Chromosome Condensation in
            <i>Xenopus</i>
            Mitotic Extracts Without Histone H1

Ohsumi, Keita; Katagiri, Chiaki; Kishimoto, Takeo

doi:10.1126/science.8266099

Cited by 154 publications

(104 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been much easier to establish some of the things that these H1 variants do not do during early embryonic development. Depletion of B4 from Xenopus oocyte extracts, for instance, has demonstrated that the presence of this histone is not required for the assembly of morphologically normal pronuclei which are capable of DNA replication (Ohsumi et al, 1993;Dasso et al, 1994). Similar experiments also established that B4 did not contribute to the physiological spacing of nucleosomes .…”

Section: Fertilizationsupporting

confidence: 51%

Dynamic alterations of linker histone variants during development

Godde¹,

Ura²

2009

Int. J. Dev. Biol.

View full text Add to dashboard Cite

The process of development can be viewed as a series of linker histone replacements which take place throughout spermatogenesis and oogenesis, as well as following fertilization or somatic nuclear transfer (SNT). Although few of the histone H1 variants in question have been shown to be essential for viability, the timing of their appearance as well as the affinity with which they are able to bind to chromatin seem to be important factors in their developmental role. A looser binding of linker histones to chromatin seems to correlate with the meiotic phases of gametogenesis and the establishment of a totipotent, as well as the maintenance of a pluripotent, state in early embryos, while tighter binding of linker histones to chromatin appears to be associated with the mitotic phases, as well as the increased levels of condensation that are required for the packaging of DNA into sperm. This latter process also involves the binding of certain basic non-histone proteins to DNA. While all proteins involved in chromatin compaction during development are highly basic in nature, in general they can be seen to change from lysinerich variants to arginine-rich ones, and back again. The fact that linker histone transitions are conserved across diverse metazoan species speaks of their importance in packaging DNA in a variety of ways during this crucial period.

show abstract

Section: Fertilizationsupporting

confidence: 51%

Dynamic alterations of linker histone variants during development

Godde¹,

Ura²

2009

Int. J. Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…As shown in previous studies (23,34), extensive nuclease digestion of B4-free nucleosomes, i.e., nucleosome cores, produced a DNA fragment of 146 bp (Fig. 2H, lane 2), which was protected by the core histones alone, whereas nuclease digestion of nucleosomes containing B4 produced a DNA fragment of Ϸ170 bp (Fig.…”

Section: Involvement Of Nap-1 In Nucleosome Assembly Of Sperm Chromatmentioning

confidence: 97%

“…Demembranated Xenopus sperm prepared as described (23) was incubated with egg extract and histidine-tagged p60 xNAP-1 (His-xNAP-1) solutions at a final concentration of 5,000͞ l. After 30-min incubation, sperm chromatin was sedimented onto a sucrose cushion by centrifugation and examined for linker histone binding. SDS͞PAGE of acidextracted proteins and micrococcal nuclease digestion assay were performed as described (23).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Nucleosome assembly protein-1 is a linker histone chaperone in Xenopus eggs

Shintomi

Iwabuchi²,

Saeki³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

In eukaryotic cells, genomic DNA is primarily packaged into nucleosomes through sequential ordered binding of the core and linker histone proteins. The acidic proteins termed histone chaperones are known to bind to core histones to neutralize their positive charges, thereby facilitating their proper deposition onto DNA to assemble the core of nucleosomes. For linker histones, however, little has been known about the regulatory mechanism for deposition of linker histones onto the linker DNA. Here we report that, in Xenopus eggs, the linker histone is associated with the Xenopus homologue of nucleosome assembly protein-1 (NAP-1), which is known to be a chaperone for the core histones H2A and Xenopus laevis ͉ chromatosome assembly ͉ cell-free system

show abstract

“…Phosphorylation of histone H1 during entrance of cell to mitosis was reported over three decades ago (5), and the role of this modification in chromatin condensation has been postulated (6). More recent studies, however, have shown that histone H1 is not required for chromatin to undergo mitotic condensation (7,8).…”

mentioning

confidence: 99%

Sequential phosphorylation of Ser‐10 on histone H3 and ser‐139 on histone H2AX and ATM activation during premature chromosome condensation: Relationship to cell‐cycle phase and apoptosis

et al. 2006

View full text Add to dashboard Cite

Background: Histone H1 and H3 phosphorylation associated with chromatin condensation during mitosis has been studied extensively. Less is known on histone modifications that occur during premature chromosome condensation (PCC). The aim of the present study was to reveal the status of histone H3 and H2AX phosphorylation on Ser-10 and Ser-139, respectively, as well as ATM activation through phosphorylation on Ser-1981, during PCC, and relate these events to cell-cycle phase and to initiation of apoptosis. Materials and Methods: To induce PCC, A549 and HL-60 cells were exposed to the phosphatase inhibitor calyculin A (Cal A). Phosphorylation of histone H3 and H2AX as well as ATM activation were detected immunocytochemically concurrent with analysis of cellular DNA content and activation of caspase-3, a marker of apoptosis. The intensity of cellular fluorescence was measured by flow-or laser scanning cytometry. Results: Induction of PCC led to rapid histone H3 phosphorylation, followed by activation of ATM and then H2AX

show abstract

Chromosome Condensation in Xenopus Mitotic Extracts Without Histone H1

Cited by 154 publications

References 36 publications

Dynamic alterations of linker histone variants during development

Dynamic alterations of linker histone variants during development

Nucleosome assembly protein-1 is a linker histone chaperone in Xenopus eggs

Sequential phosphorylation of Ser‐10 on histone H3 and ser‐139 on histone H2AX and ATM activation during premature chromosome condensation: Relationship to cell‐cycle phase and apoptosis

Contact Info

Product

Resources

About