Chromosome-level genome assembly and annotation of two lineages of the ant Cataglyphis hispanica: stepping stones towards genomic studies of hybridogenesis and thermal adaptation in desert ants

Darras, Hugo; Araujo, Natália de Souza; Baudry, Lyam; Guiglielmoni, Nadège; Lorite, Pedro; Marbouty, Martial; Rodríguez, Fernando; Arkhipova, Irina R.; Koszul, Romain; Flot, Jean-François; Aron, Serge

doi:10.24072/pcjournal.140

Cited by 5 publications

(5 citation statements)

References 79 publications

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“…For the two Cataglyphis species and for Formica fusca , we generated both, the full de novo and the reference‐guided assemblies. The reference‐guided approach for the Cataglyphis was based on the genome of Cataglyphis hispanica (assembly ASM419527v1; Darras et al, 2022 ) and that of F . fusca in the genome of Formica exsecta (assembly ASM365146v1; Dhaygude et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

“…Species-specific orthogroups accounted for 9178 (26.9%) of all orthogroups and contained 29,645 genes (10.1% of the total), while 8753 orthogroups contained all species and only 321 were single copies (Table 4). Compared with genomic orthology estimations (Darras et al, 2022), the transcriptomic-based orthology analyses resulted in more species-specific and fewer single-copy orthogroups, which can be expected since transcriptomic data might include multiple isoforms per gene (see BUSCO duplication ratio in Table 2). For this reason, we estimated the relevance of new genes not only based on their frequency but comparatively, by contrasting the relative proportion of transcripts in each orthogroup category between the transcriptome and among the DET.…”

Section: Greater Gene Expression Changes Are Associated With Increase...mentioning

confidence: 99%

“…The reference-guided approach for the Cataglyphis was based on the genome of Cataglyphis hispanica (assembly ASM419527v1; Darras et al, 2022) and that of F. fusca in the genome of Formica exsecta (assembly ASM365146v1; Dhaygude et al, 2019). These two independent assemblies were then concatenated.…”

Section: Transcriptome Assembly and Differential Expressionmentioning

confidence: 99%

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Facing lethal temperatures: Heat‐shock response in desert and temperate ants

Araujo,

Perez,

Willot

et al. 2023

Ecology and Evolution

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Global climate changes may cause profound effects on species adaptation, particularly in ectotherms for whom even moderate warmer temperatures can lead to disproportionate heat failure. Still, several organisms evolved to endure high desert temperatures. Here, we describe the thermal tolerance survival and the transcriptomic heat stress response of three genera of desert (Cataglyphis, Melophorus, and Ocymyrmex) and two of temperate ants (Formica and Myrmica) and explore convergent and specific adaptations. We found heat stress led to either a reactive or a constitutive response in desert ants: Cataglyphis holgerseni and Melophorus bagoti differentially regulated very few transcripts in response to heat (0.12% and 0.14%, respectively), while Cataglyphis bombycina and Ocymyrmex robustior responded with greater expression alterations (respectively affecting 0.6% and 1.53% of their transcriptomes). These two responsive mechanisms—reactive and constitutive—were related to individual thermal tolerance survival and convergently evolved in distinct desert ant genera. Moreover, in comparison with desert species, the two temperate ants differentially expressed thousands of transcripts more in response to heat stress (affecting 8% and 12.71% of F. fusca and Myr. sabuleti transcriptomes). In summary, we show that heat adaptation in thermophilic ants involved changes in the expression response. Overall, desert ants show reduced transcriptional alterations even when under high thermal stress, and their expression response may be either constitutive or reactive to temperature increase.

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Section: Methodsmentioning

confidence: 99%

Section: Greater Gene Expression Changes Are Associated With Increase...mentioning

confidence: 99%

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Facing lethal temperatures: Heat‐shock response in desert and temperate ants

Araujo,

Perez,

Willot

et al. 2023

Ecology and Evolution

Self Cite

View full text Add to dashboard Cite

show abstract

“…For the two Cataglyphis species and for Formica fusca we generated both, the full de novo and the reference guided assemblies. The reference guided approach for the Cataglyphis was based on the genome of Cataglyphis hispanica (assembly ASM419527v1 - Darras et al, 2022) and that of F. fusca in the genome of Formica exsecta (assembly ASM365146v1 - Dhaygude et al, 2019). These two independent assemblies were then concatenated.…”

Section: Methodsmentioning

confidence: 99%

“…Species-specific orthogroups accounted for 9,178 (26.9%) of all orthogroups and contained 29,645 genes (10.1% of the total), while 8,753 orthogroups contained all species and only 321 were single copy (Table S5). Compared to genomic orthology estimations (Darras et al, 2022), the transcriptomic based orthology analyses resulted in more species-specific and less single copy orthogroups which can be expected since transcriptomic data include multiple isoforms per gene (see BUSCO duplication ratio at Table S2). For this reason, we estimated the relevance of new genes not only based on their frequency but comparatively, by contrasting the relative proportion of transcripts in each orthogroup category between the transcriptome and among the DET.…”

Section: Molecular Pathways Responsive To Heat Stress In Desert Ants ...mentioning

confidence: 99%

Facing lethal temperatures: heat shock response in desert and temperate ants

Araujo

Perez

Willot

et al. 2022

Preprint

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Several genera of desert ants have adapted to endure prolonged exposure to high temperatures. The study of these ants is thus essential to unravel how species respond and adapt to thermal stress. We investigated the thermal tolerance and the transcriptomic heat stress response of three desert ant genera adapted to withstand high temperatures (Cataglyphis, Melophorus and Ocymyrmex) and two temperate genera (Formica and Myrmica) to explore convergent and specific adaptations. We found a variable transcriptomic response among desert species exposed to similar levels of physiological heat-stress: Cataglyphis holgerseni and Melophorus bagoti differentially regulated very few transcripts (54 and 53, respectively), while Cataglyphis bombycina and Ocymyrmex robustior showed greater expression alterations (affecting 253 and 698 transcripts, respectively). These two responsive mechanisms of lower and higher gene expression changes were related to desert species thermal tolerance survival pattern and convergently evolved in distinct desert ant genera. Moreover, the two temperate species tested differentially expressed thousands of genes more than desert ants in response to heat stress, suggesting that keeping restrained gene expression is an important adaptation in heat adapted species. Finally, we found a significant overlap of the molecular pathways activated in response to heat-stress in temperate and desert species. This indicates that the molecular processes involved in heat-stress survival appears to be evolutionary conserved in ants, even though taxonomically restricted genes also participated in the heat stress response.

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Multisensory navigation and neuronal plasticity in desert ants

Rößler¹

2023

Trends in Neurosciences

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Chromosome-level genome assembly and annotation of two lineages of the ant Cataglyphis hispanica: stepping stones towards genomic studies of hybridogenesis and thermal adaptation in desert ants

Abstract: Aron (2022) Chromosome-level genome assembly and annotation of two lineages of the ant Cataglyphis hispanica: stepping stones towards genomic studies of hybridogenesis and thermal adaptation in desert ants, Peer Community Journal, 2: XX.

Cited by 5 publications

References 79 publications

Facing lethal temperatures: Heat‐shock response in desert and temperate ants

Facing lethal temperatures: Heat‐shock response in desert and temperate ants

Facing lethal temperatures: heat shock response in desert and temperate ants

Multisensory navigation and neuronal plasticity in desert ants

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