Chromosomal location of a gene that controls sterol esterification in Triticum Aestivum L.

Torres, J.V.; Garcı́a-Olmedo, Francisco

doi:10.1016/0304-4211(74)90078-9

Cited by 9 publications

(11 citation statements)

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“…The present results show that the differences in sterol ester pattern controlled by gene Pin are confined to the endosperm and that the previously observed difference in kernel free sterol between euploid Chinese Spring wheat and lines lacking chromosome 7D [15], also exist between the PL and the L phenotype varieties and is equally restricted to the same tissue (Table I). The higher sterol level cosegregates with the L phenotype pattern (Table II), which means that both features are controlled by the same mendelian factor or by two closely linked factors.…”

Section: Discussionmentioning

confidence: 52%

“…Analysis of whole kernels of compensated nullitetrasomic and ditelosomic Unes of Triticum aestivum cv. Chinese Spring showed that gene Pin is located in the short arm of chromosome 7D [15]. Palmitate was found to be the dominant ester in all lines, except those lacking chromosome 7D, which had linoleate as the main ester.…”

Section: Introductionmentioning

confidence: 99%

“…The latter lines also showed a marked decrease in the esterified sterol of the whole kernel and an increase in free sterol. The presence of the short arm of chromosome 7D restored palmitate synthesis, increased the sterol ester level and decreased the free sterol [15].…”

Section: Introductionmentioning

confidence: 99%

“…The main sterol esters, palmitate, oléate and linoleate, and the free sterol were extracted, separated by preparative thin-layer chromatography, eluted and quantitated by fluorimetry as previously described [15].…”

Section: Analytical Proceduresmentioning

confidence: 99%

“…The characterization of genes affecting sterol esterification should contribute to clarify some of the above hypotheses. García-Olmedo and coworkers [12][13][14][15] have described a gene that controls the sterol ester pattern in the kernels of hexaploid wheat (Triticum aestivum L.). The PL phenotype pattern, which has palmitate as the main ester, is inherited as though determined by a dominant alíele at a single locus (Pin), while the linoleate pattern is inherited as a recessive alíele (pin) [14].…”

Section: Introductionmentioning

confidence: 99%

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Genetic control of sterol esterification in developing wheat endosperm

Torres

Garcı́a-Olmedo

1975

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metaboli

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Summary1. The action of gene Pin, previously characterized by the sterol ester patterns of mature whole wheat kernels, has been found to be restricted to the endosperm and not to affect the embryo, the pericarp or the seed coat.2. The dominant alíele Pin, which determines a sterol ester pattern with palmitate as the main ester, is also responsible for a low level of free sterol at maturity. A high level of free sterol is associated with the recessive alíele pin, which determines an ester pattern with linoleate as the main ester.3. Divergence between the two phenotypes starts at about 21 days after anthesis, when cell proliferation has been completed, the aleurone layer has differentiated, and only cell enlargement is taking place. A marked increase in esterification, mainly by palmitate, which is controlled by the dominant alíele, is concomitant with a sharp decrease in free sterol.4. The increased net esterification is non-specific with respect to 4-demethyl sterols, because it affects the four main ones, namely sitosterol, stigmasterol, campesterol and cholesterol.

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Section: Discussionmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Analytical Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Genetic control of sterol esterification in developing wheat endosperm

Torres

Garcı́a-Olmedo

1975

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metaboli

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Eyespot resistance gene Pch-1 from Aegilops ventricosa is associated with a different chromosome in wheat line H-93-70 than the resistance factor in “Roazon” wheat

Delibes¹,

Doussinault

Mena³

et al. 1988

Theoret. Appl. Genetics

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Summary. The hexaploid wheat line H-93-70 carnes a gene (Pch-1) that has been transferred from the wild grass Aegilops ventricosa and confers a high degree of resistance to eyespot diesease, caused by the fungus Pseudocercosporella herpotrichoides. Crosses of the resistant line H-93-70 with the susceptible wheat Pane 247 and with a 7D/7Ag wheat/Agropyron substitution line were carried out and F2 kernels were obtained. The kernels were cut transversally and the halves carrying the embryos were used for the resistance test, while the distal halves were used for genetic typing. Biochemical markers were used to discriminate whether the transferred Pch-1 gene was located in chromosome 7D, as is the case for a resistance factor present in "Roazon" wheat. In the crosses involving Pane 247, resistance was not associated with the 7D locus Pin, which determines sterol ester pattern (dominant alíele in H-93-70). In the crosses with the 7D/7Ag substitution line, resistance was neither associated with protein NGE-11 (7D marker), ñor alternatively inherited with respect to protein C-7 (7Ag marker). It is concluded that gene Pch-1 represents a different locus and is not an alíele of the resistance factor in "Roazon" wheat.

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