Chromosome Bandings and Signal Pattern of FISH Using rDNAs in <i>Bellevalia romana</i>

Abstract

SummaryThe karyotype of Bellevalia romana L., which is composed of two long metacentric chromosomes (chromosome 1), two short subtelocentric chromosomes (chromosome 2), and four short submetacentric chromosomes (chromosomes 3 and 4), were analyzed by several techniques. C-bands, CMA 3 bands, Ag-staining bands, and FISH signals of 18S rDNA appeared at the same sites on the end of an arm of chromosomes 1 and 3. A large FISH signal with 5S rDNA was localized at the end of the long arm of chromosome 2, and a small signal appeared at the interstitial region of the long arm of chromosome 1. When compared with a previous report, there were differences in the CMA 3 band on chromosome 2 and a small 5S rDNA signal on chromosome 1.

Fluorescent Chromosome Banding Patterns in Six Species of <i>Abies</i>, Pinaceae

“…After about 10 d, the primary roots growing straight were collected and treated with 0.05% colchicine for 10 h, fixed in a fixative mixed with ethanol-acetic acid-chloroform (2 : 1 : 1) and then stored in a deep freezer until use. Fluorescent banding using CMA and 4′,6-diamidino-2-phenylindole (DAPI) was adapted to plant chromosomes by Schweizer (1976) and is effective for karyotype analysis in other various plant species (Hoshi et al 2008, Urdampilleta et al 2008, Zaman and Alam 2009, Begum et al 2010, Fawzia and Alam 2011, Hoshi et al 2011, Shahla and Alam 2011, Shirakawa et al 2012, Kuroki et al 2013. The fluorescent banding procedure simplified and adapted for conifer chromosome analysis by Kondo and Hizume (1982) was used.…”

“…In most probably all genera of Pinaceae, the secondary constriction showed CMA-band and FISH signal of 45S rDNA indicating the presence of rDNA repeats (Pinus: Hizume et al , 2001Hizume et al , 2002bPicea: Hizume and Kuzukawa 1995, Brown and Carlson 1997, Shibata et al 2004Larix: Hizume et al 1988, Liu et al 2007; Pseudotsuga: Hizume andAkiyama 1992, Amarasinghe andCedrus: Dagher-Kharrat et al 2001;Abies: Puizina et al 2008) as in other plant species (Schweizer 1976, Kuroki et al 2013. In the species of Larix, Pseudotsuga and Picea, one CMAband is consistent with the FISH signal of 5S rDNA (Hizume et al 1996, Amarasinghe and Carlson 1998, Liu et al 2007.…”

FISH with Padlock Probes Can Efficiently Reveal the Genomic Position of Low or Single-Copy DNA Sequences

“…Fluorescence in situ hybridization (FISH) can analyze the genomic position and distribution of DNA sequences in nuclei and on chromosomes (Ebadi-Almas et al 2012, Shirakawa et al 2012, Ikeda et al 2013, Kuroki et al 2013, Ruan et al 2013, Yokomi et al 2013, Suto et al 2013, Lombello et al 2014, Abozeid et al 2015, Kantek et al 2015, Matsunaga 2016, Monkheang et al 2016, Shibata et al 2016, Tantivit et al 2016, Jantarat et al 2017, Taguchi et al 2017. Labeling of DNA probes for FISH has been generally performed through enzymatic incorporation of labeled dNTPs by PCR or nick translation (Shibata and Hizume 2015).…”

Chromatin Tagging Systems Contribute to Live Imaging Analyses for Chromatin Dynamics

“…Fluorescent in situ hybridization (FISH) has revealed chromosomal loci or regions of DNA sequences on mitotic chromosomes (Suto et al 2012, Ikeda et al 2013, Kuroki et al 2013, Ruan et al 2013, Yokomi et al 2013, Suto et al 2013, Kantek et al 2015, Shibata and Hizume 2015. Three dimensional (3D) FISH can visualize the location of DNA sequences in interphase nuclei and show the alteration of chromatin structure (Matsunaga 2016).…”