Chromosome-condensed G1 phase yeast cells are tolerant to desiccation stress

Zhang, Zhaojie; Zhang, Gracie R.

doi:10.15698/mic2022.02.770

Cited by 4 publications

(5 citation statements)

References 46 publications

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“…They all have condensed chromosomes. The condensed chromosome suggests that genes are more silenced, which is confirmed by the low metabolic activities of these cells [24] (Figure 1). One possibility is this could be a feature developed through evolution, in which a small portion of cells is always prepared to encounter the unpredicted and sudden changes of their environment.…”

Section: No Pain No Gain-low Stress Produces More Tolerancementioning

confidence: 65%

“…One possibility is this could be a feature developed through evolution, in which a small portion of cells is always prepared to encounter the unpredicted and sudden changes of their environment. [24]).…”

Section: No Pain No Gain-low Stress Produces More Tolerancementioning

confidence: 99%

“…In budding yeast, elevated levels of trehalose are found only in stationary cells, which are desiccation tolerant. The trehalose concentration is very low in exponentially growing cells, and these cells are very sensitive to desiccation [22,24]. Stationary yeast cells have a metabolic mode distinct from the exponentially growing cells.…”

Section: Physiological Changesmentioning

confidence: 99%

“…Blue: Fluorescence brightener 28 staining of cell wall and bud scars, which indicate the replicative age of yeast cells. The desiccation resistant cells (D r ) are in G1 but have higher fluorescence intensity (Redraw based on[24]).…”

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confidence: 99%

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How to Survive without Water? A Short Lesson from Desiccation Tolerance of the Budding Yeast

Robison,

Ren,

Zhang

2024

Preprint

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Water is essential for all life on earth. It is a major component that makes up living organisms and plays a vital role in multiple biological processes. It provides a medium for chemical and enzymatic reactions in the cell and is a major player for osmoregulation and maintenance of cell turgidity. Despite this, many organisms, called anhydrobiotes, are capable of surviving under extremely dehydrated conditions. Less is known about how anhydrobiotes adapt and survive under the desiccation stress. Studies have shown that morphological and physiological changes occur in anhydrobiotes in response to desiccation stress. Certain disaccharides and proteins, including heat shock proteins, intrinsically disordered proteins, and hydrophilins, play important roles in the desiccation tolerance of the anhydrobiotes. In this review, we summarize the recent findings of the desiccation tolerance in the budding yeasts Saccharomyces cerevisiae. We also propose that the yeast under desiccation could be used as a model to study neurodegenerative disorders.

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Section: No Pain No Gain-low Stress Produces More Tolerancementioning

confidence: 65%

Section: No Pain No Gain-low Stress Produces More Tolerancementioning

confidence: 99%

Section: Physiological Changesmentioning

confidence: 99%

mentioning

confidence: 99%

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How to Survive without Water? A Short Lesson from Desiccation Tolerance of the Budding Yeast

Robison,

Ren,

Zhang

2024

Preprint

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“…Herein, this study compares the proteomes and metabolomes of WT C. albicans (strain DK318) when grown in solid (agar plates) versus liquid (broth) media, with a specific focus on the changes occurring in solid media (agar plates). Although debatable, the surrounding conditions at the stationary phase are believed to be more like those observed in solid media [56,57], such as nutrient limitations, individual competition, accumulation of byproducts, and, eventually, a steady growth rate. As proof of concept, we selected a single time point 16 h after inoculation for both the solid and liquid stationary phase.…”

Section: Resultsmentioning

confidence: 99%

Multi-Omics Profiling of Candida albicans Grown on Solid Versus Liquid Media

Alhameed,

Semreen,

Hamad

et al. 2023

Microorganisms

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Candida albicans is a common pathogenic fungus that presents a challenge to healthcare facilities. It can switch between a yeast cell form that diffuses through the bloodstream to colonize internal organs and a filamentous form that penetrates host mucosa. Understanding the pathogen’s strategies for environmental adaptation and, ultimately, survival, is crucial. As a complementary study, herein, a multi-omics analysis was performed using high-resolution timsTOF MS to compare the proteomes and metabolomes of Wild Type (WT) Candida albicans (strain DK318) grown on agar plates versus liquid media. Proteomic analysis revealed a total of 1793 proteins and 15,013 peptides. Out of the 1403 identified proteins, 313 proteins were significantly differentially abundant with a p-value < 0.05. Of these, 156 and 157 proteins were significantly increased in liquid and solid media, respectively. Metabolomics analysis identified 192 metabolites in total. The majority (42/48) of the significantly altered metabolites (p-value 0.05 FDR, FC 1.5), mainly amino acids, were significantly higher in solid media, while only 2 metabolites were significantly higher in liquid media. The combined multi-omics analysis provides insight into adaptative morphological changes supporting Candida albicans’ life cycle and identifies crucial virulence factors during biofilm formation and bloodstream infection.

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A Systematic Review on Quiescent State Research Approaches in S. cerevisiae

Opalek

Tutaj

Pirog

et al. 2023

Cells

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Quiescence, the temporary and reversible arrest of cell growth, is a fundamental biological process. However, the lack of standardization in terms of reporting the experimental details of quiescent cells and populations can cause confusion and hinder knowledge transfer. We employ the systematic review methodology to comprehensively analyze the diversity of approaches used to study the quiescent state, focusing on all published research addressing the budding yeast Saccharomyces cerevisiae. We group research articles into those that consider all cells comprising the stationary-phase (SP) population as quiescent and those that recognize heterogeneity within the SP by distinguishing phenotypically distinct subpopulations. Furthermore, we investigate the chronological age of the quiescent populations under study and the methods used to induce the quiescent state, such as gradual starvation or abrupt environmental change. We also assess whether the strains used in research are prototrophic or auxotrophic. By combining the above features, we identify 48 possible experimental setups that can be used to study quiescence, which can be misleading when drawing general conclusions. We therefore summarize our review by proposing guidelines and recommendations pertaining to the information included in research articles. We believe that more rigorous reporting on the features of quiescent populations will facilitate knowledge transfer within and between disciplines, thereby stimulating valuable scientific discussion.

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Chromosome-condensed G1 phase yeast cells are tolerant to desiccation stress

Cited by 4 publications

References 46 publications

How to Survive without Water? A Short Lesson from Desiccation Tolerance of the Budding Yeast

How to Survive without Water? A Short Lesson from Desiccation Tolerance of the Budding Yeast

Multi-Omics Profiling of Candida albicans Grown on Solid Versus Liquid Media

A Systematic Review on Quiescent State Research Approaches in S. cerevisiae

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