Chromosome control of apomixis in maize-gamagrass hybrids

Belova, Irina V.; Tarakanova, T. K.; Abdyrahmanova, E. A.; Sokolov, V. A.; Панихин, П. А.

doi:10.1134/s1022795410090103

Cited by 8 publications

(7 citation statements)

References 4 publications

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“…It is a warm-season C4 facultative apomictic perennial forage grass having a remarkable ability to withstand various biotic and abiotic stresses (Eubanks 2006). Artificial crosses are possible between corn and T. dactyloides with more or less success but generally involve embryo rescue (Leblanc et al 2009;Belova et al 2010). Although F1 hybrids between maize and T. dactyloides showed an abnormal meiosis, pairing between chromosomes from both parental species indicated the possibility of genetic recombination between them (Molina et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Allopolyploidization facilitates gene flow and speciation among corn, Zea perennis and Tripsacum dactyloides

Iqbal

Cheng

et al. 2019

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Main conclusion Tripsacum dactyloides is closely related to Zea mays since Zea perennis and the MTP tri-species hybrid have four possible reproductive modes. Eastern gamagrass (Tripsacum dactyloides L.) and tetraploid perennial teosinte (Zea perennis) are well known to possess genes conferring resistance against biotic and abiotic stresses as well as adaptation to flood and aluminum toxic soils. However, plant breeders have been hampered to utilize these and other beneficial traits for maize improvement due to sterility in their hybrids. By crossing a tetraploid maize-inbred line × T. dactyloides, a female fertile hybrid was produced that was crossed with Z. perennis to yield a tri-genomic female fertile hybrid, which was backcrossed with diploid maize to produce BC 1 and BC 2. The tri-genomic hybrid provided a new way to transfer genetic material from both species into maize by utilizing conventional plant breeding methods. On the basis of cytogenetic observations using multi-color genomic in situ hybridization, the progenies were classified into four groups, in which chromosomes could be scaled both up and down with ease to produce material for varying breeding and genetic purposes via apomixis or sexual reproduction. In the present study, pathways were found to recover maize and to obtain specific translocations as well as a speedy recovery of the T. dactyloides-maize addition line in a second backcross generation. However, phenotypes of the recovered maize were in most cases far from maize as a result of genetic load from T. dactyloides and Z. perennis, and could not be directly used as a maize-inbred line but could serve as an intermediate material for maize improvement. A series of hybrids was produced (having varying chromosome number, constitution, and translocations) with agronomic traits from all three parental species. The present study provides an application of overcoming the initial interspecific barriers among these species. Moreover, T. dactyloides is closely related to Z. mays L. ssp. mays since Z. perennis and the MTP tri-species hybrid have four possible reproductive modes. Keywords Apomixes • Chromosome translocation • Eastern gamagrass • Germplasm expansion • Perennial tetraploid teosinte • Tri-genomic bridge plant Abbreviations mcGISH Multi-color Genomic in Situ-Hybridization MTP Maize, T. dactyloides, and Z. perennis hybrid Mz Maize, Zea mays L. ssp. mays Zp Zea perennis

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Section: Introductionmentioning

confidence: 99%

Allopolyploidization facilitates gene flow and speciation among corn, Zea perennis and Tripsacum dactyloides

Iqbal

Cheng

et al. 2019

Planta

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“…Traditionally, the technique used to create apomictic accessions is introgressing apomixis into sexual species from apomictic wild relatives. In maize, the tetraploid species Tripsacum dactyloides L. was used as the donor of apomixis [17][18][19]. Apomictic hybrids obtained in these crosses contained the Tripsacum chromosomes that negatively impacted agronomically important traits tightly linked to the trait.…”

