Chromosome Aberrations Study in Human Lymphocytes from Marijuana Smokers

García, María Ángeles; Weigert, G.; Duk, Soledad; Alarcón, M.

doi:10.1007/s001289900849

Cited by 3 publications

(2 citation statements)

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“…The micronucleus (MN) assay is a genotoxicity biomonitoring method that has been used widely for evaluating risk in human populations exposed to carcinogens and/or mutagens [Tucker and Preston, 1996;García et al, 1999;Fenech, 2000]. Micronuclei are formed from acentric chromosomal fragments or whole chromosomes left behind during mitotic cellular division, allowing the detection of clastogenic and aneugenic events.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic damage in female Chilean agricultural workers exposed to mixtures of pesticides

Márquez

Villalobos

Poblete

et al. 2004

Environ. Mol. Mutagen.

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The VIII Region of Bio-Bio is a major fruit-growing area of Chile that makes intensive use of agricultural pesticides. The cytogenetic damage associated with exposure to mixtures of pesticides was evaluated by comparing peripheral blood lymphocyte micronucleus (MN) frequencies in a group of 64 female agricultural workers and 30 female controls. The exposed subjects worked during the spring and summer in thinning and pruning fruit trees and in harvesting and packing different fruits, such as raspberries, grapes, apples, and kiwis. They did not use any protective measures during their work activities. A significant increase in the frequency of binucleated cells with micronuclei (BNMN) was found in the exposed women as compared with the controls (36.94 +/- 14.47 vs. 9.93 +/- 6.17 BNMN/1000 BN cells; P < 0.001). The frequency of BNMN varied as a function of age in both the exposed and control groups, but no correlation was found between BNMN frequency and the duration of exposure. Also, smoking and other habits had no effect on MN frequency. Our study confirms that occupational exposure to pesticide mixtures results in cytogenetic damage.

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Section: Introductionmentioning

confidence: 99%

Cytogenetic damage in female Chilean agricultural workers exposed to mixtures of pesticides

Márquez

Villalobos

Poblete

et al. 2004

Environ. Mol. Mutagen.

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“…Some authors report that there is no genotoxic damage induced by marijuana use, which is the case of Jorgensen et al, (1991), who compared the frequency of SCE in lymphocytes of 22 tobacco smokers vs 22 tobacco and marijuana smokers and found no genotoxic damage [13]. On the other hand, García et al, (1999), studied 30 students exposed to marijuana and 15 not exposed, and found no differences in the percentage of chromosomal aberrations between both groups [16].…”

Section: Discussionmentioning

confidence: 96%

Clastogenic effect by recreative exposure to marijuana in individuals from the Estado de Mexico: Pilot study

Areli¹,

Fernando²,

Maricela³

et al. 2020

GSC Adv. Res. Rev.

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Cannabis sativa is the most trafficked illicit substance in the world, 147 million people consume it recreationally. This plant contains more than 100 phytocannabinoids, the most important being 1-delta-9- tetrahydrocannabinol (THC). The genotoxic effect of cannabinoids has been proven in vitro and in vivo models, where damage to nuclear DNA and chromosomal aberrations (CA) was observed. This shows the clastogenic potential of this drug. In this research, we study genotoxic damage in Mexican individuals recreationally exposed to marijuana. The study of chromosomal aberrations was performed in 48 hour lymphocyte cultures of 30 exposed and 30 no exposed individuals. 100 metaphases were analyzed per sample. The type of CA was identified and counted. The number of CAs identified in the exposed group was 344 with a median of 9.5; the no exposed group had 218 CAs, with a median of 6.5. The statistical analysis showed a significant difference between both groups, p=0.013. Chromatid and chromosomal breaks were the most frequent aberrations. Comparing the exposure time vs. frequency of CA in the exposed group, we did not find a significant difference between sub-groups from 1 to 5 and 6 to 11 years. Similarly, in the analysis of the last exposure date vs CA, no significant differences were observed between the subgroups <1 week and >1 week. Our results show an increase in the frequency of the number of CAs in the individuals recreationally exposed. These results show evidence of the genotoxic potential of marijuana.

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