Chromosome localization of the genes for ENO1, HK1, ADK, ACP2, MPI, ITPA, ACON1 and ?-GAL in the American mink (Mustela vison)

Abstract

Twenty-eight American mink × Chinese hamster somatic cell hybrids were analysed for the expression of mink enzymes and the segregation of mink chromosomes. The results demonstrated that the gene for enolase-1 is located on the long arm of mink chromosome 2, and those for hexokinase-1 and adenosine kinase, on its short arm. Segregation analysis of mink chromosomes and mink acid phosphatase-2, mannose phosphate isomerase, inosine triphosphatase and aconitase-1 provided data allowing us to assign the genes for these markers to mink chromosomes 7, 10, 11 and 12, respectively. The expression of mink α-galactosidase was highly coincidental with mink × chromosome as well as with its markers: hypoxanthine-phosphoribosyltransferase, glucose-6-phosphate dehydrogenase and phosphoglycerate kinase-1. This result confirms the assignment of the gene for α-galactosidase to the mink × chromosome.

Mapping of the silver fox genes: assignments of the genes for ME1, ADK, PP, PEPA, GSR, MPI, and GOT1

“…PEPA. and ADK were visualized as described elsewhere (Rubtsov et al, 1981(Rubtsov et al, , 1982Gradov et al, 1983) and those of MPI and GSR using the standard techniques o f Harris and Hopkinson (1976).…”

Chromosomal and regional localization of the genes for UMPH2, APRT, PEPD, PEPS, PSP, and PGP in mink: comparison with man and mouse

“…glutamale oxaloacclate transaminase-1 (GOTI) for chromosome 2. peptidase A (PEPA) for chromosome 4. superoxide dismutase-1 (SOD I) for chromosome 5, lactate dehydrogenase A (LDHA) lor chromosome 7. esterase D (ESDI for chromosome 8, lactate dehydrogenase B (LDHB) for chromosome 9, malate dehydrogenase-1 (NAD dependent) (M O R I) for chromosome 11, aeon base-1 (ACON1) for chromosome 12, peptidase C (PEPC) for chromosome 13 and glucose-6-phosphate dehydrogenase (G6PD) for the X chromosome. Electro phoresis and staining of the markers were performed according to the methods described previously (Rubtsov etal.. 1981a, b;1982: Gradov et al" 1983, Electrophoresis and staining were carried out for APRT (Nesterova ct al" 1987). for UM PH2 (Wilson ct al" 1986), for PF.PD and PEPS (Mullakandov cl al" 1986).…”

Regional assignments of eight genes on chromosome 2 in the American mink

“…has been detected in clone COI 13 by electrophoresis (Rubtsov et al, 1981b(Rubtsov et al, . 1982aGradov et al, 1983). Based on these data, we tentatively assigned PGD.…”

“…and ADK in the cell cultures were analyzed electrophoretically (Rubtsov et al, 1981b(Rubtsov et al, . 1982aGradov et al, 1983). Under the conditions utilized the differences between the electrophoretic mobilities of mink PGD.…”

“…hexokinase-1 (HK1), glutamate-oxaloacetate transaminase-1 (GOT1), purine nucleoside phosphorylase (NP). and inorganic pyrophosphatase (PP) on mink chromo some 2 (Rubtsov et al, 1981b(Rubtsov et al, , 1982aGradov et al, 1983).…”