Chromosomal markers in three species of the genus Mytilus (Mollusca: Bivalvia)

Martı́nez-Lage, Andrés; González‐Tizón, Ana M.; Méndez, Josefina

doi:10.1038/hdy.1995.55

Cited by 30 publications

(24 citation statements)

References 30 publications

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“…To date chromosomes of five species of Mytilidae (Martí-nez- Lage et al, 1994Lage et al, , 1995Torreiro et al, 1999;Vitturi et al, 2000), one species of Donacidae (Martínez et al, 2002) and two species of Pharidae (Gonzá lez-Tizó n et al, in preparation) have been stained using CA 3 and DAPI fluorochromes. In S. marginatus the number of CA 3 bands varied from two to four; two of them are located subcentromerically in the long arms of the medium-sized subtelocentric pair 9 (Figs.…”

Section: Resultsmentioning

confidence: 99%

Cytogenetics of the razor clam Solen marginatus (Mollusca: Bivalvia: Solenidae)

Fernández‐Tajes

González‐Tizón

Martı́nez-Lage

et al. 2003

Cytogenet Genome Res

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The razor clam Solen marginatus has a diploid chromosome number of 38. The karyotype consists of one metacentric/submetacentric, three submetacentric/metacentric, five submetacentric, one submetacentric/subtelocentric, one subtelocentric/submetacentric, six subtelocentric and two telocentric chromosome pairs. Staining with chromomycin A₃ revealed bright positive bands subcentromerically in the long arms of one medium-sized subtelocentric pair, while DAPI staining showed uniform fluorescence in all chromosomes of the complement. Fluorescence in situ hybridization using an 18S-5.8S-28S rDNA probe locates these loci at the subcentromeric region of one subtelocentric pair and at the subtelomeric region of another subtelocentric pair.

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Section: Resultsmentioning

confidence: 99%

Cytogenetics of the razor clam Solen marginatus (Mollusca: Bivalvia: Solenidae)

Fernández‐Tajes

González‐Tizón

Martı́nez-Lage

et al. 2003

Cytogenet Genome Res

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“…Concerning heterochromatin DNA composition, the C-banding technique does not give any indication about the GC-richness of the underlying tandemly arrayed satellite families. Although the GC content can be partially determined using basespecific fluorochromes, this method has been performed only in a few species of bivalves, and the chromosome complements of most of them reveal scarce GC-rich regions detectable by CMA staining (CMA-bright), located at only one to three chromosomal pairs and usually coincident with NORs (Martínez-Lage et al, 1995;Martínez-Expósito et al, 1997;Torreiro et al, 1999;Hurtado and Pasantes, 2005). Therefore, the detection of GC-rich interstitial bands on eight chromosome pairs in D. trunculus by indicates an unusual genome organization among bivalves.…”

Section: Discussionmentioning

confidence: 99%

A GC-rich satellite DNA and karyology of the bivalve mollusk Donax trunculus: a dominance of GC-rich heterochromatin

Petrović

Pérez-García

Pasantes

et al. 2009

Cytogenet Genome Res

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We characterized the DTF2 satellite DNA family of the clam Donaxtrunculus and compared its chromosomal localization with cytogenetic data revealed by fluorochrome banding, C-banding, and 28S rDNA FISH. In contrast to the other satellites detected previously in this species, DTF2 is an abundant (2%) GC-rich satellite that exhibits CpG methylation. Sequence characteristics of DTF2 indicate that its evolution is not affected by constraints that might indicate some functional interactions. Fluorescence in situ hybridization revealed subtelomeric location of this satellite on a subset of 14 out of 19 D. trunculus chromosome pairs. The chromomycin A₃ (CMA) staining of GC-rich regions on D. trunculus chromosomes revealed a complex banding pattern that overlaps completely with C-bands. In total, only three bands show subtelomeric location, while 13 bands are located interstitially, one of them being coincident with the 28S rDNA hybridization signal. No bands, either CMA positive (GC-rich) or DAPI positive (AT-rich) were detected at centromeric chromosomal positions. Only two of the CMA-positive bands co-localize with the DTF2 satellite, showing a) the presence of small islands of GC-rich repetitive sequences that remained undetected by CMA/C-banding and b) the abundance of DTF2-divergent GC-rich sequences at interstitial chromosomal locations.

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“…However, only some commercial bivalves such as certain mussels, oysters, and cockles have been investigated by means of these techniques. Available information concerns all or some of the following aspects: heterochromatin distribution (Insua and ThiriotQuiévreux, 1991;Pasantes et al, 1996;Li and Havenhand, 1997), heterochromatin composition (Martínez-Lage et al, 1994Martínez-Expósito et al, 1997), location of nucleolus organizer regions (NORs) (Dixon et al, 1986;Insua andThiriot-Quiévreux, 1991, 1993;ThiriotQuiévreux and Insua, 1992;Cornet, 1993;Martínez-Expósito et al, 1994a;Insua et al, 1994, Ladrón de Guevara et al, 1994Martínez-Lage et al, 1995Thiriot-Quiévreux and Wolowicz, 1996;Li and Havenhand, 1997), G-banding pattern (Rodríguez-Romero et al, 1979;Méndez et al, 1990;Martínez-Lage et al, 1994), and replication patterns (Martínez-Expósito et al, 1994b).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Aequipecten opercularis (Bivalvia: Pectinidae) chromosomes by different staining techniques and fluorescent in situ hybridization.

1998

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The chromosomes of the queen scallop Aequipecten opercularis were studied using conventional Giemsa staining, chromosome measurements, C-banding, silver staining, and fluorescent in situ hybridization (FISH) with 18S-28S rDNA and 5S rDNA probes. The karyotype (2n = 26) consists of large metacentric (pairs 1 and 2), telocentric (pairs 3, 4, 5, 6, 7, 8, and 9), and small metacentric chromosomes (pairs 10, 11, 12, and 13). The C-bands observed can be described as major and minor C-bands which are differentiated according to the intensity of the fluorescence and the frequency of the detection. Major C-bands were found on the long arm of the chromosome pairs 6, 7, 8, and 9 in an intercalary or subterminal position. Minor C-bands were located in the centromeric region in all chromosomes of the complement and also on one arm of pairs 12 and 13 in a terminal position. Silver spots were detected on the telomere of the long arms of one or two chromosomes of pair 7 in every case, although in two individuals up to four additional silver spots were detected. These were located on pairs 8 and 9 in the same position as the C-bands. 18S-28S ribosomal genes were found by FISH on the long arm of chromosome pair 7. 5S ribosomal genes were located subterminally on one arm of metacentric pair 1, but two sites were differentiated in the case of elongated chromosomes. The results obtained allow for the identification of at least six different chromosome pairs in A. opercularis and contribute to the construction of an idiogram that is suitable for gene mapping and establishing accurate interspecific comparisons in scallops.

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Chromosomal markers in three species of the genus Mytilus (Mollusca: Bivalvia)

Cited by 30 publications

References 30 publications

Cytogenetics of the razor clam <i>Solen marginatus</i> (Mollusca: Bivalvia: Solenidae)

Cytogenetics of the razor clam <i>Solen marginatus</i> (Mollusca: Bivalvia: Solenidae)

A GC-rich satellite DNA and karyology of the bivalve mollusk <i>Donax trunculus:</i> a dominance of GC-rich heterochromatin

Characterization of Aequipecten opercularis (Bivalvia: Pectinidae) chromosomes by different staining techniques and fluorescent in situ hybridization.

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