Chromosomal location of blaCTX-M genes in clinical isolates of Escherichia coli from Germany, The Netherlands and the UK

Rodríguez, Irene; Thomas, Katharina; Essen, Anthonie van; Schink, Anne Kathrin; Day, Michaela; Chattaway, Marie Anne; Wu, Guanghui; Mevius, Dik; Helmuth, Reiner; Guerra, Beatriz

doi:10.1016/j.ijantimicag.2014.02.019

Cited by 79 publications

(59 citation statements)

References 14 publications

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“…The K. pneumoniae isolate belonged to ST392, previously associated with the dissemination of bla KPC , bla , and other ESBLs (13,14). In addition, this isolate also harbored a chromosomal bla and bla CTX-M-15 , the latter located downstream of ISEcp1, as previously reported in other Enterobacteriaceae isolates from Spain, Japan, Germany, the Netherlands, and the United Kingdom (15)(16)(17)(18) 15 , and bla OXA-1 ). In this case, the second copy of bla CTX-M-15 was found to be part of a previously reported structure: a Tn3-like transposon also carrying bla TEM-1 which has its tnpA gene disrupted by ISEcp1-bla CTX-M-15 (19).…”

supporting

confidence: 57%

Initial Assessment of the Molecular Epidemiology of bla _NDM-1 in Colombia

Rojas

Wright

Cadena

et al. 2016

Antimicrob Agents Chemother

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fWe report complete genome sequences of four bla NDM-1 -harboring Gram-negative multidrug-resistant (MDR) isolates from Colombia. The bla NDM-1 genes were located on 193-kb Inc FIA, 178-kb Inc A/C2, and 47-kb (unknown Inc type) plasmids. Multilocus sequence typing (MLST) revealed that these isolates belong to sequence type 10 (ST10) (Escherichia coli), ST392 (Klebsiella pneumoniae), and ST322 and ST464 (Acinetobacter baumannii and Acinetobacter nosocomialis, respectively). Our analysis identified that the Inc A/C2 plasmid in E. coli contained a novel complex transposon (Tn125 and Tn5393 with three copies of bla NDM-1 ) and a recombination "hot spot" for the acquisition of new resistance determinants.A t this time, bla NDM is recognized as a major global health threat. Guatemala and Colombia reported the first cases of bla NDM-1 -harboring isolates in Latin America (1, 2). In both instances, bla NDM-1 was discovered in hospital-acquired, clonally related Klebsiella pneumoniae isolates that were recovered from patients who had not travelled recently. The molecular epidemiology of bla NDM carbapenemases in South America has been investigated only in a limited fashion, and data in Colombia are very scarce. In order to understand the dissemination of bla NDM-1 in Colombia, we performed a genomic analysis of four sentinel isolates, Acinetobacter baumannii, Acinetobacter nosocomialis, Escherichia coli, and K. pneumoniae collected in 2012, shortly after the first reported outbreak (1).

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supporting

confidence: 57%

Initial Assessment of the Molecular Epidemiology of bla _NDM-1 in Colombia

Rojas

Wright

Cadena

et al. 2016

Antimicrob Agents Chemother

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“…This resistant clone is not common in the Netherlands and is rare in Dutch hospitals. 22 Considering these facts, it is very likely that transmission within the NH occurred.The high prevalence of the ST131 clonal group and the large cluster size in our surveillance suggests that ST131 can spread very easily and is more prevalent in the Netherlands than is currently known. The rapid emergence and successful spread of E. coli ST131 is strongly associated with several factors, including the production of the CTX-M1-15 ESBL, as was the case in our surveillance.…”

mentioning

confidence: 99%

“…This resistant clone is not common in the Netherlands and is rare in Dutch hospitals. 22 Considering these facts, it is very likely that transmission within the NH occurred.…”

mentioning

confidence: 99%

Extensive Dissemination of Extended Spectrum β-Lactamase–Producing Enterobacteriaceae in a Dutch Nursing Home

Willemsen

Nelson

Hendriks

et al. 2015

Infect. Control Hosp. Epidemiol.

