Chromosome ideograms of the laboratory rat <i>(Rattus norvegicus)</i> based on high-resolution banding, and anchoring of the cytogenetic map to the DNA sequence by FISH in sample chromosomes

Abstract

A detailed banded ideogram representation of the rat chromosomes was constructed based on actual G-banded prometaphase chromosomes. The approach yielded 535 individual bands, a significant increase compared to previously presented ideograms. The new ideogram was adapted to the existing band nomenclature. The gene locus positions in the rat draft DNA sequence were compared to the chromosomal positions as determined by dual-color FISH, using rat (RNO) chromosomes 6 and 15 and a segment of RNO4 as sample regions. It was found that there was generally an excellent correlation in the chromosome regions tested between the relative gene position in the DNA molecules and the sub-chromosomal localization by FISH and subsequent information transfer on ideograms from measurements of chromosomal images. However, in the metacentric chromosome (RNO15), the correlation was much better in the short arm than in the long arm, suggesting that the centromeric region may distort the linear relationship between the chromosomal image and the corresponding DNA molecule.

Defining the Sister Rat Mammary Tumor Cell Lines HH-16 cl.2/1 and HH-16.cl.4 as an In Vitro Cell Model for Erbb2

“…RNO6 painting and the rat PAC clone RP31- 202O5 were used to access the amplification status of Mycn . RP31-202O5 was earlier confirmed to contain Mycn gene and mapped to RNO 6q15.3-16 [23] and was also used in this work for the accurate identification of HH-16 cl.2/1 breakpoint regions. During HH-16 cl.2/1 cytogenetic characterization, it was possible to verify that this gene was present in three copies distributed among two intact RNO6 chromosomes and in the derivative chromosomes der(19)t(6;19)(q14;q12), der(18;19)t(18;19)(p10,q10)t(6;19)(q14;q12),der(19)t(6;19)(q14;q12) and der(19)t(4;19)(q31;p11)t(6;19)(q14;q12), indicating that a partial trisomy of RNO6 was involved in a translocation.…”

“…The selected clones contained regions of rat chromosomes 6 (RP31-262B4, CH230-208E5, RP31- 202O5, RP31-039D3, CH230-10B5) and 15 (CH230-174M18, CH230-9A5, CH230-215E5, CH230-27O13, CH230-165C24, CH230-117H20), which were physically mapped in a previous study [23]. The BAC/PAC results allowed the identification of the breakpoint regions of the derivative chromosomes involving RNO6 to der(19)t(6;19)(q14;q12), der(19)t(4;19)(q31;p11)t(6;19)(q14;q12) and der(18;19)t(18;19)(p10,q10)t(6;19)(q14;q12), assigning the location of the breakpoint in all of these chromosomes to band 6q14, above the region included within clone RP31-262B4 (Figure 3).…”

SKY analysis revealed recurrent numerical and structural chromosome changes in BDII rat endometrial carcinomas

“…The majority of tumors displayed a complex pattern of numerical and structural aberrations (Figure 1). Using the International System for Human Cytogenetic Nomenclature (ISCN 1995) and literature on nomenclature for G-bands in rat chromosome [25,26], we determined the most common cytogenetic changers among the tumors (Table 2). …”

Karyological Studies ofBiomphalaria tenagophila (d'Orbigny, 1835) (Gastropoda: Planobidae) from Rio de Janeiro, Brazil

“…The study of an organism genome at the chromosomal level can be used in differentiating one species from another, i.e., the analysis of chromosome numbers, size, and centromere positions [11,12,13]. In fact, modern cytogenetic techniques have since mid-1990s been adopted for studies of Gastropoda.…”