Chromosome aberrations in mouse embryos and fetuses produced by assisted reproductive technology

Abstract

The occurrence of structural chromosome aberrations in mouse one-cell embryos produced by intracytoplasmic sperm injection (ICSI) with mature spermatozoa was dependent on the type of sperm incubation medium and sperm incubation time. When cauda epididymal spermatozoa were used following incubation in bicarbonate-buffered TYH medium for 0 h (no incubation) and 0.5 h, the chromosome aberration rates (6.9% and 7.4%, respectively) in the resultant embryos were significantly higher than that (2.3%) in the IVF embryos. However, when the spermatozoa were incubated for 2−2.5 h and 6 h in the same medium, the chromosome aberration rates were reduced to the IVF embryo level (3.8% and 4.3%, respectively). When spermatozoa incubated in Hepes-buffered H-mCZB and phosphate-buffered PB1 media were used for ICSI, chromosome aberration rates in embryos were significantly high (8.6−28.1%) and increased in a time-dependent manner. On the other hand, when immature testicular spermatozoa were incubated in those three media for 0.5 h and 6 h, the incidences of resultant embryos with structural chromosome aberrations ranged between 7.4% and 11.7%, and there was no medium-and time-dependent change in these aberration rates.To evaluate transmissible risk of chromosome aberrations to offspring, two-or four-cell embryos derived from cauda epididymal spermatozoa were transferred into the oviducts of pseudopregnant females and chromosomes of live fetuses were examined on gestational day 16. One (2.0%) mosaic fetus was found when spermatozoa were incubated in TYH for 2−2.5 h, and there were four (6.7%) fetuses displaying a structurally abnormal karyotype when spermatozoa were incubated in H-mCZB for 2−2.5 h, indicating that structural chromosome aberrations generated in ICSI one-cell embryos are transmissible to offspring. The causal mechanism of structural chromosome aberrations in ICSI one-cell embryos is discussed in relation to the acrosomal plasma membrane cholesterol and the acrosome.

Preservation of chromosomal integrity in murine spermatozoa derived from gonocytes and spermatogonial stem cells surviving prenatal and postnatal exposure to γ‐rays in mice

“…The incidence of structural chromosomal aberrations in the control group was also generally higher in zygotes produced by ICSI (Table 3) than in those produced by in vitro fertilization (Table 2). This is consistent with the report that ICSI performed using testicular spermatozoa is accompanied by increased chromosome damage (Tateno, 2008).…”

Two cases of Reciprocal Chromosomal Translocation (4; 7)(p+; q−) (2; 8)(q−; q+) in Piglets Produced by ICSI

“…The incidence of chromosomal abnormalities obtained in this study using IVP of embryos by ICSI (14.28%) was higher than reported for human where approximately 5% of children born by ICSI carry a risk of chromosomal abnormalities (Bonduelle et al 1999). This result may support previous studies in mouse (Tateno 2008) and humans (Bonduelle et al 1999), but the present data seem be too small to conclude a high prevalence of chromosome anomaly in swine ICSI. Further studies must be carried out to confirm these preliminary observations.…”

“…1999). This result may support previous studies in mouse (Tateno 2008) and humans (Bonduelle et al. 1999), but the present data seem be too small to conclude a high prevalence of chromosome anomaly in swine ICSI.…”

Chromosomal integrity and DNA damage in freeze‐dried spermatozoa

“…Marchetti et al [40] suggested that mouse embryos with a small number (less than four) of paternally transmitted chromosome aberrations experienced problems in later embryonic stages. Mouse zygotes with structural chromosome aberrations generated spontaneously via ICSI can develop into live offspring carrying chromosome alterations [41,42].…”