Chromosomal transformation in the cyanobacterium Agmenellum quadruplicatum

Essich, Eberhard; Stevens, S. E.; Porter, Ronald D.

doi:10.1128/jb.172.4.1916-1922.1990

Cited by 32 publications

(13 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These are examples in which competence is induced in a certain phase of growth and in which the cells remain competent for only a relatively short period (e.g., S. pneumoniae remains competent for about 8 rain). In contrast, N. gonorrhoeae, Deinococcus radiodurans, Synechococcus and Chlorobium are competent throughout the exponential growth phase (Biswas et al 1977(Biswas et al , 1989Tirgari and Moseley 1980;Ormerod 1988;Essich et al 1990;and Wahlund and Madigan 1991). Acinetobacter calcoaceticus is a gram-negative and metabolically versatile organism (Juni 1978).…”

Section: Introductionmentioning

confidence: 99%

Physiological regulation of competence induction for natural transformation in Acinetobacter calcoaceticus

1994

View full text Add to dashboard Cite

Acinetobacter calcoaceticus induced competence for natural transformation maximally after dilution of a stationary culture into fresh medium. Competence was gradually lost during prolonged exponential growth and after entrance into the stationary state. Growth cessation and nutrient upshift were involved in the induction of competence. The level of competence of a chemostat culture of A. calcoaceticus was dependent on the nature of the growth limitation. Under potassium limitation a transformation frequency of + 1 x 10 .4 was obtained. This frequency was independent of the specific growth rate. In phosphate-, nitrogen-, and carbon-limited chemostat cultures, in contrast, the transformation frequency depended on the specific growth rate; the transformation frequency equalled _+ 10.4 at dilution rates close to g,~a• of 0.6h -1 and decreased to _+ 10 -7 at a dilution rate of 0.1 h-L We conclude that (1) DNA uptake for natural transformation in A.calcoaceticus does not serve a nutrient function and (2) competence induction is regulated via a promoter(s) that resembles the fis promoter from Escherichia coli.

show abstract

Section: Introductionmentioning

confidence: 99%

Physiological regulation of competence induction for natural transformation in Acinetobacter calcoaceticus

1994

View full text Add to dashboard Cite

show abstract

“…Synechococcus sp. strain PCC 7002 was chosen because it possesses the ability to be transformed naturally (15,42), is photoheterotrophic (32), has established plasmid transformation systems (10), and is used in genetic experiments on photosynthesis (7). These traits, the ease of growth, and the ability to extract intact genomic DNA, coupled with a small determined genome size of 3 x 106 bp (21), make this * Corresponding author.…”

mentioning

confidence: 99%

Physical genome map of the unicellular cyanobacterium Synechococcus sp. strain PCC 7002

Chen

Widger

1993

J Bacteriol

View full text Add to dashboard Cite

A physical restriction map of the genome of the cyanobacterium Synechococcus sp. strain PCC 7002 was assembled from AscI, NotI, Sall, and SfiI digests of intact genomic DNA separated on a contour-clamped homogeneous electric field pulsed-field gel electrophoresis system. An average genome size of 2.7 X 106 bp was calculated from 21 Nod, 37 Salf, or 27 SfiI frgments obtained by the digestions. The genomic map was assembled by using three different strategies: linking clone analysis, pulsed-field fragment hybridization, and individual clone hybridization to singly and doubly restriction-digested large DNA fragments. The relative positions of 21 genes or operons were determined, and these data suggest that the gene order is not highly conserved between Synechococcus sp. strain PCC 7002 and Anabaena sp. strain PCC 7120.As a model system for the study of oxygenic photosynthesis, cyanobacteria have been used as successful tools to delineate the functional proteins involved in the light and dark reactions of photosynthesis. Several strains of cyanobacteria have been used in these experiments. These include Synechocystis sp. strain PCC 6803 (28, 43, 47), Anabaena sp. strain PCC 7120 (2), Anacystis nidulans (Synechococcus sp. strain PCC 7942) (18, 36), and Synechococcus sp. strain PCC 7002 (7). Unicellular cyanobacteria have been suggested as the progenitors of higher plant chloroplasts (16), and indeed, these prokaryotes possess as a primary energy source oxygenic photosynthesis which is similar to that found in chloroplasts of higher plants. Comparison of the molecular mechanisms of the photosynthetic apparatus between higher plant chloroplasts and cyanobacteria have shown a noteworthy similarity in protein composition and function (see reference 6 for a review).In recent years, genome mapping technology has improved to the point where large physical restriction maps can be assembled by using intact genomic DNA isolated in agarose (39, 40), cut with rare restriction site enzymes, and separated on pulsed-field gel electrophoresis (PFGE) systems (12). The ability to map complex genomes rapidly allows researchers to explore complicated questions concerning global regulation of metabolism such as genome rearrangement, i.e., heterocyst formation (20), the control and rate of DNA replication termination (25), and evolutionary aspects of genome organization (31).To better understand the genetic makeup of cyanobacteria, including genes and operons and the diurnal regulation of such operons, a project was started to construct a large-scale physical map of the genome of a unicellular cyanobacterium.

show abstract

“…Only double-stranded DNA can be used for natural transformation, but it is converted into single-stranded DNA as it passes through the cell envelope. Inside the cell, the double-stranded state is restored during recombination with the chromosomal or plasmid DNA of the host (Essich et al, 1990;Barten and Lill, 1995). A calcium-dependent nuclease, located in or on the plasma membrane, was proposed to be responsible for the degradation of one of the two strands during DNA uptake in Synechocystis sp.…”

Section: Natural Transformationmentioning

confidence: 99%

“…Both Synechococcus sp. strain PCC 7002 (Stevens and Porter, 1980;Essich et al, 1990;Frigaard et al, 2004) and Synechocystis sp. strain PCC 6803 (Grigorieva and Shestakov, 1982;Barten and Lill, 1995;Heidorn et al, 2011) are naturally transformable, although the process of DNA uptake is incompletely understood.…”

Section: Natural Transformationmentioning

confidence: 99%

Biodesalination: A Case Study for Applications of Photosynthetic Bacteria in Water Treatment

et al. 2014

View full text Add to dashboard Cite

Shortage of freshwater is a serious problem in many regions worldwide, and is expected to become even more urgent over the next decades as a result of increased demand for food production and adverse effects of climate change. Vast water resources in the oceans can only be tapped into if sustainable, energy-efficient technologies for desalination are developed. Energization of desalination by sunlight through photosynthetic organisms offers a potential opportunity to exploit biological processes for this purpose. Cyanobacterial cultures in particular can generate a large biomass in brackish and seawater, thereby forming a low-salt reservoir within the saline water. The latter could be used as an ion exchanger through manipulation of transport proteins in the cell membrane. In this article, we use the example of biodesalination as a vehicle to review the availability of tools and methods for the exploitation of cyanobacteria in water biotechnology. Issues discussed relate to strain selection, environmental factors, genetic manipulation, ion transport, cell-water separation, process design, safety, and public acceptance.

show abstract

Chromosomal transformation in the cyanobacterium Agmenellum quadruplicatum

Cited by 32 publications

References 39 publications

Physiological regulation of competence induction for natural transformation in Acinetobacter calcoaceticus

Physiological regulation of competence induction for natural transformation in Acinetobacter calcoaceticus

Physical genome map of the unicellular cyanobacterium Synechococcus sp. strain PCC 7002

Biodesalination: A Case Study for Applications of Photosynthetic Bacteria in Water Treatment

Contact Info

Product

Resources

About