Chromosome Banding Patterns of Rat Fibrosarcomas Induced by In Vitro Transformation of Embryo Cells or In Vivo Injection of Rats by 7,12-Dimethylbenz[a] anthracene

Olinici, C D; DiPaolo, Joseph A.

doi:10.1093/jnci/52.5.1627

Cited by 28 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similarity of chromosome changes between control and treated cells has also been reported for rat foetal tissue (Olinici and DiPaolo, 1974). A stem line trisomic for chromosome 6 was present in the BP-treated culture after one passage.…”

Section: Resultssupporting

confidence: 69%

Analysis of karyotype variation following carcinogen treatment of chinese hamster primary cell lines

Connell¹,

Ockey²

1977

Intl Journal of Cancer

View full text Add to dashboard Cite

Chinese hamster primary fibroblasts derived from several embryos were treated with the carcinogens benzo(a)pyrene, 7,12-dimethylbenz(a)anthracene or N-methyl-N'-nitro-N-nitrosoguanidine. Karyotype analysis, sister chromatid exchange frequency, evidence of transformation by growth in agar, cell morphology and reaction to cytocholasin B were tested at regular intervals over many culture passages. Carcinogen treatment was found to shorten the time period before onset of permanent karyotypically changed stem and side lines and in vitro transformation. Chromosomes X, 6 and 10 were more frequently involved in all cultures in these karyotype changes which were usually preceded by a period of chromosome variation. Spontaneous chromatid aberrations and aneuploidy increased in frequency with time in culture and generally appeared prior to the expression of transformation. No specific chromosomes were involved with the different carcinogens. There was no correlation between in vitro transformation and karyotype evolution and the criteria for transformation were present independently of one another. It is suggested that the lack of correlation between the parameters tested indicates that the expression of in vitro transformation is a result of selection for growth advantage from a cell population expressing an increasing degree of genetic instability and variation with time in culture.

show abstract

Section: Resultssupporting

confidence: 69%

Analysis of karyotype variation following carcinogen treatment of chinese hamster primary cell lines

Connell¹,

Ockey²

1977

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…Olinici and DiPaolo (1974) observed a number of chromosomal aberrations occurring in Syrian hamster cells treated with 7,1 2-dimethylbenz(a) anthracene, but saw similar changes in control cells.…”

Section: Discussionmentioning

confidence: 68%

“…Some workers have observed non-random chromosome change in tumours induced by 7,1 2-dimethylbenz(x)anthracene (AhlstrOm, 1974;Mitelman et al, 1972a, b), whereas Olinici and DiPaolo (1974) were unable to detect specific chromosome changes in tumours induced by the same chemical. Similarly, Benedict et al (1975) discovered-specific chromosome changes in Syrian hamster cell lines transformed by 1-/I-D-arabinofuranosylcytosine and dimethylnitrosamine, but other workers found no correlation between chromosome change and spontaneous transformation of rat cells (Jackson, Sanford and Dunn, 1970) or chemically induced transformation of Syrian hamster cells (DiPaolo, Popescu and Nelson, 1973).…”

mentioning

confidence: 99%

Chemical transformation of Chinese hamster cells: II. Appearance of marker chromosomes in transformed cells

Kirkland¹,

Venitt²

1976

Br J Cancer

View full text Add to dashboard Cite

The chromosomes of 12 samples of cultured Chinese hamster kidney and prostate cells (4 normal and 8 transformed), whose tissue culture properties have already been described (Kirkland, 1976) have been examined for numerical change and for the appearance of abnormal markers. Six transformed kidney subclones contained a consistent telocentric marker not present in the normal parental cell, and Giemsa banding demonstrated this to be the centromere and the long (q) arm of the number 4 chromosome in all cases. Two transformed prostate subclones also contained a consistent telocentric marker, not present in similarly derived normal subclones or in the normal parental cell, and Giemsa banding demonstrated this to be a different fragment (the centromere and most of the p arm) of the number 4 chromosome. It is believed that the use of a mixed-serum culture medium, designed to stabilize the karyotype of cultured Chinese hamster cells, is at least partly responsible for the detection of these transformation-associated chromosome changes. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4

show abstract

“…Causal relationship between chromosomal abnormality and tumorigenecity has been investigated by numbers of workers [2,12,16]. However, the results obtained from those previous studies were quite conflicting with one another.…”

Section: Discussionmentioning

confidence: 99%

“…However, they mentioned that modal chromosome number instability of treated cells seemed to be related to tumor-producing ability [ 16]. On the other hand, Olinici and DiPaolo have reported that in vitro malignant transformation of rat embryo cells by 7,12-dimethylbenz(a)anthracene leading to formation of fibrosarcoma in host rats were not directly caused by gross abnormalities of the chromosomes, since that neither a specific alteration of chromosome distribution nor a particular and constant chromosomal alteration was found [12]. After all, up to date, it is not clear whether these chromosomal abnormalities are directly related to the process of carcinogenesis or represent some collateral actions of chemical carcinogen.…”

Section: Discussionmentioning

confidence: 99%

Effect of 3′-Methyl-4-dimethylaminoazobenzene on liver cells from adult rat in primary culture

et al. 1982

View full text Add to dashboard Cite

Primary mass cultures of isolated liver cells, which prepared from normal adult rat by a collagenase-liver-perfusion technique, were treated with 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB) to study the production of transformed liver cells. Enzymatically isolated liver cells had high sensitivity to 3'-Me-DAB in primary culture. By 2- or 6-day treatment, mature hepatocytes remarkably decreased in their numbers due to cytotoxic effect of 3-Me-DAB, but thereafter active proliferation of epithelial-like clear cells was observed. Six-day treatment induced epithelial-like clear cells with gross chromosomal abnormalities, although 2-day treatment failed to induce transformed cells. However, the transformed epithelial-like clear cells with chromosomal abnormalities were negative for gamma-glutamyl-transpeptidase (GGT).

show abstract

Chromosome Banding Patterns of Rat Fibrosarcomas Induced by In Vitro Transformation of Embryo Cells or In Vivo Injection of Rats by 7,12-Dimethylbenz[a] anthracene

Cited by 28 publications

References 0 publications

Analysis of karyotype variation following carcinogen treatment of chinese hamster primary cell lines

Analysis of karyotype variation following carcinogen treatment of chinese hamster primary cell lines

Chemical transformation of Chinese hamster cells: II. Appearance of marker chromosomes in transformed cells

Effect of 3′-Methyl-4-dimethylaminoazobenzene on liver cells from adult rat in primary culture

Contact Info

Product

Resources

About