Chromosome Manipulation Techniques and Applications to Aquaculture

Arai, Katsutoshi; Fujimoto, Takafumi

doi:10.1002/9781119127291.ch6

Cited by 21 publications

(19 citation statements)

References 126 publications

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“…Generally, fish with induced WGD through inhibition of the first cell division of the zygote, once the chromosomes have been duplicated shortly after fertilisation, are considered an important source of fertile diploid gametes and as such, they are of great scientific interest 57 . Extremely high embryonic and juvenile mortality rates is the greatest obstacle of such an approach 58 , not only in diploid teleosts but also in sturgeons, as reviewed by Havelka and Arai 59 . Such AWGD organisms may display altered cell architecture and genomic regulatory networks leading to dosage imbalances and abnormal expressions, as concluded by Yin et al 60 .…”

Section: Discussionmentioning

confidence: 99%

“…However, processes responsible for the appearance of mosaic individuals in this study are still not completely understood. Arai and Fujimoto 58 in their review pointed out the pivotal role of centriole behaviour among cellular mechanisms responsible for induced WGD, when a coexistence of monopolar and bipolar spindles in blastomeres may lead to diploid-tetraploid mosaicism. Analysis of ploidy level in six month old juveniles of Russian and Siberian sturgeon from groups subjected to AWGD showed no mosaicism, or at least no mosaic cells in the fin tissue and blood of studied individuals.…”

Section: Discussionmentioning

confidence: 99%

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Artificial whole genome duplication in paleopolyploid sturgeons yields highest documented chromosome number in vertebrates

Lebeda

Ráb

Majtánová

et al. 2020

Sci Rep

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Critically endangered sturgeons, having undergone three whole genome duplication events, represent an exceptional example of ploidy plasticity in vertebrates. Three extant ploidy groups, combined with autopolyploidization, interspecific hybridization and the fertility of hybrids are important issues in sturgeon conservation and aquaculture. Here we demonstrate that the sturgeon genome can undergo numerous alterations of ploidy without severe physiological consequences, producing progeny with a range of ploidy levels and extremely high chromosome numbers. Artificial suppression of the first mitotic division alone, or in combination with suppression of the second meiotic division of functionally tetraploid zygotes (4n, C-value = 4.15) of Siberian sturgeon Acipenser baerii and Russian sturgeon A. gueldenstaedtii resulted in progeny of various ploidy levels—diploid/hexaploid (2n/6n) mosaics, hexaploid, octoploid juveniles (8n), and dodecaploid (12n) larvae. Counts between 477 to 520 chromosomes in octoploid juveniles of both sturgeons confirmed the modal chromosome numbers of parental species had been doubled. This exceeds the highest previously documented chromosome count among vertebrates 2n ~ 446 in the cyprinid fish Ptychobarbus dipogon.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Artificial whole genome duplication in paleopolyploid sturgeons yields highest documented chromosome number in vertebrates

Lebeda

Ráb

Majtánová

et al. 2020

Sci Rep

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“…Finally, the basic cytogenetics information here provided, like the presence of sex chromosomes, could open a path for studies of genetic manipulation [ 77 , 78 ] or marker-assisted selection [ 79 ] in D. latifrons , aimed at improving productive characteristics, in order to make this species an alternative to tilapia in the Ecuadorian aquaculture industry. Indeed, the Pacific fat sleeper, due to its high tolerance to variations in salinity and temperature and its low oxygen requirement, may prove useful as aquaculture shifts in the face of climate change [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Sex Chromosomes and Internal Telomeric Sequences in Dormitator latifrons (Richardson 1844) (Eleotridae: Eleotrinae): An Insight into Their Origin in the Genus

