Chromosome number of<i>Micranthes nelsoniana</i>(D. Don) Small (<i>Saxifragaceae</i>) in northeast Asia

Fukuda, Tomoko; Andreeva, Elena D.; Taran, Aleksandr; Takahashi, Hideki; Ikeda, Hiroshi

doi:10.1080/00087114.2016.1215091

Cited by 2 publications

(1 citation statement)

References 5 publications

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“…Plant materials were collected in the field and cultivated in Botanic Garden, Hokkaido University. The methods for the observation of somatic chromosome numbers followed Fukuda et al (2007Fukuda et al ( , 2016 with some modifications. Root tips were pretreated in 0.002 M 8-hydroxyquinoline solution for 10 hours at 4°C, and fixed in Farmer's solution (glacial acetic acid: 99% ethanol = 1 : 3) for more than 16 hours at 4°C.…”

Section: Chromosome Observationmentioning

confidence: 99%

Molecular and cytological evidences denied the immediate-hybrid hypothesis for Saxifraga yuparensis (sect. Bronchiales, Saxifragaceae) endemic to Mt. Yubari in Hokkaido, northern Japan

et al. 2018

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SAYA TAMURA, TOMOKO FUKUDA, ELENA A. PIMENOVA, EKATERINA A. PETRUNENKO, PAVEL V. KRESTOV, SVETLANA N. BONDARCHUK, OLGA A. CHERNYAGINA, YOSHIHISA SUYAMA, YOSHIHIRO TSUNAMOTO, AYUMU MATSUO, HAYATO TSUBOI, HIDEKI TAKAHASHI, KEN SATO, YOKO NISHIKAWA, TAKASHI SHIMAMURA, HIROKO FUJITA & KOH NAKAMURA An alpine plant Saxifraga yuparensis is endemic to a scree consisting of greenschist of Mt. Yubari in Hokkaido, Japan and it has been proposed as an immediate hybrid derived from two species of the same section Bronchiales based on morphological intermediacy: namely S. nishidae, a diploid species endemic to a nearby cliff composed of greenschist and tetraploid S. rebunshirensis comparatively broadly distributed in Japan and Russian Far East. Saxifraga yuparensis is red-listed and it is crucial for conservation planning to clarify whether this is an immediate hybrid and lacks a unique gene pool. The immediate-hybrid hypothesis was tested by molecular and cytological data. In nuclear ribosomal and chloroplast DNA trees based on maximum parsimony and Bayesian criteria, S. yuparensis and S. rebunshirensis formed a clade with several other congeners while S. nishidae formed another distinct clade. Genome-wide SNP data clearly separated these three species in principal coordinate space, placing S. yuparensis not in-between of S. rebunshirensis and S. nishidae. Chromosome observation indicated that S. yuparensis is tetraploid, not triploid directly derived from diploid-tetraploid crossing. Additionally, observation of herbarium specimens revealed that leaf apex shape of S. yuparensis fell within the variation of S. rebunshirensis. These results indicate that S. yuparensis is not an immediate hybrid of S. rebunshirensis and S. nishidae but a distinct lineage and an extremely narrow endemic species, that deserves for intensive conservation.

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Section: Chromosome Observationmentioning

confidence: 99%

Molecular and cytological evidences denied the immediate-hybrid hypothesis for Saxifraga yuparensis (sect. Bronchiales, Saxifragaceae) endemic to Mt. Yubari in Hokkaido, northern Japan

et al. 2018

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Phylogeography and chromosome number variation in Micranthes nelsoniana and related species (Saxifragaceae) in Northeast Asia

Fukuda,

Ishikawa,

Chernyagina

et al. 2023

Botanical Journal of the Linnean Society

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Micranthes nelsoniana possesses multiple different variants and numerous chromosomes. Based on the internal transcribed spacer (ITS) and chloroplast (cp)DNA sequences, the phylogeography of M. nelsoniana and its relatives in Northeast Asia was investigated, with extensive sampling around the Kuril Islands. The Arctic–Asian continent and a clade of marginal islands were the two main groupings that comprised the ITS phylogenetic tree. The island clade was separated into five well-supported clades: Kamchatka and Hokkaido highlands, Kuril–Aleutian Islands, southern Kuril Islands, Japanese archipelago, and Primorye region. Micranthes fusca was found in Japan and in the southern Kuril Islands. It is a separate species that created several types of hybrids between M. nelsoniana in the centre of the Kuril Islands based on a comparison of the ITS and cpDNA networks. Micranthes nelsoniana and M. ohwii appear to have hybridized in the northern Kuril Islands. Cytological investigation on the local species of M. nelsoniana showed that the chromosomal numbers are: 2n = 24, 26, 28, 30, 50, and 80. Among them, two usual numbers to this area, 2n = 24 and 50, appear to encourage interspecific gene exchange. The genomes of Hokkaido plants with high chromosome counts were cloned, revealing that they contained genes of both continental and marginal origins. This study revealed the crucial role of marginal islands along Northeast Asia in the genetic diversity of M. nelsoniana and related species.

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Chromosome number ofMicranthes nelsoniana(D. Don) Small (Saxifragaceae) in northeast Asia

Cited by 2 publications

References 5 publications

Molecular and cytological evidences denied the immediate-hybrid hypothesis for Saxifraga yuparensis (sect. Bronchiales, Saxifragaceae) endemic to Mt. Yubari in Hokkaido, northern Japan

Molecular and cytological evidences denied the immediate-hybrid hypothesis for Saxifraga yuparensis (sect. Bronchiales, Saxifragaceae) endemic to Mt. Yubari in Hokkaido, northern Japan

Phylogeography and chromosome number variation in Micranthes nelsoniana and related species (Saxifragaceae) in Northeast Asia

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