly different nuclear locations were found. They occupied intermediate nuclear positions in fibroblast nuclei, while in adipocytes they were positioned in the nuclear interior. The more internal location of these genes corresponds to increased transcription levels in fat cells. Our results confirm the non-random position of genes and chromosome territories in nuclei of adult porcine cells and indicate that relationship between transcription activity and gene positioning exists only for some but not all genes.

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“…For example, porcine candidate genes, including nine genes encoding adipogenic transcription factors (174), are widely distributed among porcine chromosomes (174). Twenty-five candidate genes are localized on chromosomes 1, 2, 4-7, 9, 10, 12, 13, 17, and 18, with chromosome 6 containing 7 genes, whereas many other chromosomes contain one or two (174). It will be important to demonstrate which of the candidate genes are critical players by demonstrating functionality that results in a particular phenotype.…”

Section: Identification Of Genes Influencing Adipose Tissue Differentmentioning

confidence: 99%

Preadipocyte and Adipose Tissue Differentiation in Meat Animals: Influence of Species and Anatomical Location

Hausman¹,

Basu

Wei

et al. 2014

Annu. Rev. Anim. Biosci.

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Early in porcine adipose tissue development, the stromal-vascular (SV) elements control and dictate the extent of adipogenesis in a depot-dependent manner. The vasculature and collagen matrix differentiate before overt adipocyte differentiation. In the fetal pig, subcutaneous (SQ) layer development is predictive of adipocyte development, as the outer, middle, and inner layers of dorsal SQ adipose tissue develop and maintain layered morphology throughout postnatal growth of SQ adipose tissue. Bovine and ovine fetuses contain brown adipose tissue but SQ white adipose tissue is poorly developed structurally. Fetal adipose tissue differentiation is associated with the precocious expression of several genes encoding secreted factors and key transcription factors like peroxisome proliferator activated receptor (PPAR)γ and CCAAT/-enhancer-binding protein. Identification of adipocyte-associated genes differentially expressed by age, depot, and species in vivo and in vitro has been achieved using single-gene analysis, microarrays, suppressive subtraction hybridization, and next-generation sequencing applications. Gene polymorphisms in PPARγ, cathepsins, and uncoupling protein 3 have been associated with back fat accumulation. Genome scans have mapped several quantitative trait loci (QTL) predictive of adipose tissue-deposition phenotypes in cattle and pigs.

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Chromosomal localization of nine porcine genes encoding transcription factors involved in adipogenesis

Cited by 10 publications

References 48 publications

The spatial repositioning of adipogenesis genes is correlated with their expression status in a porcine mesenchymal stem cell adipogenesis model system

The spatial repositioning of adipogenesis genes is correlated with their expression status in a porcine mesenchymal stem cell adipogenesis model system

Three-Dimensional Arrangement of Genes Involved in Lipid Metabolism in Nuclei of Porcine Adipocytes and Fibroblasts in Relation to Their Transcription Level

Preadipocyte and Adipose Tissue Differentiation in Meat Animals: Influence of Species and Anatomical Location

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