Chromosomal mapping of a locus associated with adult-stage resistance to powdery mildew from Agropyron cristatum chromosome 6PL in wheat

Lin, Yida; Zhou, Shumin; Liang, Xuezhong; Guo, Baojin; Han, Bing; Zhang, Jingpeng; Lu, Yuqing; Zhang, Zhi; Yang, Xinming; Li, Xiuquan; Liu, Weihua; Li, Lihui

doi:10.1007/s00122-022-04155-3

Cited by 12 publications

(4 citation statements)

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“…In our previous study, using deletion lines and translocation lines, we located many excellent genes from A. cristatum. For example, powdery mildew resistance genes and increased GNS genes were mapped to 6PL (0.27-0.51) by del27 and del16a (Lin et al 2022;Zhang et al 2019), leaf rust resistance genes and stripe rust resistance genes were mapped to 6PS (0.81-1.00) (Song et al 2016;Zhang et al 2017), and leaf rust resistance genes and powdery mildew resistance genes were mapped to 2PL (0.66-0.86) (Jiang et al 2018;Li et al 2017). Similarly, the blue-grain gene from Th.…”

Section: Discussionmentioning

confidence: 99%

“…The oligonucleotide probes Oligo-PTa535-1 (red) and Oligo-pSc119.2-1 (green) were used to detect wheat chromosomes by FISH analysis. The FISH procedure was performed according to the methods of Lin et al (2022). The translocated chromosomes and possible mutations within Fukuho were determined by comparisons with the Chinese Spring karyotype (Tang et al 2014).…”

Section: Gish and Fish Analysismentioning

confidence: 99%

“…Agropyron cristatum exists as diploid (2n = 2x = 14, PP), tetraploid (2n = 4x = 28, PPPP) and hexaploid (2n = 6x = 42, PPPPPP) forms, all of which have excellent high yield phenotypes (multiple orets, multiple spikelets, excellent fertility), stress tolerance (drought tolerance, cold tolerance, salt tolerance) and disease resistance (powdery mildew resistance, stripe rust resistance, leaf rust resistance) (Dong et al 2006;Zhou et al 2018;Lin et al 2022). In particularly, the 6P addition line 4844-12 (2n = 2x = 44) was generated by crossing the common wheat Fukuhokomugi (Fukuho) and A. cristatum (Z559), which resulted in a signi cant increase in GNS and TGW (Wu et al 2006;Zhang et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Introgression of Agropyron cristatum genes to counteract the negative relationship between grain number and grain weight of wheat

Lin

Zhou

Liang

et al. 2022

Preprint

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Agropyron cristatum (2n = 4X = 28, PPPP), which harbour many high-yield and disease-resistance genes, is a promising donor for wheat improvement. Narrow genetic diversity and the trade-off between grain weight and grain number have become bottlenecks for increasing grain yield. In this study, WAT650l, a new translocation line was generated via radiation (60Co-γ rays)-based mutation induction of chromosome 6P addition line 4844-12. Cytological analysis and molecular marker analysis revealed that WAT650l was a 6PL (bin ~ 12–17)·5BS-5BL translocation line. Assessment of agronomic traits and analysis of the BC4F2 and BC5F2 populations suggested that the 6PL terminal chromosome segment in WAT650l resulted in increased flag leaf length, plant height (PH), spikelet number per spike (SNS), kernel number per spikelet (KNS), grain number per spike (GNS) (average increased by 14.07 grains) and thousand-grain weight (TGW) (average increased by 4.31 g) during the growing seasons of 2020–2021 and 2021–2022. Additionally, the increased GNS locus and high-TGW locus of WAT650l were mapped to 6PL (16–17) and 6PL (12–13), respectively, by genetic population analysis of three translocation lines. In summary, by using high-yield genes of A. cristatum and overcoming the negative relationship between GNS and TGW associated with wheat breeding, we provide a valuable germplasm resource for broadening the genetic base of wheat.

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Section: Discussionmentioning

confidence: 99%

Section: Gish and Fish Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Introgression of Agropyron cristatum genes to counteract the negative relationship between grain number and grain weight of wheat

Lin

Zhou

Liang

et al. 2022

Preprint

Self Cite

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“…Powdery mildew (Pm), caused by Blumeria graminis f. sp. tritici (Bgt), is one of the destructive wheat diseases causing signi cant yield loss and deteriorated grain quality worldwide (Savary et al 2019, Lin et al 2022). The identi cation Pm resistance genes and their utilization in wheat breeding is the most effective and economical solution for Pm disease control.…”

Section: Introductionmentioning

confidence: 99%

Cytological mapping of a powdery mildew resistance locus PmRc1 by the construction of wheat-Roegneria ciliaris structural aberration library

Cheng,

Zhang,

Zhang

et al. 2023

Preprint

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Powdery mildew (Pm) is a fungal disease threatening wheat production. The identification of novel Pm resistance genes, especially those from wild relatives, will greatly broaden the genetic diversity of wheat breeding. Roegneria ciliaris (2n=4x=28, genome ScScYcYc) is a wheat relative. We developed 14 disomic addition lines in previous research. In this study, we identified addition line DA1Sc exhibited stable improved Pm resistance. We confirmed the Pm resistance was attributed by the added 1Sc in wheat background, and designated the locus as PmRc1. To develop translocation lines for facilitate the breeding use of PmRc1, we induced 1Sc chromosomal structural aberrations by irradiation and the Chinese Spring ph1b deletion mutant. Forty-three wheat-R. ciliaris 1Sc aberrations were identified and characterized by GISH/FISH and marker analysis using 1Sc diagnostic markers. The 1Sc cytological bin map was constructed and 1Sc was dissected into 28 bins. Pm resistance evaluation of the aberrations enabled us to allocate the PmRc1 into bin 1ScS-8, flanked by markers CMH93-2 and CMH114-1. Two compensative translocation lines (T1ScS•1BL and T1ScS-1AS•1AL), both carrying the PmRc1 and Pm resistant, were investigated for agronomic traits. T1ScS•1BL had increased grain size and weight, while decreased grain numbers. T1ScS-1AS•1AL had increased number of spikelet per spike. The translocation lines provide new genetic resource for improving Pm resistance in wheat breeding program.

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Development of novel wheat-rye 6RS small fragment translocation lines with powdery mildew resistance and physical mapping of the resistance gene PmW6RS

Wang,

Han,

Liu

et al. 2023

Theor Appl Genet

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Chromosomal mapping of a locus associated with adult-stage resistance to powdery mildew from Agropyron cristatum chromosome 6PL in wheat

Cited by 12 publications

References 50 publications

Introgression of Agropyron cristatum genes to counteract the negative relationship between grain number and grain weight of wheat

Introgression of Agropyron cristatum genes to counteract the negative relationship between grain number and grain weight of wheat

Cytological mapping of a powdery mildew resistance locus PmRc1 by the construction of wheat-Roegneria ciliaris structural aberration library

Development of novel wheat-rye 6RS small fragment translocation lines with powdery mildew resistance and physical mapping of the resistance gene PmW6RS

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