Paim

Nirchio

Oliveira

et al. 2020

Genes

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The freshwater fish species Dormitator latifrons, commonly named the Pacific fat sleeper, is an important food resource in CentralSouth America, yet almost no genetic information on it is available. A cytogenetic analysis of this species was undertaken by standard and molecular techniques (chromosomal mapping of 18S rDNA, 5S rDNA, and telomeric repeats), aiming to describe the karyotype features, verify the presence of sex chromosomes described in congeneric species, and make inferences on chromosome evolution in the genus. The karyotype (2n = 46) is mainly composed of metacentric and submetacentic chromosomes, with nucleolar organizer regions (NORs) localized on the short arms of submetacentric pair 10. The presence of XX/XY sex chromosomes was observed, with the X chromosome carrying the 5S rDNA sequences. These heterochromosomes likely appeared before 1 million years ago, since they are shared with another derived Dormitator species (Dormitator maculatus) distributed in the Western Atlantic. Telomeric repeats hybridize to the terminal portions of almost all chromosomes; additional interstitial sites are present in the centromeric region, suggesting pericentromeric inversions as the main rearrangement mechanisms that has driven karyotypic evolution in the genus. The data provided here contribute to improving the cytogenetics knowledge of D. latifrons, offering basic information that could be useful in aquaculture farming of this neotropical fish.

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“…The induction of triploid hybrids (allo-triploids) has given rise to drastic recovery or increased survival potential in several inviable hybrids (Chevassus et al, 1983;Scheerer and Thorgaard, 1983;Arai, 1984Arai, , 1986Arai, , 1988Ueda et al, 1984;Parsons et al, 1986;Yamano et al, 1988;Seeb et al, 1988;Gray et al, 1993). These types of viable allo-triploid hybrid strains exhibit sterility and have been commercially farmed to support the vitalization of local economies in Japan (Arai, 2000(Arai, , 2001; Arai and Fujimoto, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…As seen in the inviable hybrids from certain combinations of fish species, artificially induced haploid embryos exhibit severe abnormalities collectively referred to as haploid syndrome. These abnormalities include dwarfism, microcephaly, microphthalmia and oedema, therefore almost all gynogenetic or androgenetic haploids die before hatching or first feeding (Arai, 2000(Arai, , 2001Arai and Fujimoto, 2019). In goldfish, gynogenetically induced haploid embryos die before or around hatching, however haploid cells were able to survive when they were transplanted into diploid blastula embryos and, consequently, viable haploiddiploid chimeras can be produced (Tanaka et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Developmental potential of somatic and germ cells of hybrids betweenCarassius auratusfemales andHemigrammocypris rasborellamales

Naya

Matsunaga

Shimizu

et al. 2020

Zygote

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Summary The cause of hybrid sterility and inviability has not been analyzed in the fin-fish hybrid, although large numbers of hybridizations have been carried out. In this study, we produced allo-diploid hybrids by cross-fertilization between female goldfish (Carassius auratus) and male golden venus chub (Hemigrammocypris rasborella). Inviability of these hybrids was due to breakage of the enveloping layer during epiboly or due to malformation with serious cardiac oedema around the hatching stage. Spontaneous allo-triploid hybrids with two sets of the goldfish genome and one set of the golden venus chub genome developed normally and survived beyond the feeding stage. This improved survival was confirmed by generating heat-shock-induced allo-triploid hybrids that possessed an extra goldfish genome. When inviable allo-diploid hybrid cells were transplanted into goldfish host embryos at the blastula stage, these embryos hatched normally, incorporating the allo-diploid cells. These allo-diploid hybrid cells persisted, and were genetically detected in a 6-month-old fish. In contrast, primordial germ cells taken from allo-diploid hybrids and transplanted into goldfish hosts at the blastula stage had disappeared by 10 days post-fertilization, even under chimeric conditions. In allo-triploid hybrid embryos, germ cells proliferated in the gonad, but had disappeared by 10 weeks post-fertilization. These results showed that while hybrid germ cells are inviable even in chimeric conditions, hybrid somatic cells remain viable.

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Chromosome Manipulation Techniques and Applications to Aquaculture

Cited by 21 publications

References 126 publications

Artificial whole genome duplication in paleopolyploid sturgeons yields highest documented chromosome number in vertebrates

Artificial whole genome duplication in paleopolyploid sturgeons yields highest documented chromosome number in vertebrates

Sex Chromosomes and Internal Telomeric Sequences in Dormitator latifrons (Richardson 1844) (Eleotridae: Eleotrinae): An Insight into Their Origin in the Genus

Developmental potential of somatic and germ cells of hybrids betweenCarassius auratusfemales andHemigrammocypris rasborellamales

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