Chromosomally encoded ESBL genes in Escherichia coli of ST38 from Mongolian wild birds

Guenther, Sebastian; Semmler, Torsten; Stubbe, Annegret; Stubbe, Michael; Wieler, Lothar H.; Schaufler, Katharina

doi:10.1093/jac/dkx006

Cited by 70 publications

(55 citation statements)

References 20 publications

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“…coli commonly associated with urinary tract infections and bacteremia in humans (Hernandez et al ., 2013; Dolejska and Literak, 2019; Shnaiderman‐Torban et al ., 2020) but also found in wildlife (Alcala et al ., 2016; Guenther et al ., 2017). According to a recent study, the ST38/CTX‐M‐15‐resistant clone was found in a Mongolian wild bird (Guenther et al ., 2017) and the same combination was detected in a human clinical isolate in India (Guiral et al ., 2019). These results are in line with the fact that ST38 lineage is considered an expanded and pandemic clone as well as this lineage appears to be independent of antimicrobial selective pressure in clinical environments (Guenther et al ., 2017).…”

Section: Resultsmentioning

confidence: 99%

Molecular diversity of Extended‐spectrum β‐lactamase‐producing Escherichia coli from vultures in Canary Islands

Carvalho

Tejedor-Junco

González-Martín

et al. 2020

Environ Microbiol Rep

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Antimicrobial resistance among isolates from wild animals is increasingly reported. Extended‐spectrum beta‐lactamase (ESBL)‐producing Enterobacteriaceae, and particularly Escherichia coli, have spread worldwide as one of the most common multidrug‐resistant organisms. The aim of this study was to determine the carriage rate of ESBL‐producing E. coli isolates and their genetic characteristics in wild vultures from the Canary Islands. Faecal samples were collected from 22 apparently healthy free‐ranging (wild) vulture chicks from Lanzarote and Fuerteventura (Canary Islands) during July 2019. They were seeded in MacConkey agar supplemented with cefotaxime (2 μg ml−1). Colonies with typical morphology of E. coli were identified by MALDI‐TOF‐MS. Antimicrobial susceptibility was done by disk diffusion. Phenotypic detection of ESBL was performed by double‐disk tests. The presence of blaCTX‐M, blaSHV, blaTEM, blaKPC and blaOXA‐48 genes, as well as mcr‐1 (colistin resistance), tetA/tetB and int1 gene, was tested by PCR/sequencing. Phylogenetic groups and multilocus sequence typing (MLST) were determined by PCR/sequencing. ESBL‐producing E. coli isolates were detected in 5/22 tested animals (22.7%), and all isolates (one/animal) carried blaCTX‐M genes: blaCTX‐M‐15 (n = 3) and blaCTX‐M‐55 (n = 2). ESBL‐positive isolates were ascribed to phylogenetic group D (two isolates), B1 (two isolates) and A (one isolate), and five sequence types were detected (ST/phylogenetic‐group/ESBL): ST515/B1/CTX‐M‐15, ST1290/A/CTX‐M‐15, ST38/D/CTX‐M‐15, ST457/D/CTX‐M‐55 and ST6448/B1/CTX‐M‐55; this suggests a genetic diversity among these isolates. Three CTX‐M‐15‐producing isolates contained the blaTEM gene and one the tetA gene. To our knowledge, this appears to be the first report of ESBL‐producing E. coli in vulture chicks from the Canary Islands.

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Section: Resultsmentioning

confidence: 99%

Molecular diversity of Extended‐spectrum β‐lactamase‐producing Escherichia coli from vultures in Canary Islands

Carvalho

Tejedor-Junco

González-Martín

et al. 2020

Environ Microbiol Rep

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“…In this regard, ESBL‐producing Enterobacteriaceae have been isolated from a wide variety of wildlife animals worldwide (Batalha de Jesus et al., 2018; Bonnedahl et al., 2014; Dolejska & Papagiannitsis, 2018; Guenther, Ewers, & Wieler, 2011; Mohsin et al., 2017), including endangered animals for which these resistant characteristics could represent a serious setback for the species conservation programmes (Gonçalves et al., 2012). In wild birds, CTX‐M‐14‐producing E. coli has been isolated from Larus ridibundus in Sweden (Bonnedahl et al., 2010), Sylvia atricapilla in Portugal (Silva et al., 2011), Chroicocephalus brunnicephalus in Bangladesh (Hasan et al., 2014), Milvus migrans and Tyto alba in Spain (Alcalá et al., 2016), Aegypius monachus in Mongolia (Guenther et al., 2017) and Larus sp. in Alaska (Ahlstrom et al., 2019).…”

Section: Discussionmentioning

confidence: 99%

Genomic data reveal international lineages of critical priority Escherichia coli harbouring wide resistome in Andean condors (Vultur gryphus Linnaeus, 1758)

et al. 2020

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Critical priority pathogens have globally disseminated beyond clinical settings, thereby threatening wildlife. Andean Condors (Vultur gryphus) are essential for ecosystem health and functioning, but their populations are globally near threatened and declining due to anthropogenic activities. During a microbiological and genomic surveillance study of critical priority antibiotic‐resistant pathogens, we identified pandemic lineages of multidrug‐resistant extended‐spectrum β‐lactamase (ESBL)‐producing Escherichia coli colonizing Andean Condors admitted at two wildlife rehabilitation centres in South America. Genomic analysis revealed the presence of genes encoding resistance to hospital and healthcare agents among international E. coli clones belonging to sequence types (STs) ST162, ST602, ST1196 and ST1485. In this regard, the resistome included genes conferring resistance to clinically important cephalosporins (i.e., CTX‐M‐14, CTX‐M‐55 and CTX‐M‐65 ESBL genes), heavy metals (arsenic, mercury, lead, cadmium, copper, silver), pesticides (glyphosate) and domestic/hospital disinfectants, suggesting a link with anthropogenic environmental pollution. On the other hand, the presence of virulence factors, including the astA gene associated with outbreak of childhood diarrhoea and extra‐intestinal disease in animals, was identified, whereas virulent behaviour was confirmed using the Galleria mellonella infection model. E. coli ST162, ST602, ST1196 and ST1485 have been previously identified in humans and food‐producing animals worldwide, indicating that a wide resistome could contribute to rapid adaptation and dissemination of these clones at the human–animal–environment interface. Therefore, these results highlight that Andean Condors have been colonized by critical priority pathogens, becoming potential environmental reservoirs and/or vectors for dissemination of virulent and antimicrobial‐resistant bacteria and/or their genes, in associated ecosystems and wildlife.

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“…Some reports describe ESBL-Ec ST101 [28], but none report ST34 or ST195. E. coli ST38 has been extensively reported as an ESBL-Ec strain isolated both from humans and animals [29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Real-time sampling of travelers shows intestinal colonization by multidrug-resistant bacteria to be a dynamic process with multiple transient acquisitions

Kuenzli

Dunn

Dance

et al. 2019

Preprint

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Chromosomally encoded ESBL genes in Escherichia coli of ST38 from Mongolian wild birds

Cited by 70 publications

References 20 publications

Molecular diversity of Extended‐spectrum β‐lactamase‐producing Escherichia coli from vultures in Canary Islands

Molecular diversity of Extended‐spectrum β‐lactamase‐producing Escherichia coli from vultures in Canary Islands

Genomic data reveal international lineages of critical priority Escherichia coli harbouring wide resistome in Andean condors (Vultur gryphus Linnaeus, 1758)

Real-time sampling of travelers shows intestinal colonization by multidrug-resistant bacteria to be a dynamic process with multiple transient acquisitions

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