Section: Introductionmentioning

confidence: 99%

Pseudogamous Apomixis in Maize and Sorghum in Diploid-Tetraploid Crosses

Tsvetova¹,

Lev²,

Italianskaya³

2019

Phyton

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Apomictic seed development is a complex process including formation of unreduced embryo sac, parthenogenetic embryo development from the egg cell, and endosperm formation either autonomously, or due to fertilization of polar nuclei by the sperm (under pseudogamous form of apomixis). In the latter case, an obstacle to the normal endosperm development is disturbance of maternal (m) -to-paternal (p) genomic ratio 2m: 1p that occurs in the cases of pollination of unreduced embryo sac with haploid sperms. Usage of tetraploid pollinators can overcome this problem because in such crosses maternal-to-paternal genomic ratio is 4m: 2p that provides formation of kernels with plump endosperm. Using tetraploid lines as pollen parents we observed formation of plump kernels on the ears and panicles of diploid maize and sorghum accessions. These kernels had hybrid endosperm and diploid maternaltype embryo or hybrid embryo with different ploidy level (2n, 3n, 4n). The frequencies of plump kernels on the ear ranged from 0.2-0.3% to 5.7-6.2% counting from the number of ovaries. Maternal-type plants were found in two maize lines, their frequency varying from 10.7 to 37.5% of the progeny plants. In CMS-lines of sorghum pollinated with tetraploid sorghum accessions, the frequency of plump kernels ranged from 0.6 to 14.0% counting from the number of ovaries; the frequency of maternal-type plants varied from 33.0 up to 96.1%. The hybrid nature of endosperm of the kernels that gave rise to maternal-type plants has been proved by marker gene expression and by SDS-electrophoresis of endosperm proteins. These data testify to variable modes of seed formation under diploid × tetraploid crosses in maize and sorghum both by amphi-and by apomixis. Therefore, usage of tetraploid pollinators might be a promising approach for isolation of apomixis in maize and sorghum accessions.

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“…The hybrids reported in literature were generally accomplished with maize used as the female parent (Mangelsdorf and Reeves 1931; James 1979; Bernard and Jewell 1985; Leblanc et al 1995; Duvick et al 2006; Belova et al 2010). When assessing the environmental risk of gene flow from transgenic maize to eastern gamagrass, the crosses where eastern gamagrass is used as a female parent would be most informative.…”

Section: Introductionmentioning

confidence: 99%

“…Because Tripsacum and Zea are closely related genomes, eastern gamagrass has been considered as a possible source of favorable and diverse genes for maize improvement (Newell and de Wet 1974 ; de Wet et al 1982 ; Li et al 1999 ; de Wet and Harlan 1974 ; Leblanc et al 1995 ; Reeves and Bockholt 1964 ; Duvick et al 2006 ; Hajjar and Hodgkin 2007 ). Tripsacum ’s wide tolerance to soil conditions, resistance to some common maize diseases and insect pests, favorable fatty acid composition and ability to reproduce through apomixis are examples of characteristics that make this species potentially valuable for maize breeding programs (Doebley 1983 ; Bernard and Jewell 1985 ; Leblanc et al 1995 ; Gurney et al 2003 ; Duvick et al 2006 ; Belova et al 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Assessment of the potential for gene flow from transgenic maize (Zea mays L.) to eastern gamagrass (Tripsacum dactyloides L.)

et al. 2017

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Eastern gamagrass (Tripsacum dactyloides L.) belongs to the same tribe of the Poaceae family as maize (Zea mays L.) and grows naturally in the same region where maize is commercially produced in the USA. Although no evidence exists of gene flow from maize to eastern gamagrass in nature, experimental crosses between the two species were produced using specific techniques. As part of environmental risk assessment, the possibility of transgene flow from maize to eastern gamagrass populations in nature was evaluated with the objectives: (1) to assess the seeds of eastern gamagrass populations naturally growing near commercial maize fields for the presence of a transgenic glyphosate-tolerance gene (cp4 epsps) that would indicate cross-pollination between the two species, and (2) to evaluate the possibility of interspecific hybridization between transgenic maize used as male parent and eastern gamagrass used as female parent. A total of 46,643 seeds from 54 eastern gamagrass populations collected in proximity of maize fields in Illinois, USA were planted in a field in 2014 and 2015. Emerged seedlings were treated with glyphosate herbicide and assessed for survival. An additional 48,000 seeds from the same 54 eastern gamagrass populations were tested for the presence of the cp4 epsps transgene markers using TaqMan® PCR method. The results from these trials showed that no seedlings survived the herbicide treatment and no seed indicated presence of the herbicide tolerant cp4 epsps transgene, even though these eastern gamagrass populations were exposed to glyphosate-tolerant maize pollen for years. Furthermore, no interspecific hybrid seeds were produced from 135 hand-pollination attempts involving 1529 eastern gamagrass spikelets exposed to maize pollen. Together, these results indicate that there is no evidence of gene flow from maize to eastern gamagrass in natural habitats. The outcome of this study should be taken in consideration when assessing for environmental risks regarding the consequence of gene flow from transgenic maize to its wild relatives.Electronic supplementary materialThe online version of this article (doi:10.1007/s11248-017-0020-7) contains supplementary material, which is available to authorized users.

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Chromosome control of apomixis in maize-gamagrass hybrids

Cited by 8 publications

References 4 publications

Allopolyploidization facilitates gene flow and speciation among corn, Zea perennis and Tripsacum dactyloides

Allopolyploidization facilitates gene flow and speciation among corn, Zea perennis and Tripsacum dactyloides

Pseudogamous Apomixis in Maize and Sorghum in Diploid-Tetraploid Crosses

Assessment of the potential for gene flow from transgenic maize (Zea mays L.) to eastern gamagrass (Tripsacum dactyloides L.)

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