Chromosome locations of genes controlling ‘purple leaf base’ in rye and wheat

Melz, G.; Thiele, V.

doi:10.1007/bf00027265

Cited by 17 publications

(8 citation statements)

References 12 publications

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“…The genetic mapping of An5 to the distal end of the long arm of chromosome 5R further specifies the localization of a gene of the anthocyanin biosynthesis pathway in rye, which has been assigned to chromosome 5R using trisomics of the anthocyaninless rye cv. “Esto” (Melz and Thiele, 1990). The recombination frequency between Ddw1 and Hp estimated in the present study compares well with previous results (Korzun et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…This material was advanced to nine F 4 : 5 families ( N = 697) derived from heterozygous Ddw1 genotypes of a segregating F 3 : 4 family (Supplementary Figure 1). In addition, two genotypes of the Peterhof rye stock collection, the inbred line l.7 (tall, smooth peduncle, green leaf base) and p. 83 (dwarf – carrying Ddw1 as well as (i) the GA-insensitive dwarfing gene compactum 2 ( ct2 ), (ii) hairy peduncle ( Hp ), and (iii) purple leaf base ( An5 , Melz and Thiele, 1990), were crossed. One single F1 plant from this cross, heterozygous for Ddw1 , ct2 , Hp , and An5 , was used to produce 101 F2 plants (population B) for genetic analyses and mapping purposes.…”

Section: Methodsmentioning

confidence: 99%

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Gene Expression Profiling and Fine Mapping Identifies a Gibberellin 2-Oxidase Gene Co-segregating With the Dominant Dwarfing Gene Ddw1 in Rye (Secale cereale L.)

et al. 2019

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The gibberellin (GA)-sensitive dwarfing gene Ddw1 provides an opportunity to genetically reduce plant height in rye. Genetic analysis in a population of recombinant inbred lines confirmed a monogenetic dominant inheritance of Ddw1 . Significant phenotypic differences in PH between homo- and heterozygotic genotypes indicate an incomplete dominance of Ddw1 . De novo transcriptome sequencing of Ddw1 mutant as well as tall genotypes resulted in 113,547 contigs with an average length of 318 bp covering 36.18 Mbp rye DNA. A hierarchical cluster analysis based on individual groups of rye homologs of functionally characterized rice genes controlling morphological or physiological traits including plant height, flowering time, and source activity identified the gene expression profile of stems at the begin of heading to most comprehensively mirror effects of Ddw1 . Genome-wide expression profiling identified 186 transcripts differentially expressed between semi-dwarf and tall genotypes in stems. In total, 29 novel markers have been established and mapped to a 27.2 cM segment in the distal part of the long arm of chromosome 5R. Ddw1 could be mapped within a 0.4 cM interval co-segregating with a marker representing the C20-GA2-oxidase gene ScGA2ox12 , that is up-regulated in stems of Ddw1 genotypes. The increased expression of ScGA2ox12 observed in semi-dwarf rye as well as structural alterations in transcript sequences associated with the ScGA2ox12 gene implicate, that Ddw1 is a dominant gain-of-function mutant. Integration of the target interval in the wheat reference genome sequence indicated perfect micro-colinearity between the Ddw1 locus and a 831 kb segment on chromosome 5A, which resides inside of a 11.21 Mb interval carrying the GA-sensitive dwarfing gene Rht12 in wheat. The potential of Ddw1 as a breeder’s option to improve lodging tolerance in rye is discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Gene Expression Profiling and Fine Mapping Identifies a Gibberellin 2-Oxidase Gene Co-segregating With the Dominant Dwarfing Gene Ddw1 in Rye (Secale cereale L.)

et al. 2019

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“…The genes Rc, Pc, Pan, Plb and Pls are closely linked to each other and represent homoeoloci on the short arms of chromosomes 7A, 7B, and 7D (Khlestkina et al 2002a(Khlestkina et al , b, 2008a(Khlestkina et al , 2009a. Genes Ra and Pp were not shown to cluster with these factors or with each other; nor have homoeoloci been reported for them (Gulyaeva 1984;Melz and Thiele 1990;Dobrovolskaya et al 2006).…”

Section: Introductionmentioning

confidence: 94%

Mapping genes controlling anthocyanin pigmentation on the glume and pericarp in tetraploid wheat (Triticum durum L.)

2009

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“…In rye, chromosome 4R controls anthocyanin pigmentation in the culm and coleoptiles (Zeller and Koller 1981;Miller 1984), whereas chromosomes 2R and 5R control that in seeds and auricles, respectively (De Vries and Sybenga 1984;Melz and Thiele 1990). The locus an controlling the trait anthocyaninless was mapped on chromosome 7R (De Vries and Sybenga 1984;Malyshev et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Anthocyanin biosynthesis genes location and expression in wheat–rye hybrids

2009

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Studies into gene expression in a foreign background contribute toward understanding of how genes derived from different species or genera manages to co-exist in a common nucleus, on the one hand, and help to estimate possible effectiveness of wide hybridization for cultivated plant improvement, on the other hand. The aim of this study was to investigate conservation of wheat and rye expression networks, using the anthocyanin biosynthesis pathway (ABP) genes as a model system. We isolated and analyzed ABP genes encoding enzymes acting at different steps of the pathway: chalcone-flavanone isomerase (CHI), flavanone 3-hydroxylase (F3H), anthocyanidin synthase (ANS), and anthocyanidin-3-glucoside rhamnosyltransferase (3RT). The rye ABP genes locations we determined (Chi on chromosome 5RL, F3h on 2RL, Ans on 6RL, 3Rt on 5RL, the regulatory Rc--red coleoptile--gene on 4RL) were in agreement with the rearrangements established between rye and wheat chromosomes. Expression of the ABP structural genes was studied in wheat-rye chromosome addition and substitution lines. F3h activation by the Rc gene was found to be critical for the red coleoptile trait formation. It was shown that the rye regulatory Rc gene can activate the wheat target gene F3h and vice versa wheat Rc induces expression of rye F3h. However, lower level of expression of rye F3h in comparison with that of the two wheat orthologues in the wheat-rye chromosome substitution line 2R(2D) was observed. Thus, although work of the wheat and rye ABP gene systems following the formation of wheat-rye hybrids is finely coordinated, some divergence exists between rye and wheat ABP genes, affecting level of gene expression.

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Chromosome locations of genes controlling ‘purple leaf base’ in rye and wheat

Cited by 17 publications

References 12 publications

Gene Expression Profiling and Fine Mapping Identifies a Gibberellin 2-Oxidase Gene Co-segregating With the Dominant Dwarfing Gene Ddw1 in Rye (Secale cereale L.)

Gene Expression Profiling and Fine Mapping Identifies a Gibberellin 2-Oxidase Gene Co-segregating With the Dominant Dwarfing Gene Ddw1 in Rye (Secale cereale L.)

Mapping genes controlling anthocyanin pigmentation on the glume and pericarp in tetraploid wheat (Triticum durum L.)

Anthocyanin biosynthesis genes location and expression in wheat–rye hybrids

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