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objective. Risk factors for rectal carriage of ESBL-E and transmission were investigated in an outbreak of extended-spectrum β-lactamaseproducing Enterobacteriaceae (ESBL-E).design. Rectal carriage of ESBL-E was determined in a cross-sectional survey by culture of perianal swabs or fecal samples. Both phenotypical and genotypical methods were used to detect the production of ESBL. Nosocomial transmission was defined as the presence of genotypically related strains in ≥2 residents within the NH. Patient characteristics and variables in infection control practices were registered to investigate risk factors for transmission.setting. A nursing home (NH) in the southern Netherlands.participants. Of 189 residents, 160 residents (84.7%) were screened for ESBL-E carriage. Of these 160 residents, 33 (20.6%) were ESBL-E positive. ESBL carriage rates varied substantially between wards (range, 0-47%). Four different ESBL-E clusters were observed. A bla CTX-M1-15 positive E. coli ST131 constituted the largest cluster (n = 21) and was found in multiple wards (n = 7).results. Our investigation revealed extensive clonal dissemination of bla CTX-M1-15 -positive E. coli ST131 in a nursing home. Unexplained differences in ESBL prevalence were detected among the wards.conclusions. As NHs constitute potential sources of multidrug-resistant bacteria, it is important to gain a better understanding of the risks factors and routes of transmission of ESBL-E. 2015;36(4):394-400 Infections due to extended-spectrum β-lactamase producing Enterobacteriaceae (ESBL-E) are a major health concern worldwide. Infect Control Hosp Epidemiol1 Until the late 1990s, most ESBL-E were related to healthcare settings, typically Klebsiella pneumoniae, and predominantly carried mutant forms of the long-established TEM or SHV penicillinases. However, since the turn of the century, ESBL-Es have become widespread outside the hospital, and strains of Escherichia coli with CTX-M ESBL have become prominant. 2 The dissemination of some successful strains, such as E. coli sequence type ST131, often in the presence of CTX-M1-15 resistance genes, has been reported in association with healthcare settings and elderly hosts.3 Nursing homes (NHs) may become reservoirs of ESBL-producing Enterobacteriaceae (ESBL-E) because the residents frequently require medical care and antimicrobial therapy. 4,5 Residents colonized with ESBL-E in the gastrointestinal tract may serve as reservoirs for others. 6 Once resistance is present in an NH, resistant strains tend to persist and become endemic because the length of stay is much longer than in acute care hospitals and infection control practices are less stringent. 7A cross-sectional survey, performed in an NH in the southern Netherlands, revealed a high proportion of residents with rectal ESBL-E carriage. 8 The results of this survey were further investigated, and an outbreak of ESBL-E. coli sequence type ST131 was detected. We further investigated risk factors for rectal carriage of ESBL-E and transmission of the outbreak strain...

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“…The coexistence of CTX-M-14 and SHV-12 has been reported in Korea [40], and the coexistence of CTX-M-14 and CTX-M-15 has been reported in the U.S. [46]. The dissemination of bla CTX-M-27 typically occurs through plasmids of the IncF incompatibility group[47], whereas bla CTX-M-14 is found in the IncK and IncH12 plasmids [48,49], bla CTX-M-14 has been reported to be located in the chromosome [50]. CTX-M-27 can be differentiated from CTX-M-14 by the substitution of an amino acid, D240G.…”

Section: Discussionmentioning

confidence: 99%

“…Countries as China, Japan, Korea, Sweden, Netherlands and United Kingdom have been reported E . coli ST38 with the subtype CTX-M-14 in human infections [50, 55–59]. In Korea, ST38 was reported as the second most frequent ST in CTX-M-14 producing E .…”

Section: Discussionmentioning

confidence: 99%

First Report of Group CTX-M-9 Extended Spectrum Beta-Lactamases in Escherichia coli Isolates from Pediatric Patients in Mexico

Mérida-Vieyra

Colsa

Castañeda³

et al. 2016

PLoS ONE

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The aim of this study was to identify the presence of group CTX-M-9 extended spectrum beta-lactamases (ESBL) in clinical Escherichia coli isolates from pediatric patients. A total of 404 non-repeated positive ESBL E. coli isolates were collected from documented clinical infections in pediatric patients over a 2-year period. The identification and susceptibility profiles were determined using an automated system. Isolates that suggested ESBL production based on their resistance profiles to third and fourth generation cephalosporin and monobactam were selected. ESBL production was phenotypically confirmed using a diffusion method with cefotaxime and ceftazidime discs alone and in combination with clavulanic acid. blaESBL gene identification was performed through PCR amplification and sequencing. Pulsed Field Gel Electrophoresis (PFGE) and Multilocus Sequence Typing (MLST) were performed to establish the clonal relationships of the E. coli isolates. CTX-M-9-type ESBLs were detected in 2.5% of the isolates. The subtypes corresponded to blaCTX-M-14 (n = 4) and blaCTX-M-27 (n = 6). Additionally, coexistence with other beta-lactamases was observed. A clonal relationship was established in three isolates; the rest were classified as non-related. We found seven different sequence type (ST) in CTX-M-9- producing E. coli isolates. ST38 was the most frequent. This study is the first report in Mexico to document the presence of group CTX-M-9 ESBLs in E. coli isolates from pediatric patients.

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Chromosomal location of blaCTX-M genes in clinical isolates of Escherichia coli from Germany, The Netherlands and the UK

Cited by 79 publications

References 14 publications

Initial Assessment of the Molecular Epidemiology of bla _NDM-1 in Colombia

Initial Assessment of the Molecular Epidemiology of bla _NDM-1 in Colombia

Extensive Dissemination of Extended Spectrum β-Lactamase–Producing Enterobacteriaceae in a Dutch Nursing Home

First Report of Group CTX-M-9 Extended Spectrum Beta-Lactamases in Escherichia coli Isolates from Pediatric Patients in Mexico

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Chromosomal location of blaCTX-M genes in clinical isolates of Escherichia coli from Germany, The Netherlands and the UK

Cited by 79 publications

References 14 publications

Initial Assessment of the Molecular Epidemiology of bla NDM-1 in Colombia

Initial Assessment of the Molecular Epidemiology of bla NDM-1 in Colombia

Extensive Dissemination of Extended Spectrum β-Lactamase–Producing Enterobacteriaceae in a Dutch Nursing Home

First Report of Group CTX-M-9 Extended Spectrum Beta-Lactamases in Escherichia coli Isolates from Pediatric Patients in Mexico

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Initial Assessment of the Molecular Epidemiology of bla _NDM-1 in Colombia

Initial Assessment of the Molecular Epidemiology of bla _NDM-1 in Colombia